Inulinase from Kluyveromyces marxianus: culture medium composition and enzyme extraction

Abstract

K. marxianus DSM 70106 was cultivated for inulinase production in a medium containing 2.0 g/L of yeast extract, 5.0 g/L of peptone and salts. The addition of corn steep liquor did not increase enzyme production. Inulin, as the main carbon source, afforded higher inulinase production than glucose, fructose, sucrose, lactose, maltose and starch. Glucose, fructose and sucrose reduced enzyme production by 46, 58 and 71%, respectively. By using the best culture medium enzyme activity remained stable for 22 months at 4oC; while at -18oC it decreased by 10%. Maximal activity was found in the pH range of 3.5 to 5.0 and at temperatures from 50 to 60oC. Flocculation was used for cell separation. Shifting the pH was more efficient than using polyelectrolytes, CaCl2, bentonite and Fe2O3. Recovery of inulinase by AOT(sodium di-2-ethylhexyl sulfosuccinate)-reversed micelles yielded up to ~20%

Production of inulinase by solid-state fermentation: effect of process parameters on production and preliminary characterization of enzyme preparations

“…The optimum activity pH and temperature change among the works are dependent on the microorganism, substrate, control of process variables, etc. Pessoa and Vitolo [22] characterized an inulinase produced by K. marxianus ATCC 52466 and found the optimal pH and temperature of 5.0 and 50°C, respectively. Cazetta et al [23] characterized an inulinase produced by K. marxianus var.…”

Comparison of the Gene Expression Patterns of Alcohol Dehydrogenase Isozymes in the Thermotolerant YeastKluyveromyces marxianusand Their Physiological Functions

“…18,19) This yeast is also capable of growing on lactose as sole carbon source, which provides the possibility of utilizing cheese whey as a cheap source of nutrients for its cultivation. 20) K. marxianus has been investigated for the production of bioingredients, 18) cell proteins, 21,22) inulinase, 16,[23][24][25] and -galactosidase, [26][27][28] and even for use as a baker's yeast. 29) In spite of these potential applications of K. marxianus, there is a lack of information in the literature concerning its physiology.…”

Produção de concentrados de frutose por inulinases de Penicillium janczewskii e atividade sobre o nível de glicose plasmática em ratos diabéticos

“…O meio de cultura utilizado pelos autores era enriquecido com peptona como fonte de nitrogênio. PESSOA JR. & VITOLO [24] também consideraram Kluyveromyces marxianus um microrganismo promissor para produção de inulinases em escala industrial. Esses autores enriqueceram o meio de cultura com diferentes fontes de nitrogênio (peptona ou extrato de levedura) e, conseguiram aumentar a produção de inulinases, obtendo uma enzima com atividade entre 12 -26U/mL de meio de cultura.…”

“…Sua utilização na indústria de alimentos e bebidas vem crescendo de forma acentuada, pois esse açúcar não apresenta problemas de cristalização como a sacarose [12]. Além disso, a frutose é tolerada por pacientes diabéticos e está relacionada com o aumento da absorção de ferro em crianças [24]. Dessa forma, a frutose vem sendo recomendada como adoçante alternativo para diabéticos, uma vez que promove diminuição do nível de glicose plasmática [1] e, diferentemente da glicose, pode ser metabolizada independentemente da insulina [2].…”