Evaluation of Escherichia coli cells damages induced by ultraviolet and proton beam radiation

Abstract

Prokaryote cells were exposed to ultra violet (UVc) radiation and to proton beams in order for the induced effects to be studied. Morphological and physiological alterations occurred in Escherichia coli (E. coli) cells exposed to the beams were investigated. The measurements using UVc radiation were made at the Biology Department of CEFET-PR while the measurements using proton beams were made at the Pelletron Accelerator of the Physics of the University at São Paulo. An exposition time of 3 to 15 seconds for UVc radiation and dose ranging from 0.2 to 10.0Gy for protons was used. A cellular survival curve versus exposition time and absorbed dose was built for each case. After the irradiation the cells were submitted to a series of biochemical tests. It was observed that the E.Coli cells lost some basic biochemical properties when the received doses were in the range of 0.2 to 0.7Gy. By microscopic observations it was noticed that the E.Coli cells elongated after irradiation with UVc as well as with proton beam.

Study of the effects of high-energy proton beams on escherichia coli

“…On the other hand, the survival curve of the E. coli exposed to doses ranging from 1 to 5 Gy of protons showed no significant decrease [10]. While the survival fractions were approximately 0.57, 0.26 and 0.004 at dose of 13, 46 and 93 Gy, respectively.…”

Proteomic Profiling of Escherichia coli in Response to Carbamate Pesticide - Methomyl

“…Prokaryotic cells respond to environmental or chemical stress by inducing specific sites of proteins characteristic to each stress 8 . Studies on stress response and survival strategies of enteric bacteria have evolved a range of complex mechanisms, which use different regulatory structures and genetic components for their survival and virulence 9 . The stress protein induced in response to four different pesticides viz.…”

Detection of Gamma Radiation Effect Induced by Cobelt-60 on Escherichia Coli Cells

“…The prokaryotes are interesting group of microorganisms. They possess intrinsic properties, such as reduced generation time and low cost of culture and maintenance so can be used as a tool for the scientific investigations to obtain important parameters for metabolic and genetic characterization of cells of more complex organisms (1), for example, using of bacterial cells as biosensor to sense the effect of ionizing radiation biologically, such as Escherichia coli (a common bacterium of the intestinal tract of human and animals) (2).…”