A kinetic model for growth and biosynthesis of medium-chain-length poly-(3-hydroxyalkanoates) in Pseudomonas putida

Annuar, Mohamad Suffian Mohamad; Tan, İlhan; Ibrahim, Shaliza; Ramachandran, K.B.

doi:10.1590/s0104-66322008000200001

Cited by 33 publications

(28 citation statements)

References 17 publications

(42 reference statements)

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“…A good R 2 (0.96) for ethanol production and a significant ANOVA test confirmed that the model provides the relevant prediction The same results were obtained in several researches using the same model (Annuar et al, 2008). In addition, Jiménez-Islas et al (2014) found the effects of pH and temperature on ethanol production from red beet juice by t wo strains: S. cerevisiae ITD00196 and S. cerevisiae A TCC 9763.…”

Section: Ethanol Productionsupporting

confidence: 67%

“…The coefficient of deter mination R 2 w as estimated to assess the accuracy of the estimated parameters achieved by fitting the experimental values to the proposed mathematical models. If R 2 approximate to 1, this coefficient justifies an excellent consistency of these equations (Annuar et al, 2008). Furthermore, the ANOVA …”

Section: Model Of Param Eters Estim Ationmentioning

confidence: 71%

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Kinetic models and parameters estimation study of biomass and ethanol production from inulin by Pichia caribbica (KC977491)

Ali¹,

Hiligsmann²,

Outili³

et al. 2017

Afr. J. Biotechnol.

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The growth kinetics and modeling of ethanol production from inulin by Pichia caribbica (KC977491) were studied in a batch system. Unstructured models were proposed using the logistic equation for growth, the Luedeking-Piret equation for ethanol production and modified Leudeking-Piret model for substrate consumption. Kinetic parameters (X 0 , μ m , m, n, p and q) were determined by nonlinear regression, using Levenberg-Marquart method implemented in a Mathcad program. Since the production of ethanol was associated with P. caribbica cell growth, a good agreement between model predictions and experimental data was obtained. Indeed, significant R 2 values of 0.91, 0.96, and 0.95 were observed for biomass, ethanol production and substrate consumption, respectively. Furthermore, analysi s of variance (ANOVA) w as also used to validate the proposed models. According to the obtained results, the predicted kinetic values and experimental data agreed well . Finally, it is possible to predict the development of P. caribbica using these models.

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Section: Ethanol Productionsupporting

confidence: 67%

Section: Model Of Param Eters Estim Ationmentioning

confidence: 71%

Kinetic models and parameters estimation study of biomass and ethanol production from inulin by Pichia caribbica (KC977491)

Ali¹,

Hiligsmann²,

Outili³

et al. 2017

Afr. J. Biotechnol.

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“…Several kinetics models to describe the cell growth during biosynthesis of PHA in different bacterial species have been proposed (Annuar et al , 2008; Agarry et al , 2009; Baei et al , 2011). The culture conditions, nutrient limitation and the type of feeding substrates were reported to influence the structure, quantity and quality of polymer produced.…”

Section: Introductionmentioning

confidence: 99%

“…For instance, Li et al (2011), while studying the synthesis of poly-3-hydroxybutyrate- co -3-hydroxyvelarate by mutant Pseudomonas putida KTOY06ΔC reported that change in substrates or precursors in fermentation media resulted in a defined composition of copolymer. Annuar et al (2008) reported that ammonium limitation in a medium efficiently encouraged PHA production in P. putida PGA 1. Recently, Rai et al (2011) reported the induction of synthesis of polyhydroxy-octanoate homopolymer in Pseudomonas mendocina CH50 by feeding sodium octanoate using two-stage seed culture techniques.…”

Section: Introductionmentioning

confidence: 99%

Growth kinetics, effect of carbon substrate in biosynthesis of mcl-PHA by Pseudomonas putida Bet001

Gumel¹,

Annuar²,

Heidelberg³

2014

Braz. J. Microbiol.

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Growth associated biosynthesis of medium chain length poly-3-hydroxyalkanoates (mcl-PHA) in Pseudomonas putida Bet001 isolated from palm oil mill effluent was studied. Models with substrate inhibition terms described well the kinetics of its growth. Selected fatty acids (C8:0 to C18:1) and ammonium were used as carbon and nitrogen sources during growth and PHA biosynthesis, resulting in PHA accumulation of about 50 to 69% (w/w) and PHA yields ranging from 10.12 g L−1 to 15.45 g L−1, respectively. The monomer composition of the PHA ranges from C4 to C14, and was strongly influenced by the type of carbon substrate fed. Interestingly, an odd carbon chain length (C7) monomer was also detected when C18:1 was fed. Polymer showed melting temperature (Tm) of 42.0 (± 0.2) °C, glass transition temperature (Tg) of −1.0 (± 0.2) °C and endothermic melting enthalpy of fusion (ΔHf) of 110.3 (± 0.1) J g−1. The molecular weight (Mw) range of the polymer was relatively narrow between 55 to 77 kDa.

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“…Cited from [11] which supported the above conclusion by stating that one of the phases for producing starter is by growing the microbes in the proper medium and incubation process until it reached the optimum growth rate.…”

Section: Resultsmentioning

confidence: 80%

Kinetic Growth of Saccharomyces cerevisiae in Non Dairy Creamer Wastewater Medium

2016

J Environ Stud

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Efforts in non dairy creamer (NDC) wastewater treatment can be performed by converting it into raw materials, i.e. as a medium for microorganisms growth. This research had an objective of examining the growth kinetics of Saccharomyces cerevisiae in the fermentation of the non dairy creamer wastewater for protein production. Kinetic study of the growth and fermentation is necessary as an introduction to understanding each process of the fermentation. The research applied an Experimental Design with a full factorial design. Treatments during the experiment consisted of P1 (NDC medium concentration of 25%), P2 (NDC medium concentration of 50%), P3 (NDC medium concentration of 75%), and P4 (NDC medium concentration of 100%). The cellular concentrate intakes were 105, 106, 107, and 108 cell/ml. The experiment resulted in specific growth rate (µ) of 0.240868 (hour-1), maximum specific growth rate (µmax) of 55.7479 (hour-1), with the highest protein contents of 22.15 mg/l at the concentration medium of 75%. IntroductionNon dairy creamer wastewater that migrates from waste disposal has been proven to cause pollution due to inadequate treatment. In addition to reducing water body quality, the wastewater also has bad smell, which also impacts the affected environment. The smell comes from organic compounds within the wastewater. Therefore, proper treatment is important to convert the wastewater into valuable raw materials.There have been many studies concerning wastewater as a more affordable growth medium for microorganisms in order to produce single cell proteins. However, none of the studies has done so with non dairy creamer wastewater. The use of Saccharomyces cerevisiae in the treatment of non dairy creamer wastewater is expected to have a promising prospect in producing single cell proteins. Single cell proteins are dry cells, which can be found in khamir, bacteria, and algae. These biomass microorganisms are potential sources of proteins for human food and livestock feed. The single cell proteins can be an alternative to fulfill the future needs for proteins because they contain particular proteins as well as carbohydrates, fat, mineral, and other nutrients for both human and animals, cited from [1][2][3]. The use of Saccharomyces cerevisiae in the non dairy creamer wastewater treatment is expected to be effective in the production of the single cell proteins.According to laboratory analysis, the non dairy creamer wastewater contained organic matters, in particular carbon in the form of carbohydrate, protein, and fat, so that they have a great potential to be a growth medium for microorganisms. One of the treatments of the non dairy creamer industry wastewater can be converting the wastewater into raw materials. These raw materials are useful for the medium of the microorganism fermentation developed in the production of the single cell proteins.The fermentation process can be assessed through fermentation kinetics. Kinetics study of the growth and fermentation is important as an introduction to understand...

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A kinetic model for growth and biosynthesis of medium-chain-length poly-(3-hydroxyalkanoates) in Pseudomonas putida

Cited by 33 publications

References 17 publications

Kinetic models and parameters estimation study of biomass and ethanol production from inulin by Pichia caribbica (KC977491)

Kinetic models and parameters estimation study of biomass and ethanol production from inulin by Pichia caribbica (KC977491)

Growth kinetics, effect of carbon substrate in biosynthesis of mcl-PHA by Pseudomonas putida Bet001

Kinetic Growth of Saccharomyces cerevisiae in Non Dairy Creamer Wastewater Medium

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