Separation of l-tryptophan present in an aromatic amino acids mixture in a four-column simulated moving bed: experimental and simulation studies

Abstract

-An experimental and theoretical study is presented for the fractionation of L-Tryptophan (Trp) from L-phenylalanine (Phe) and L-tyrosine (Tyr). A Simulated-Moving Bed (SMB) with four-column system was tested, and the results are presented as effluent histories of the raffinate and extract ports. L-phenylalanine and L-tyrosine were recovered as major products in the raffinate, while L-Tryptophan was recovered at the extract. To simulate the L-Tryptophan separation, a general rate model was used to represent the mass transfer phenomena that occur in each individual column of the SMB, for each solute present in the multicomponent system. A hybrid method was used to solve the model. An analytic solution was used for the intra-particle concentration, and was correlated to the liquid bed concentration by Duhamel's theorem. The results from simulation are compared with the experimental data presented in this work.

Taxol® Separation in a Simulated Moving Bed

“…The Taxol ® separation simulation is based on laboratory-scale, four-column SMB founded in the Cremasco et al (2009aCremasco et al ( , 2009b as shown in Figure 4. Each column presents the same Fig.…”

“…4. SMB apparatus: valves system (Cremasco et al 2009a(Cremasco et al , 2009b characteristics which are presented in Table 1. The system utilizes six eight-port rotary valves, four manual shut-off valves, and four pumps.…”