QUANTIFICATION OF THE EFFECT OF SOME OPERATIONAL VARIABLES ON THE CELL GROWTH YIELD (Yx/s) OF Penilcilium chrysogenum BY SURFACE RESPONSE ANALYSIS

Abstract

The yield coefficient Yx/s was correlated with variables such as temperature (22.8-29.2 oC), concentrations of corn steep liquor (6.4-57.4 g/L), ammonium sulfate (5.6-18.4 g/L), sucrose (4.0-36.0 g/L) and soybean oil (3.0-7.0 g/L), all important variables in the growth stage of the Penicillium chrysogenum fungus. An empirical model was obtained by response surface statistical experimental design. The tests were performed with a complex culture medium in a rotatory shaker. The highest Yx/s values were obtained at low sucrose concentrations (~4.0 g/L) and high corn steep liquor concentrations (~57.4 g/L). Temperature, ammonium sulfate and soybean oil concentrations showed no significant effect on Yx/

Media optimization for β-Fructofuranosidase production by Aspergillus oryzae

“…Optimization studies are carried out using response surface methodology (RSM) which is a mathematical and statistical technique widely used to determine the effects of several variables and their interactions and to optimize different biotechnological processes (Oprime and Suazo, 1997;Burkert et al, 2006;Zafar et al, 2010;Coelho et al, 2011;). RSM has been extensively applied to optimize culture medium and other process parameters for the production of β-fructofuranosidase (Chen 1998), lipase (He and Tan 2006;Liu et al, 2006), tannase (Battestin and Macedo 2007), α-amylase (Rao and Satyanarayana 2007), α-cyclodextrin glucanotransferase (Ibrahim et al, 2005), dextran dextrinase (Naessens et al, 2004) and chitinase (Nawani and Kapadnis 2005).…”