Isolation and Characterization of a New Arthrospira Strain

Abstract

A filamentous microorganism, morphologically similar to the cyanobacterium Arthrospira, was isolated from Mangueira Lagoon in Brazil, from which Arthrospira has not previously been isolated. Random amplified polymorphic DNA (RAPD) comparison with the standard Arthrospira platensis strains LEB 52 and Paracas indicated that the organism isolated was an Arthrospira isolate, which we denominated strain LEB 18. The RAPD analysis showed conserved sequences which indicated that the three strains belonged to the same genus, and were all Arthrospira species, but there were sufficient differences between them suggesting that they were separate strains. The strain LEB 18 was cultivated in undiluted Zarrouk medium and in 60% and 20% (v/v) Zarrouk medium diluted with sterilized Mangueira Lagoon water (MLW) using illuminance rates of 32.5, 45.5 and 58.5 μmol m Ð2 s Ð1 according to a complete 3 2 factorial design with a triplicate central point. The strains LEB 52 and Paracas were cultived in the conditions central point. Our new isolate produced the highest specific growth rate (μ max = 0.22 d Ð1) in 60% Zarrouk medium diluted with MLW and illuminated with 58.5 μmol m Ð2 s Ð1 and the highest protein content (86.0% w/w).

Effect of Spirulina addition on the physicochemical and structural properties of extruded snacks

“…LEB 18, isolated from Mangueira Lagoon (Morais et al, 2008) and produced at the Pilot Plant of the Laboratory of Biochemical Engineering, located in Santa Vitória do Palmar, Rio Grande do Sul, Brazil (33°30'13"S and 53°08'59"W). The flours used were commercial organic corn flour (Indústria e Comércio de Erva Mate Salet, Coronel…”

“…As an alternative to the supply of nutrients, Spirulina has a high concentration of digestible protein (Morais et al, 2008), besides being a source of essential fatty acids, vitamins, minerals and pigments.…”

New technologies from the bioworld: selection of biopolymer-producing microalgae

“…[23] , Spirulina sp. LEB 18 [24] and Synechococcus nidulans [7] belong to the Collection of Strains of the Laboratory of Biochemical Engineering of the Federal University of Rio Grande (FURG). Spirulina sp.…”

Use of Solid Waste from Thermoelectric Plants for the Cultivation of Microalgae

“…LEB 18, isolated from Mangueira lagoon, latitude 33º31'08"S and longitude 53º22'05"W. Chlorella fusca LEB 111 was isolated from the pond for stabilization of effluents and waste at the President Medici Thermoelectric Power Plant (UTPM), latitude 24°36'13''S and longitude 52º32'43''W (Candiota RS); (Morais et al 2008 (Rippka 1979).…”