Detection of Gamma Radiation Effect Induced by Cobelt-60 on Escherichia Coli Cells

Abstract

Effect of ionizing radiation induced by coblet-60 (Co-60) on Escherichia coli cells was investigated. A cellular survival curve and mutant frequencies versus absorbed doses were determined. The results showed that the level of bacteria in survival curve decrease to (2) Log with gamma ray doses increase and D-value was equals (0.079) Gy. After irradiation the mutant frequencies increased with doses increase, the greatest mutant frequency was 3.4 10 -12 (1 mutation for 0.29 10 12 CFU for 150 g of rifampicin) induced by(1) Gy, while the smaller was 0.39 10 -12 (1 mutation for 3.41 10 12 CFU for 150 g of rifampicin) induced by(0.1) Gy.

Effect of Gama Irradiation of Bioethanol Producing Microorganisms on Bioethanol Formation from Sugarcane Bagasse and Potato Peels

“…The biological effects of ionizing radiation on cells is due to both direct interactions with critical cell components and indirect actions on these targets by molecular entities formed because of the radiolysis of other molecules in the cell, particularly by radicals formed from water. Ionizing radiation is capable of causing a variety of chemical changes in microorganisms, of which DNA is the most critical target of ionizing radiation (Al-Sudany et al, 2010 andGrecz et al, 1983). The low doses of gamma irradiation may enhance the activity of microorganisms in biological processes.…”