Production of cellulose-degrading enzyme on sisal and other agro-industrial residues using a new Brazilian actinobacteria strain Streptomyces sp. SLBA-08

Macedo, E. P.; Cerqueira, C. L. O.; Souza, Diogo Angeli Jacinto de; Bispo, A. S. R.; Coelho, R. R. R.; Nascimento, Rodrigo Pires do

doi:10.1590/s0104-66322013000400005

Cited by 25 publications

(12 citation statements)

References 28 publications

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“…Ethanol is mostly produced from sugar and starch feedstocks; however, the interest in using biomass and bioenergy has been initiated due to the recognition that the global crude oil reserve is finite, and its depletion is occurring much faster than previously predicted (Bai et al, 2008;Macedo et al, 2013;Scholl et al, 2015a). Lignocellulosic materials have a high potential for ethanol production, do not compete with food production, could lead to the use of large quantities of agroindustrial wastes and could use marginal or degraded agricultural lands for growing energy crops (Eliana et al, 2014;Idrees et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Use of elephant grass (Pennisetum purpureum) as substrate for cellulase and xylanase production in solid-state cultivation by Penicillium echinulatum

Menegol

Scholl

Dillon

et al. 2017

Braz. J. Chem. Eng.

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-The high cost of the enzymes used for the hydrolysis of lignocellulosic biomass is one of the bottlenecks in the production of second-generation ethanol and the use of local biomass, such elephant grass, can help to reduce this cost. In this investigation elephant grass biomass was evaluated as a carbon and inducer source of production of cellulases and xylanases by the fungus Penicillium echinulatum strain 9A02S1 in solid-state cultivation. The highest filter paper activity (13.26 U.g -1 dry matter) and β-glucosidase activity (138.34 U.g -1 dry matter) were obtained on the fifth day of cultivation, in medium containing biomass pre-treated with sulfuric acid and untreated, respectively. The highest endoglucanase activity was 158.44 U.g -1 dry matter in the presence of elephant grass pre-treated with sulfuric acid. The xylanase activity was highest with medium that was formulated with 75% untreated elephant grass and 25% wheat bran (372.62 U.g -1 dry matter). In conclusion, the results showed that it is possible to obtain large amounts of cellulases and xylanases using a cosmopolitan and very low cost substrate such as elephant grass.

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Section: Introductionmentioning

confidence: 99%

Use of elephant grass (Pennisetum purpureum) as substrate for cellulase and xylanase production in solid-state cultivation by Penicillium echinulatum

Menegol

Scholl

Dillon

et al. 2017

Braz. J. Chem. Eng.

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“…Most fungi are capable of degrading both amorphous and crystalline cellulose, while the majority of the bacterial strains effectively hydrolyze only amorphous cellulose (CMC, carboxymethylcellulose). Some bacterial genera such as Bacillus and Paenibacillus are capable of degrading both CMC and Avicel (Afzal et al, 2010), as well as lignocellulosic waste from the agro-industry (Macedo et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

PRODUCTION AND CHARACTERIZATION OF THERMOPHILIC CARBOXYMETHYL CELLULASE SYNTHESIZED BY Bacillus sp. GROWING ON SUGARCANE BAGASSE IN SUBMERGED FERMENTATION

Padilha

Carvalho

Dias

et al. 2015

Braz. J. Chem. Eng.

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-The production and characterization of cellulase from thermophilic strain Bacillus sp. C1AC5507 was studied. For enzyme production, sugarcane bagasse was used as carbon source. The produced carboxymethyl cellulase (CMCase) had a molecular weight around 55 kDa and its activity varied between 0.14 and 0.37 IU mL -1 in conditions predicted by Response Surface Methodology. The optimum temperature and pH for the CMCase production were 70 °C and 7.0, respectively. The enzyme activity was inhibited mostly by Cu +2 and activated mostly by Co +2 , Mn 2+ , Ca +2 and Fe +3 . Our findings provide a contribution to the use of natural wastes such as sugarcane bagasse as substrate for growth and production of thermophilic CMCase. Further optimization to increase the production of cellulase enables the use in industrial applications.

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“…In another study, the half-life of endoglucanase was obtained from Streptomyces sp. SLBA-08 at 8 h and 50 °C (Macedo et al 2013). Fig.…”

Section: Characterization Of Endoxylanase and Endoglucanasementioning

confidence: 94%

Production and Characterization of Partially Purified Thermostable Endoxylanase and Endoglucanase from Novel Actinomadura geliboluensis and the Biotechnological Applications in the Saccharification of Lignocellulosic Biomass

Adıgüzel¹,

Tunçer²

2017

BioResources

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Extracellular endoxylanase and endoglucanase from halo-and thermotolerant Actinomadura geliboluensis were produced, purified, characterized, and used in the saccharification of native and pretreated lignocellulosic biomasses. The molecular mass of endoxylanase and endoglucanase were 30 and 38 kDa, respectively. The optimum pH and temperature values for both endoxylanase and endoglucanase activities were pH 6.0 and 60 °C, respectively. They were both stable within a pH range of 4.0 to 8.0 and up to 70 °C. The half-lives of endoxylanase and endoglucanase at 70 °C were calculated as 180 min and 60 min, while their half-lives at 80 °C were detected as 60 min and 50 min, respectively. Both the endoxylanase and endoglucanase obeyed Michaelis-Menten kinetics. . The potential application of endoxylanase and endoglucanase in saccharification of lignocellulosic biomass was further evaluated. The reduced sugar was 265.12 mg/g biomass after both endoxylanase and endoglucanase were incubated with wheat straw, which was pretreated by 1 % NaOH at 121 °C for 15 min. Endoxylanase and endoglucanase were produced from novel A. geliboluensis, which could potentially be used in biotechnological applications.

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Production of cellulose-degrading enzyme on sisal and other agro-industrial residues using a new Brazilian actinobacteria strain Streptomyces sp. SLBA-08

Cited by 25 publications

References 28 publications

Use of elephant grass (Pennisetum purpureum) as substrate for cellulase and xylanase production in solid-state cultivation by Penicillium echinulatum

Use of elephant grass (Pennisetum purpureum) as substrate for cellulase and xylanase production in solid-state cultivation by Penicillium echinulatum

PRODUCTION AND CHARACTERIZATION OF THERMOPHILIC CARBOXYMETHYL CELLULASE SYNTHESIZED BY Bacillus sp. GROWING ON SUGARCANE BAGASSE IN SUBMERGED FERMENTATION

Production and Characterization of Partially Purified Thermostable Endoxylanase and Endoglucanase from Novel Actinomadura geliboluensis and the Biotechnological Applications in the Saccharification of Lignocellulosic Biomass

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