Efeito da colestase nos aspectos funcionais e morfológicos renais após nefrectomia

Lima, Daniel Xavier; Petroianu, Andy; Rodrigues, Kelly Cristine de Lacerda; Salles, Paulo Guilherme de Oliveira

doi:10.1590/s0104-42302008000600015

Cited by 1 publication

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References 11 publications

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“…Many articles available in several bases of research (Gonçalves et al, 2007;Lima et al, 2008;Duarte et al 2009;Messias et al, 2010;Melo et al, 2012;Taiwo Idowu et al, 2013;Achuba;Ogwumu, 2014;Lima et al, 2014;Mulata et al, 2015) show that it is still common the use counters to determine the blood cell count in animals, however, many of them correspond to standard equipment for human blood and not for laboratory animals. In this context, rodents are the most used animals for scientific studies in several areas as they have similar genetic and physiological characteristics to humans (Harkness;Wagner, 1993;Cubas et al, 2007, Melo et al, 2012Lima et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Automated method and not automated: the evaluation of hematological parameters of rats

Messias¹,

Messias²,

Farias³

et al. 2017

Revista Brasileira De Ciência Veterinária

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ResumoO objetivo deste trabalho foi verificar em sangue de rato a compatibilidade entre a metodologia automatizada e a não automatizada. Foram realizadas contagens das variáveis hematológicas de 10 ratos Wistar, Rattus norvegicus. Para o método automatizado foram utilizados os aparelhos ABX-Pentra-60c+ e Celldyn-3200. Para o método não automatizado foi utilizado o hemocitômetro nas contagens de eritrócitos, de leucócitos e de plaquetas, utilizando o líquido de Hayem (1:200), o líquido de Türk (1:20) e o líquido de Brecher e Cronkite (1:200), respectivamente. A determinação do hematócrito foi obtida utilizando-se uma centrífuga de micro-hematócrito, a concentração de hemoglobina pelo método de cianometa-hemoglobina (líquido de Drabkin) e os índices hematimétricos absolutos calculados através dos resultados da série vermelha. A contagem diferencial de leucócitos foi realizada em extensões de sangue (panótico rápido). Os dados foram analisados através da variância -ANOVA, seguida pelo teste Tukey, com nível de significância de 5%. Os valores obtidos pelo hemocitômetro comparadas aos contadores hematológicos mostraram diferenças significativas (p < 0,05) para neutrófilos, eosinófilos e basófilos. Os resultados permitiram concluir que as contagens realizadas com os aparelhos utilizados foram confiáveis e rápidas, mas não são indicadas para a contagem diferencial de leucócito.Palavras-chave: automação, hematologia, hemograma, sangue. AbstractThe aim of this study was to verify blood compatibility between automated and non-automated methodology in rats. Counts of haematological variables of 10 Wistar rat, Rattus norvegicus were made. For the automated method were used the ABXPentra-60c + equipment and Celldyn-3200. For non-automated method the hemocytometer was used to count the erythrocytes, leukocytes and platelets using the Hayem liquid (1: 200), the Türk liquid (1:20) and the Brecher and Cronkite liquid (1: 200), respectively. The determination of the hematocrit was obtained by using a microhematocrit centrifuge, hemoglobin concentration by cyanmethaemoglobin method (Drabkin liquid) and absolute RBC indices calculated by the results of red series. The differential leukocyte count was performed on blood extensions (fast Panotic). The data was analyzed through variance -ANOVA and then by Tukey test at 5% significance level. The values obtained by hemocytometer compared to hematological counters revealed significant differences (p < 0.05) for neutrophils, eosinophils and basophils. Through the results obtained, it was concluded that the counts made with the devices used were reliable and fast, but are not indicated for the differential count of white blood cell.

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Section: Introductionmentioning

confidence: 99%

Automated method and not automated: the evaluation of hematological parameters of rats

Messias¹,

Messias²,

Farias³

et al. 2017

Revista Brasileira De Ciência Veterinária

View full text Add to dashboard Cite

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Efeito da colestase nos aspectos funcionais e morfológicos renais após nefrectomia

Abstract: Cholestasis does not affect the weight, function or the compensatory renal growth of the remnant kidney after unilateral nephrectomy.

Cited by 1 publication

References 11 publications

Automated method and not automated: the evaluation of hematological parameters of rats

Automated method and not automated: the evaluation of hematological parameters of rats

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