-An experimental and theoretical study is presented for the fractionation of L-Tryptophan (Trp) from L-phenylalanine (Phe) and L-tyrosine (Tyr). A Simulated-Moving Bed (SMB) with four-column system was tested, and the results are presented as effluent histories of the raffinate and extract ports. L-phenylalanine and L-tyrosine were recovered as major products in the raffinate, while L-Tryptophan was recovered at the extract. To simulate the L-Tryptophan separation, a general rate model was used to represent the mass transfer phenomena that occur in each individual column of the SMB, for each solute present in the multicomponent system. A hybrid method was used to solve the model. An analytic solution was used for the intra-particle concentration, and was correlated to the liquid bed concentration by Duhamel's theorem. The results from simulation are compared with the experimental data presented in this work.
-In this communication, the method proposed by Cremasco et al. (2003) is applied to predict single and low concentration pulse chromatography. In previous work, a general rate model was presented to describe the breakthrough curve, where a hybrid solution was proposed for the linear adsorption. The liquid phase concentration inside the particle was found analytically and related with the bed liquid phase through Duhamel´s Theorem, while the bulk-phase equation was solved by a numerical method. In this paper, this method is applied to describe pulse chromatography of solutes that present linear adsorption isotherms. The simulated results of pulse chromatography are compared with experimental ones for aromatic amino acid experiments from literature.
-In this paper, a new resolution method for the dynamic simulation of a simulated moving bed is presented. A general rate model for each solute and each column is presented for the case of a linear isotherm. A hybrid resolution is developed, in which an analytical solution is used for the solid stationary phase, while the mobile fluid phase concentrations are obtained by numerical resolution. It is illustrated, through a numerical study, that the resolution method is robust. The method is validated by comparison with previously published experimental results. It is also shown that these numerical simulations are obtained much faster than those based on the physical separation process, allowing the use of this resolution method for on-line applications and process control.
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