Novel Snake Venom Proteins Cytolytic to Cancer Cells in Vitro and in Vivo Systems

Lipps, B. V.

doi:10.1590/s0104-79301999000200005

Cited by 19 publications

(17 citation statements)

References 7 publications

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“…on adenocarcinoma cells. Thereafter many reports have established the anticancer potential of venoms of different species of Elapidae, Viperidae, Crotalidae snakes [102][103][104][105][106][107] and Hydrophis spimlis sea snake [108,109] and assigned it to phospholipase activity. Scorpions venom has been used by traditional and folk medicine in India, China, Africa and Cuba.…”

Section: Chemotherapeutical Agents 41 Drugs Of Natural Originmentioning

confidence: 99%

Anticancer Drug Discovery — From Serendipity to Rational Design

Latosińska¹,

Latosińska²

2013

Drug Discovery

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the overall survival time increased significantly, especially in high-developed countries, but in fact the metastases, not the cancer itself, are the major cause of death. In 1971, only 50% of people diagnosed with the cancer went on to live at least five years, while nowadays, the fiveyear survival rate is 63% [2]. However if a cancer has spread the chances of survival are only scarcely better than in the 1970s. These numbers indicate that although the knowledge about cancer in the last two decades raised, even to a larger degree than in all preceding centuries, but the problem of cancer diseases persists and our knowledge is still insufficient to solve it. Despite the remarkable progress in cancer prevention, early detection, and treatment, made during the last few decades, the methods of cancer diagnosis and treatment are still not sufficiently specific and effective thus cancer still takes a heavy toll.Not so long ago in the beginning of 20 th century, neither carcinogens nor cellular targets were identified while the treatment was carried out exclusively by surgeries or natural products selected by trial and error. Modern cancer therapy based on the so-called holistic approachthe combined use of surgical methods, radiotherapy, chemotherapy, hormonal therapy and immunotherapy -is applied in the treatment of cancer at most stages. In fact this approach originated from the ancient Sumerian, Akkadian, Babylonian, Assyrian and Egyptian medicine, and was largely influenced by the Roman and Greek ideas concerning anatomy, physiology as well as the achievements of practical medicine and natural science. The chemotherapy, hormonal therapy, immunotherapy and radiotherapy as the methods of cancer treatment joined to the oldest surgical one only in the 20 th c. An important component of the combined therapy, but sometimes when cancer had already metastasised, the only available therapeutic method, is chemotherapy using natural or synthetic anticancer drugs and treated as curative, palliative, adjuvant or neoadjuvant. Over the centuries anticancer drugs evolved from natural products, discovered mainly from green plants and minerals to fully chemically synthesized chemotherapeutic agents. However, even today drugs of natural origin play an important role in the treatment of cancer as 14 of them were on the list of the top 35 drugs worldwide sales [4]. The process of anticancer drug discovery leading from natural products to chemotherapeutic agents, often illicitly limited only to cytostatic and antiproliferative, has evolved from serendipity to rational design based on advances in chemistry, physics and biology in a long and complicated process. Nowadays both cancer itself and anticancer drugs are investigated at the molecular level thus methods of drug discovery have changed diametrically. The dominant direction of contemporary aniticancer drug discovery is the search for the possibilities to influence the pathogenetic mechanisms specific of the tumour structures at the cellular and molecular levels, which require the kn...

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Section: Chemotherapeutical Agents 41 Drugs Of Natural Originmentioning

confidence: 99%

Anticancer Drug Discovery — From Serendipity to Rational Design

Latosińska¹,

Latosińska²

2013

Drug Discovery

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“…Studies have shown that venoms from snake species posses lytic activity against tumoral cells (Lipps 1999;Silva et al 2002) and antibacterial action (Stilles et al 1991) in vitro.…”

Section: Introductionmentioning

confidence: 99%

In vitro effects of Crotalus durissus terrificus and Bothrops jararaca venoms on Giardia duodenalis trophozoites

et al. 2005

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Considering the snake venoms' pharmacological properties and chemotherapeutic potential as well as the need for new alternatives for Giardia infection treatment, the present study was carried out aiming to evaluate the in vitro effects of crude Crotalus durissus terrificus and Bothrops jararaca venoms on the growth and adherence of Giardia duodenalis trophozoites. Trophozoites (10(6)) were exposed to serial twofold dilutions of C. durissus terrificus and B. jararaca venoms that ranged from 3.125 to 200 microg/ml and from 5 to 320 microg/ml, respectively. The two venoms inhibited the growth of trophozoites, and the level of inhibition varied according to the assayed concentrations and incubation times. The highest reduction of parasite growth was observed with increasing concentrations of the venoms. No effect on parasite adherence was observed. Light microscope observations revealed changes of the pear-shape aspect of the cell and reduction of flagellar beating frequency in the great part of the trophozoites. This is the first attempt to evaluate the in vitro effects of snake venoms on Giardia trophozoites. The findings stress the need for more investigations and prompt us to perform further studies to identify active principles with antigiardial effect.

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“…This was confirmed by MTT assay, the release of LDH is considered to be of necrotic pathway and the treated cells showing very less levels of LDH after 24 h suggests that the event of cell death is by apoptosis. Lipps observed similar results for the two protein fractions from snake venom, Atroporin and Kaotree on SP/2 cell line [24]. Moreover, Greel and Vaux [25] have suggested that a cancer cell treated with a potentially lethal toxin will not die because of the direct effects of toxin, but will activate its suicide mechanism to die earlier by apoptosis.…”

Section: Discussionmentioning

confidence: 81%

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Balasubashini¹,

Karthigayan²,

Somasundaram³

et al. 2006

J Carcinog

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The present study is an attempt to evaluate the antiproliferative potential of peptide (7.6 kDa) from lionfish (Pterios volitans) venom on cultured HEp2 and HeLa cells. Different dose of purified peptide (1, 2 and 4 μg/ml) at different time points (12, 24 and 36 hrs) were tested for antiproliferative index of the peptide. Among them, 2 μg/ml at 24 hrs was found to effectively inhibit cancer cell growth in vitro and did not cause any adverse effect on normal human lymphocytes. Apoptosis was examined by propidium iodide staining, confirmed by the expression of caspase-8 and caspase-3, down regulation of Bcl-2 expression and DNA fragmentation in treated cells, when compared to untreated HEp2 and HeLa cells. Thus fish venom peptide was found to selectively induce apoptosis in cancer cell.

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Novel Snake Venom Proteins Cytolytic to Cancer Cells in Vitro and in Vivo Systems

Cited by 19 publications

References 7 publications

Anticancer Drug Discovery — From Serendipity to Rational Design

Anticancer Drug Discovery — From Serendipity to Rational Design

In vitro effects of Crotalus durissus terrificus and Bothrops jararaca venoms on Giardia duodenalis trophozoites

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