Diarréia aguda grave associada à Escherichia coli enteropatogênica clássica (EPEC): características clínicas e perdas fecais em lactentes hospitalizados

Oliva, Carlos Alberto Garcia; Scaletsky, Isabel C. A.; Morais, Mauro Batista de; Neto, Ulysses Fagundes

doi:10.1590/s0104-42301997000400003

Cited by 3 publications

(1 citation statement)

References 17 publications

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“…Escherichia coli é uma bactéria gram-negativa não esporulada, possui forma de bastonete e classificada como um membro da família Enterobacteriaceae dentro da classe Gammaproteobacteria, apresenta colônias relativamente grandes, cinza-escuras úmidas ou mucóide (OLIVA et al, 1997;TENAILLON et al, 2016). Sua forma de locomoção se dá por meio de flagelos peritríquios ou não móveis, com tamanho aproximado de 2.0 µm de comprimento e 0.25-1.0 µm de diâmetro.…”

Section: Escherichia Coliunclassified

Incerteza de medição associada à contagem microbiana de produtos farmacêuticos

Dias¹

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Microbial contamination can compromise the efficacy and safety of pharmaceutical products.Microbial counting tests are used to assess the microbiological quality of non-sterile pharmaceutical products required by most pharmacopoeia compendiums. Despite this, measurement uncertainty assessment for microbial count tests is rarely considered, which can lead to false compliance/non-compliance decisions. In this work we evaluated the matrix effects on microbial counting tests and their top-down uncertainty assessment, and evaluated measurement uncertainty using the bottom-up approach, inaddition to estimating the consumer's or producer's risks due to measurement uncertainty. The combined and expanded uncertainties calculated using the top-down approach considered accuracy (recovery) and accuracy as the main components of uncertainty. The uncertainty component of accuracy was the most relevant in 59% of the samples studied, while accuracy was the main source of uncertainty in only 41% of the samples, being observed that the greater the interference of the matrix, the greater the uncertainty factor and, consequently, the greater the asymmetry for the interval around the measurement. From the bottom-up approach, three main sources of uncertainty were identified and quantified: dilution factor, platelet volume and plaque count. The contribution of these sources of uncertainty depends on the measured value of microbial load in pharmaceutical products, the contribution of the dilution factor and uncertainties of the plated volume increase with the increase in the measured value, while the contribution of plate counting decreases with the increase of the measured value.It was possible to assess the risk of false decisions due to measurement uncertainty by estimating consumer or producer risks. The risks were evaluated using the Monte Carlo method. Therefore, the relevance of measuring uncertainty assessment has been demonstrated to ensure the reliability of microbial count test results and to support decision-making when assessing non-sterile pharmaceutical conformity/non-compliance.

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Section: Escherichia Coliunclassified

Incerteza de medição associada à contagem microbiana de produtos farmacêuticos

Dias¹

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Enteropathogenic Escherichia coli-induced macrophage inhibitory cytokine 1 mediates cancer cell survival: an in vitro implication of infection-linked tumor dissemination

Choi

et al. 2013

Oncogene

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Mucosally adherent Escherichia coli is frequently observed in intestinal surface of patients with colorectal cancer, but rarely in healthy control. Particularly, enteropathogenic Escherichia coli (EPEC) is known to be closely associated with colorectal carcinogenesis in human. In this study, one consequence of EPEC infection in human intestinal cancer cells was induction of macrophage inhibitory cytokine 1 (MIC-1), which is a multifunctional cytokine with biological activities involved in cancer cell growth, differentiation and migration. The present investigation assessed the involvement of MIC-1 protein in EPEC infection-mediated cancer cell survival. The challenge with EPEC induced cancer cell detachment via cytoskeleton rearrangement, which was positively associated with induced MIC-1 expression. Moreover, MIC-1 also mediated RhoA GTPase-linked survival of the detached cancer cells. Blocking of MIC-1 or RhoA activity increased cellular apoptosis of the detached cancer cells. In terms of signaling pathway, MIC-1 triggered transforming growth factorβ-activated kinase 1 (TAK1), which enhanced expression of RhoA GTPase. We conclude that EPEC enhances MIC-1 gene expression in the human intestinal cancer cells, which can be associated with enhanced tumor cell resistance to anchorage-dependent tumor cell death via enhanced TAK1 and RhoA GTPase.

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Pesquisa de coliformes totais, fecais e Salmonella spp em tilápias de pesqueiros da região metropolitana de São Paulo

Liuson¹

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Diarréia aguda grave associada à Escherichia coli enteropatogênica clássica (EPEC): características clínicas e perdas fecais em lactentes hospitalizados

Cited by 3 publications

References 17 publications

Incerteza de medição associada à contagem microbiana de produtos farmacêuticos

Incerteza de medição associada à contagem microbiana de produtos farmacêuticos

Enteropathogenic Escherichia coli-induced macrophage inhibitory cytokine 1 mediates cancer cell survival: an in vitro implication of infection-linked tumor dissemination

Pesquisa de coliformes totais, fecais e Salmonella spp em tilápias de pesqueiros da região metropolitana de São Paulo

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