Use of a lactic acid plus lactoserum intimate liquid soap for external hygiene in the prevention of bacterial vaginosis recurrence after metronidazole oral treatment

Bahamondes, M. Valeria; Portugal, Priscila Mendes; Brolazo, Eliane Melo; Simões, J. A.; Bahamondes, Luís

doi:10.1590/s0104-42302011000400015

Cited by 19 publications

(16 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…65 Other unblinded studies have reported that lactate gel inserted into the vagina for 7 days is as effective as oral metronidazole in the treatment of BV 66 and that topical use of lactic acid and lactoserum intimate liquid soap following standard oral metronidazole treatment reduced BV recurrence. 67 Further blinded controlled studies are warranted to examine the potential for l -lactic acid, particularly applied continuously via an intravaginal ring, for BV treatment and prevention.…”

Section: Discussionmentioning

confidence: 99%

Vaginal concentrations of lactic acid potently inactivate HIV

Aldunate

Tyssen

Johnson

et al. 2013

Journal of Antimicrobial Chemotherapy

139

158

View full text Add to dashboard Cite

ObjectivesWhen Lactobacillus spp. dominate the vaginal microbiota of women of reproductive age they acidify the vagina to pH <4.0 by producing ∼1% lactic acid in a nearly racemic mixture of d- and l-isomers. We determined the HIV virucidal activity of racemic lactic acid, and its d- and l-isomers, compared with acetic acid and acidity alone (by the addition of HCl).MethodsHIV-1 and HIV-2 were transiently treated with acids in the absence or presence of human genital secretions at 37°C for different time intervals, then immediately neutralized and residual infectivity determined in the TZM-bl reporter cell line.Resultsl-lactic acid at 0.3% (w/w) was 17-fold more potent than d-lactic acid in inactivating HIVBa-L. Complete inactivation of different HIV-1 subtypes and HIV-2 was achieved with ≥0.4% (w/w) l-lactic acid. At a typical vaginal pH of 3.8, l-lactic acid at 1% (w/w) more potently and rapidly inactivated HIVBa-L and HIV-1 transmitter/founder strains compared with 1% (w/w) acetic acid and with acidity alone, all adjusted to pH 3.8. A final concentration of 1% (w/w) l-lactic acid maximally inactivated HIVBa-L in the presence of cervicovaginal secretions and seminal plasma. The anti-HIV activity of l-lactic acid was pH dependent, being abrogated at neutral pH, indicating that its virucidal activity is mediated by protonated lactic acid and not the lactate anion.Conclusionsl-lactic acid at physiological concentrations demonstrates potent HIV virucidal activity distinct from acidity alone and greater than acetic acid, suggesting a protective role in the sexual transmission of HIV.

show abstract

Section: Discussionmentioning

confidence: 99%

Vaginal concentrations of lactic acid potently inactivate HIV

Aldunate

Tyssen

Johnson

et al. 2013

Journal of Antimicrobial Chemotherapy

139

158

View full text Add to dashboard Cite

show abstract

“…However, the results concerning this strategy are controversial since acidification strategies alone, using acetic acid (Holley et al, 2004 ) or acid-buffering formulation (Simoes et al, 2006 ) showed to be somewhat ineffective against BV. Recently, Bahamondes and colleagues verified that a soap containing lactic acid and lactoserum could be used for external intimate hygiene, reducing BV recurrence after treatment with oral metronidazole (Bahamondes et al, 2011 ). Interestingly, vitamin C, when coated with silicon, allowed the constant release of the active agent, resulting in a long-lasting vaginal low pH and prevention of vaginal irritation (Polatti et al, 2006 ).…”

Section: Emerging Therapeutic Alternatives Against Bvmentioning

confidence: 99%

Bacterial Vaginosis Biofilms: Challenges to Current Therapies and Emerging Solutions

et al. 2016

View full text Add to dashboard Cite

Bacterial vaginosis (BV) is the most common genital tract infection in women during their reproductive years and it has been associated with serious health complications, such as preterm delivery and acquisition or transmission of several sexually transmitted agents. BV is characterized by a reduction of beneficial lactobacilli and a significant increase in number of anaerobic bacteria, including Gardnerella vaginalis, Atopobium vaginae, Mobiluncus spp., Bacteroides spp. and Prevotella spp.. Being polymicrobial in nature, BV etiology remains unclear. However, it is certain that BV involves the presence of a thick vaginal multi-species biofilm, where G. vaginalis is the predominant species. Similar to what happens in many other biofilm-related infections, standard antibiotics, like metronidazole, are unable to fully eradicate the vaginal biofilm, which can explain the high recurrence rates of BV. Furthermore, antibiotic therapy can also cause a negative impact on the healthy vaginal microflora. These issues sparked the interest in developing alternative therapeutic strategies. This review provides a quick synopsis of the currently approved and available antibiotics for BV treatment while presenting an overview of novel strategies that are being explored for the treatment of this disorder, with special focus on natural compounds that are able to overcome biofilm-associated antibiotic resistance.

show abstract

“…Vaginal lactic acid is important, as it correlates with vaginal health by inhibiting the growth of bacteria known to cause vulvovaginal infections [20] and may also play a part in local immune defense [24]. External feminine washes, particularly those containing lactic acid and formulated to an acidic pH that enhances skin homeostasis, are considered more appropriate than internal washes or douches and may be a useful adjunct therapy for women with vaginal infections or taking antibiotics [21].…”

Section: Discussionmentioning

confidence: 99%

“…A novel gel wash formulation containing lactic acid (pH 4.2) intended for daily external use was formulated to provide gentle cleansing, freshness, and antimicrobial protection to help maintain a healthy balance of the intimate skin area for women. The gel wash contains 2% lactic acid, a key natural antimicrobial ingredient that correlates with vulvovaginal health, as lactic acid helps maintain an acidic pH in the vulvovaginal area to augment skin homeostasis and prevent the growth of harmful bacteria [20][21][22]. The formulation was tested in vitro following standardized ASTM (American Society for Testing and Materials) test methodology (ASTM E2315-03 Standard Guide for Assessment of Antimicrobial Activity Using a Time-Kill Procedure) and demonstrated effective antimicrobial activity (minimum of 1 log kill) against fungi (Candida albicans), Gram-positive (including activity against Group B Streptococcus and Staphylococcus aureus) and Gram-negative bacteria (including Escherichia coli; data not shown).…”

Section: Introductionmentioning

confidence: 99%

A 28 Day Clinical Assessment of a Lactic Acid-containing Antimicrobial Intimate Gel Wash Formulation on Skin Tolerance and Impact on the Vulvar Microbiome

et al. 2020

View full text Add to dashboard Cite

While intimate feminine hygiene products are widely used as part of daily cleansing routines, little is known about how these products impact the vulvovaginal area and its microbiome stability. This 4 week clinical study assessed tolerance of a novel gel wash containing lactic acid (pH 4.2) for external daily use when used on the external genital area and its effects on skin moisturization, vulvar skin pH, and the vulvar microbiome. After a 7 day pre-study conditioning period, 36 healthy females in three balanced age groups (18–29, 30–44, and 45–55 years) used the gel wash to cleanse their external genital area (mons pubis and vulva) and entire body at least once per day for 28 days. Skin tolerance of the gel wash was assessed by the gynecologist. Effects of the gel wash on vulvar skin microbiota were studied by performing bacterial 16S rRNA and fungal internal transcribed spacer (ITS) microbial richness and diversity analysis. Based on gynecologic assessment after 28 days of use, the gel wash showed acceptable tolerance, with no signs of increased dryness, redness, edema, itching, stinging, or burning. Use of the gel wash was associated with a significant increase in both short-term (single application) and longer-term (daily use for 28 days) skin moisturization. There was no significant change in vulvar skin pH over time with daily product use, and the gel wash did not significantly affect the natural vulvar microbiome species richness or diversity for bacteria or fungi. Results showed that this gel wash is a mild, moisturizing cleanser that maintains the natural pH and microbial diversity of vulvar skin. To our knowledge, this was the first study to assess the effect of an antimicrobial feminine gel wash on the natural pH and vulvar microbiome habitat of the skin using bacterial 16S rRNA and fungal ITS genetic sequencing techniques.

show abstract

Use of a lactic acid plus lactoserum intimate liquid soap for external hygiene in the prevention of bacterial vaginosis recurrence after metronidazole oral treatment

Abstract: A lactic acid plus lactoserum liquid soap for external intimate hygiene may be an option for the prevention of BV recurrence after treatment and cure with oral metronidazole.

Cited by 19 publications

References 20 publications

Vaginal concentrations of lactic acid potently inactivate HIV

Vaginal concentrations of lactic acid potently inactivate HIV

Bacterial Vaginosis Biofilms: Challenges to Current Therapies and Emerging Solutions

A 28 Day Clinical Assessment of a Lactic Acid-containing Antimicrobial Intimate Gel Wash Formulation on Skin Tolerance and Impact on the Vulvar Microbiome

Contact Info

Product

Resources

About