Análise histológica e morfométrica da área cruenta tratada com membrana de silicone: estudo em ratos

Mendes, Eduardo Carlos Da Silveira; Viterbo, Fausto; Rosa, Celina Santaella

doi:10.1590/s0104-42302007000500014

Cited by 4 publications

(2 citation statements)

References 18 publications

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“…The removal of 50% from the incision injury tissue and 50% from the intact skin was considered for the procedure, with a safety margin of 1 cm around the wound core and the sample fixed in 10% formalin 7 . The biopsied rats were euthanized with thiopental intraperitoneally 90 mg / kg when the sample collection ended, which totaled 9 animals (3 animals of each group GC, GL and GA) …”

Section: Macroscopic Evaluationmentioning

confidence: 99%

Evaluation of wound healing treated with latex derived from rubber trees and Aloe Vera extract in rats

et al. 2016

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PURPOSE:To compare the use of latex derivative and Aloe vera extract to wound healing. METHODS:Twenty one rats were randomly divided into three groups and each one had a wound made by incision. The treatment consisted in: derivative of latex (GL), Aloe vera extract (GA) and saline solution (GC). The wound area was measured on the 7th, 14th and 21st days and macroscopic and microscopic evaluation were done. RESULTS:The comparison between the measurements of the wounds presented statistical difference in GC and GA from the 7th day of evaluation and GL from the 14th day. The extent of the wound was significantly smaller by the 7th day in GL. Histologically, in GL, the neovascularization was significant on the 7th, 14th and 21st days. On the 21st day the scar was large and little mature. In GA and GC, the findings were similar on the 7th, 14th and 21st days with a slight better organization of skin and collagen on the 21st in GA.CONCLUSIONS: Statistical analysis did not allow for the definition of the best topical agent. The latex had the highest angiogenesis, but a possible foreign body granuloma. Aloe vera has revealed a healing process adequated temporally in histology.

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Section: Macroscopic Evaluationmentioning

confidence: 99%

Evaluation of wound healing treated with latex derived from rubber trees and Aloe Vera extract in rats

et al. 2016

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“…Materials such as hyaluronic acid, fibrin, fibrinogen and collagen have been tested as carriers for drug delivery systems [1,2,3]. Collagen 2 is a potentially useful biomaterial since it is a major constituent of the connective tissue.…”

Section: Introductionmentioning

confidence: 99%

Validation of a UV-VIS Spectrophotometric method for the determination of usnic acid /collagen-based membranes

Nunes

Jesus²,

Bezerra³

et al. 2015

Scientia Plena

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O ácido úsnico (AU) é um metabólito secundário de líquens que tem demonstrado potenciais efeitos farmacológicos, tais como atividade antimicrobiana, antiviral, antiproliferativa e anti-inflamatória. O colágeno, por sua vez, é um dos biomateriais mais utilizados visando o desenvolvimento de sistema de liberação de fármacos. Sendo assim, o objetivo deste estudo foi desenvolver e validar um método analítico por espectrofotometria na região do ultravioleta (UV) para determinação de concentrações de AU em membranas de colágeno. Os parâmetros de validação foram determinados de acordo com a Agência Nacional de Vigilância Sanitária (ANVISA). A especificidade revelou que os excipientes na formulação não interferiram na análise. A linearidade nas concentrações de 2-10 µg/mL apresentou um coeficiente de correlação de 0,9994. O método mostrou excelente repetibilidade (desvio-padrão relativo < 1,0%). A precisão revelou uma recuperação média percentual de 100,43%. O método foi robusto para a variação de temperatura e solvente. Os limites de detecção e de quantificação foram 0,109 e 0,364 g/mL, respectivamente. A taxa total de recuperação das membranas analisados apresentaram valores entre 100,4 e 83,2%.Palavras-chave: ácido úsnico, membranas de colágeno, espectrofotometria UV The usnic acid (UA) is a secondary metabolite of lichen that has shown potential pharmacological effects such as antimicrobial, antiviral, antiproliferative and anti-inflammatory activities. Collagen is one of the most useful biomaterials with various applications as drug delivery systems. The aim of this study was to develop and validate a quantitative UV spectrophotometric method for the determination of UA levels in collagen membranes. The validation parameters were assessed according to the Brazilian Health Surveillance Agency (ANVISA). The specificity revealed that the excipients in the formulation did not interfere with the analysis. The linearity in the range of 2-10 μg/mL presented a correlation coefficient of 0.9994. The method showed excellent repeatability (Relative Standard Deviation (RSD) < 1.0%). The accuracy revealed a mean percentage recovery of 100.43%. The method was robust for the variation of temperature and solvent. The detection and quantization limits were found at 0.109 and 0.364 µg/mL. The total rate recovery from the analyzed membranes showed values between 100.4 and 83.2%.

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Evaluation of the Effects of Polarized Light (λ400–200 nm) on the Healing of Third-Degree Burns in Induced Diabetic and Nondiabetic Rats

Oliveira

Pinheiro²,

Castro³

et al. 2011

Photomedicine and Laser Surgery

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Análise histológica e morfométrica da área cruenta tratada com membrana de silicone: estudo em ratos

Abstract: The silicone membrane promoted granulation tissue with a large number of neovessels, fibroblasts and greater thickness.

Cited by 4 publications

References 18 publications

Evaluation of wound healing treated with latex derived from rubber trees and Aloe Vera extract in rats

Evaluation of wound healing treated with latex derived from rubber trees and Aloe Vera extract in rats

Validation of a UV-VIS Spectrophotometric method for the determination of usnic acid /collagen-based membranes

Evaluation of the Effects of Polarized Light (λ400–200 nm) on the Healing of Third-Degree Burns in Induced Diabetic and Nondiabetic Rats

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