<FONT FACE=Symbol>b</font>-GALACTOSIDASE IMMOBILIZATION ON CONTROLLED PORE SILICA

Abstract

The immobilization of <FONT FACE="Symbol">b</font> -galactosidase from Kluyveromyces fragilis on controlled pore silica was investigated. Immobilization was performed on amino silica activated with glutaraldehyde and the product was applied to the hydrolysis of lactose of whey. The behaviors of the soluble and immobilized enzyme were compared by using whey and a lactose solution as the substrate. With the aim of optimizing the method, parameters such as the amount of glutaraldehyde and the size of the particles were evaluated by comparing activities and stabilities on batch and continuously fluidized bed reactor

Experimental study of lactose hydrolysis and separation in cstr-uf membrane reactor

“…Furthermore, a high amount of lactose in dairy products like ice cream, condensed milk, etc., leads to an undesirable grainy texture. The objective of many researches is reduction of the lactose amount in waste streams or dairy products through methods like hydrolysis of lactose or separation processes (Cuartas-Uribe et al, 2009;Marwaha and Kennedy, 1988;Tragardh, 1991;Trevisan et al, 1997). It should be mentioned that hydrolysis reduces lactose losses and converts it to beneficial products at the same time.…”