Fibrin adhesive from snake venom: the effect of adding epsilon-aminocaproic acid, tranexamic acid, and aprotinin for coaptation of wound edges in rat skin incisions

Thomazini-Santos, I. A.

doi:10.1590/s0104-79302001000100011

Cited by 7 publications

(4 citation statements)

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“…In 1998, Thomazini-Santos et al [26] showed that the cryoprecipitate extracted from bubaline blood had higher concentrations of fibrinogen and better performance when compared with blood from humans, bovines, ovine or equines. Additionally, they concluded that antifibrinolytic agents were not required to this sealant to achieve successful coaptation of skin surgical edges [27]. Stolf et al [28] applied for the first time this new sealant in humans as an alternative to conventional suture in the nasolabial region, and described a good adhesive capacity and healing of this material.…”

Section: Introductionmentioning

confidence: 99%

Unique heterologous fibrin biopolymer with hemostatic, adhesive, sealant, scaffold and drug delivery properties: a systematic review

Buchaim

Cassaro

Shindo

et al. 2019

J. Venom. Anim. Toxins incl. Trop. Dis

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Fibrin biopolymers, previously referred as "fibrin glue" or "fibrin sealants", are natural biomaterials with diverse applications on health. They have hemostatic, adhesive, sealant, scaffold and drug delivery properties and have become widely used in medical and dental procedures. Historically, these biomaterials are produced from human fibrinogen and human or animal thrombin, and the possibility of transmission of infectious diseases by human blood is not ruled out. In the 1990s, to overcome this problem, a new heterologous biomaterial composed of a thrombin-like enzyme purified from Crotalus durissus terrificus venom and a cryoprecipitate rich in fibrinogen extracted from buffaloes Bubalus bubalis blood has been proposed. Therefore, a systematic review of studies on exclusively heterologous fibrin sealants published between 1989 and 2018 was carried out using the following databases: PubMed, SciELO and Google Scholar. The keyword used was "heterologous fibrin sealant". The search resulted in 35 scientific papers in PubMed, four in SciELO and 674 in Google Scholar. After applying the inclusion/exclusion criteria and complete reading of the articles, 30 studies were selected, which formed the basis of this systematic review. It has been observed that the only completely heterologous sealant is the one produced by CEVAP/UNESP. This heterologous biopolymer is proven effective by several studies published in refereed scientific journals. In addition, clinical trials phase I/II for the treatment of chronic venous ulcers authorized by the Brazilian Health Regulatory Agency (ANVISA) were completed. Preliminary results have indicated a safe and promising effective product. Phase III clinical trials will be proposed and required to validate these preliminary findings.

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Section: Introductionmentioning

confidence: 99%

Unique heterologous fibrin biopolymer with hemostatic, adhesive, sealant, scaffold and drug delivery properties: a systematic review

Buchaim

Cassaro

Shindo

et al. 2019

J. Venom. Anim. Toxins incl. Trop. Dis

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“…Gasparotto et al [29] showed an excellent interaction of this FS with the ASC, due to its ability to induce the spontaneous adipogenic, chondrogenic and osteogenic lineages differentiation. This new FS is composed of a fibrinogen-rich cryoprecipitate extracted from the Bubalus bubalis buffalo’s blood in association with a serine protease (a thrombin-like enzyme) extracted from Crotalus durissus terrificus venom [30,31,32,33]). According to Ferreira et al [34], a thrombin-like enzyme, in the presence of calcium, acts upon the fibrinogen molecule transforming it into fibrin monomers forming a stable clot with adhesive, hemostatic and sealant effects [32,33,35].…”

Section: Introductionmentioning

confidence: 99%

Transected Tendon Treated with a New Fibrin Sealant Alone or Associated with Adipose-Derived Stem Cells

Frauz

Teodoro

Carneiro

et al. 2019

Cells

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Tissue engineering and cell-based therapy combine techniques that create biocompatible materials for cell survival, which can improve tendon repair. This study seeks to use a new fibrin sealant (FS) derived from the venom of Crotalus durissus terrificus, a biodegradable three-dimensional scaffolding produced from animal components only, associated with adipose-derived stem cells (ASC) for application in tendons injuries, considered a common and serious orthopedic problem. Lewis rats had tendons distributed in five groups: normal (N), transected (T), transected and FS (FS) or ASC (ASC) or with FS and ASC (FS + ASC). The in vivo imaging showed higher quantification of transplanted PKH26-labeled ASC in tendons of FS + ASC compared to ASC on the 14th day after transection. A small number of Iba1 labeled macrophages carrying PKH26 signal, probably due to phagocytosis of dead ASC, were observed in tendons of transected groups. ASC up-regulated the Tenomodulin gene expression in the transection region when compared to N, T and FS groups and the expression of TIMP-2 and Scleraxis genes in relation to the N group. FS group presented a greater organization of collagen fibers, followed by FS + ASC and ASC in comparison to N. Tendons from ASC group presented higher hydroxyproline concentration in relation to N and the transected tendons of T, FS and FS + ASC had a higher amount of collagen I and tenomodulin in comparison to N group. Although no marked differences were observed in the other biomechanical parameters, T group had higher value of maximum load compared to the groups ASC and FS + ASC. In conclusion, the FS kept constant the number of transplanted ASC in the transected region until the 14th day after injury. Our data suggest this FS to be a good scaffold for treatment during tendon repair because it was the most effective one regarding tendon organization recovering, followed by the FS treatment associated with ASC and finally by the transplanted ASC on the 21st day. Further investigations in long-term time points of the tendon repair are needed to analyze if the higher tissue organization found with the FS scaffold will improve the biomechanics of the tendons.

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“…This reaction is enhanced by the activation of factor XIII, which also participates in the synthesis of collagen, stimulating fibroblast proliferation and contributing to tissue healing. Factor XIII is also an excellent hemostatic agent 11 . However, the use of this commercial fibrin sealant is limited by the 12 .…”

Section: Introductionmentioning

confidence: 99%

Hydroxyapatite and a New Fibrin Sealant Derived from Snake Venom as Scaffold to Treatment of Cranial Defects in Rats

et al. 2015

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Biomaterials are used as a promising alternative to bone grafts, including bioceramics whose composition resembles that of bone and fibrin sealants due to their hemostatic properties. The objective was to evaluate the repair of cranial defects in 40 rats, grafted with hydroxyapatite and a new fibrin sealant derived from snake venom. The animals were divided into four groups: C (control, no graft); Ha (hydroxyapatite); FS (fibrin sealant), and HaFS (hydroxyapatite and fibrin sealant). The animals were euthanized 2 and 6 weeks after surgery and wound area were submitted to analysis. After 2 weeks, immature bone was formed from the borders of the defect and in groups Ha and HaFS, few hydroxyapatite particles were surrounded by new bone. After 6 weeks, the new bone was mature and surrounded several hydroxyapatite particles, without connective tissue interposition and the volume of new bone was higher in HaFS group. The hydroxyapatite in combination with the new fibrin sealant accelerates bone repair.

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Fibrin adhesive from snake venom: the effect of adding epsilon-aminocaproic acid, tranexamic acid, and aprotinin for coaptation of wound edges in rat skin incisions

Cited by 7 publications

References 0 publications

Unique heterologous fibrin biopolymer with hemostatic, adhesive, sealant, scaffold and drug delivery properties: a systematic review

Unique heterologous fibrin biopolymer with hemostatic, adhesive, sealant, scaffold and drug delivery properties: a systematic review

Transected Tendon Treated with a New Fibrin Sealant Alone or Associated with Adipose-Derived Stem Cells

Hydroxyapatite and a New Fibrin Sealant Derived from Snake Venom as Scaffold to Treatment of Cranial Defects in Rats

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