Semicontinuous cultivation of the cyanobacterium Spirulina platensis in a closed photobioreactor

Reichert, Carolina C.; Reinehr, Christian Oliveira; Costa, Jorge Alberto Vieira

doi:10.1590/s0104-66322006000100003

Cited by 79 publications

(53 citation statements)

References 13 publications

(12 reference statements)

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“…Since the cyanobacteria strains were reared at similar radiation conditions and in full nutrient medium, the negative growth rate results cannot be linked to non-adaptation towards the light regime, temperature or medium. In contrast, Moreno et al [63] observed production rates for D. lemmermannii (Tables A.1 and A.3), which is comparable to the studies of Reichert et al [79] with Spirulina cultures. Overall, in all treatments and on both Day 7 and Day 14, N. spumigena had statistically significantly higher biovolume and specific growth rate than both A. flos-aquae and D. lemmermannii, but not TFA per biovolume (Table A.1).…”

Section: Biovolume and Growthsupporting

confidence: 66%

Cyanobacteria in Scandinavian coastal waters — A potential source for biofuels and fatty acids?

et al. 2014

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Since land-based biofuel production competes with conventional food production, a water-based biomass and biofuel production from cyanobacteria offers large potential. This study investigates the application potential of cyanobacteria for fuel production and by-products by mimicking nutrient depleted environmental conditions. Three Baltic cyanobacteria strains (Aphanizomenon flos-aquae, Dolichospermum lemmermannii and Nodularia spumigena) were inoculated in full nutrient levels, as well as phosphorus and nitrogen depleted medium, before being monitored for 14 days. For screening reasons, multiple parameters such as fatty acids, photosynthetic pigments including phycobilins, biovolume, photosynthetic activity, inorganic nutrients, particulate organic carbon, nitrogen and phosphorous were investigated every seven days. We observed a strong negative relationship between lipid content, growth and nutrient availability, resulting in high lipid and pigment production in combination with a limited growth rate in nutrient depleted treatments. Our results suggest that cultivation and harvest of bloom-forming cyanobacteria for fuel and by-product production are feasible in Scandinavia, but strongly depends on the desired compounds and biomass. Each cyanobacteria species originally has a species-specific chemical fingerprint that may be modified by rearing conditions and harvesting period to meet the needs of the consumer. This leads to important conclusions regarding future culturing conditions and biomass production of the desired compounds.

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Section: Biovolume and Growthsupporting

confidence: 66%

Cyanobacteria in Scandinavian coastal waters — A potential source for biofuels and fatty acids?

et al. 2014

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“…(Table II). The highest μ max value was 0.22 d Ð1 for strain LEB 18, which was higher than the 0.11 d Ð1 obtained for A. platensis in semi-continuous cultivation by Reichert et al (2006). Thus we found that the highest μ max value was obtained Table II.…”

Section: Resultsmentioning

confidence: 50%

Isolation and Characterization of a New Arthrospira Strain

Morais

Reichert

Dalcanton

et al. 2008

Zeitschrift Für Naturforschung C

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A filamentous microorganism, morphologically similar to the cyanobacterium Arthrospira, was isolated from Mangueira Lagoon in Brazil, from which Arthrospira has not previously been isolated. Random amplified polymorphic DNA (RAPD) comparison with the standard Arthrospira platensis strains LEB 52 and Paracas indicated that the organism isolated was an Arthrospira isolate, which we denominated strain LEB 18. The RAPD analysis showed conserved sequences which indicated that the three strains belonged to the same genus, and were all Arthrospira species, but there were sufficient differences between them suggesting that they were separate strains. The strain LEB 18 was cultivated in undiluted Zarrouk medium and in 60% and 20% (v/v) Zarrouk medium diluted with sterilized Mangueira Lagoon water (MLW) using illuminance rates of 32.5, 45.5 and 58.5 μmol m Ð2 s Ð1 according to a complete 3 2 factorial design with a triplicate central point. The strains LEB 52 and Paracas were cultived in the conditions central point. Our new isolate produced the highest specific growth rate (μ max = 0.22 d Ð1) in 60% Zarrouk medium diluted with MLW and illuminated with 58.5 μmol m Ð2 s Ð1 and the highest protein content (86.0% w/w).

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“…Even when urea is used as a nitrogen source, ammonia is formed either by its hydrolysis under alkaline conditions (Danesi et al, 2002) or by urease activity (Shimamatsu, 2004). Spirulina platensis can be cultivated by batch, continuous, semicontinuous (Reichert et al, 2006) and fed-batch processes . The latter process, when conducted at both a suitable feeding time and feeding rate to supply the nitrogen source according to A. platensis demand, can avoid any inhibitory ammonia concentration in the medium (Bezerra et al, 2008;Carvalho et al, 2004;Matsudo et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

GROWTH AND COMPOSITION OF Arthrospira (Spirulina) platensis IN A TUBULAR PHOTOBIOREACTOR USING AMMONIUM NITRATE AS THE NITROGEN SOURCE IN A FED-BATCH PROCESS

Cruz-Martínez

Jesus

Matsudo

et al. 2015

Braz. J. Chem. Eng.

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-NH 4 NO 3 simultaneously provides a readily assimilable nitrogen source (ammonia) and a reserve of nitrogen (nitrate), allowing for an increase in Arthrospira platensis biomass production while reducing the cost of the cultivation medium. In this study, a 2 2 plus star central composite experimental design combined with response surface methodology was employed to analyze the influence of light intensity (I) and the total amount of added NH 4 NO 3 (M t ) on a bench-scale tubular photobioreactor for fed-batch cultures. The maximum cell concentration (X m ), cell productivity (P X ) and biomass yield on nitrogen (Y X/N ) were evaluated, as were the protein and lipid contents. Under optimized conditions (I = 148 µmol·photons·m -2 ·s -1 and M t = 9.7 mM NH 4 NO 3 ), X m = 4710 ±34.4 mg·L -1 , P X = 478.9 ±3.8 mg·L -1 ·d -1 and Y X/N = 15.87 ±0.13 mg·mg -1 were obtained. The best conditions for protein content in the biomass (63.2%) were not the same as those that maximized cell growth (I = 180 µmol·photons·m -2 ·s -1 and M t = 22.5 mM NH 4 NO 3 ). Based on these results, it is possible to conclude that ammonium nitrate is an interesting alternate nitrogen source for the cultivation of A. platensis in a fed-batch process and could be used for other photosynthetic microorganisms.

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Semicontinuous cultivation of the cyanobacterium Spirulina platensis in a closed photobioreactor

Cited by 79 publications

References 13 publications

Cyanobacteria in Scandinavian coastal waters — A potential source for biofuels and fatty acids?

Cyanobacteria in Scandinavian coastal waters — A potential source for biofuels and fatty acids?

Isolation and Characterization of a New Arthrospira Strain

GROWTH AND COMPOSITION OF Arthrospira (Spirulina) platensis IN A TUBULAR PHOTOBIOREACTOR USING AMMONIUM NITRATE AS THE NITROGEN SOURCE IN A FED-BATCH PROCESS

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