Genetic similarity among strawberry cultivars assessed by RAPD and ISSR markers

Morales, Rafael Gustavo Ferreira; Resende, Juliano Tadeu Vilela de; Faria, Marcos Ventura; Andrade, Marcela Carvalho; Resende, Luciane Vilela; Delatorre, Carla Andréa; Silva, Paulo Roberto da

doi:10.1590/s0103-90162011000600010

Cited by 36 publications

(26 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Quantitative agronomic traits are phenotypic markers widely used in genetic divergence studies (Morales et al, 2011a) with the advantage of being directly linked to the coding regions of the genome aside from the low acquisition cost (Gonçalves et al, 2008). However, environmental influences and the need to evaluate some variables in the adult plant stage are the main disadvantages of this marker group, hampering their use.…”

Section: Introductionmentioning

confidence: 99%

Prediction of industrial tomato hybrids from agronomic traits and ISSR molecular markers

Figueiredo¹,

Resende²,

Faria³

et al. 2016

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. Heterosis is a highly relevant phenomenon in plant breeding. This condition is usually established in hybrids derived from crosses of highly divergent parents. The success of a breeder in obtaining heterosis is directly related to the correct identification of genetically contrasting parents. Currently, the diallel cross is the most commonly used methodology to detect contrasting parents; however, it is a time-and cost-consuming procedure. Therefore, new tools capable of performing this task quickly and accurately are required. Thus, the purpose of this study was to estimate the genetic divergence in industrial tomato lines, based on agronomic traits, and to compare with estimates obtained using inter-simple sequence repeat (ISSR) molecular markers. The genetic divergence among 10 industrial tomato lines, based on nine morphological characters and 12 ISSR primers was analyzed. For data analysis, Pearson and Spearman correlation coefficients were calculated between the genetic dissimilarity measures estimated by Mahalanobis distance and Jaccard's coefficient of genetic dissimilarity from the heterosis estimates, combining ability, and means of important traits of industrial tomato. The ISSR markers efficiently detected contrasting parents for hybrid production in tomato. Parent RVTD-08 was indicated as the most divergent, both by molecular and morphological markers, that positively contributed to increased heterosis and by the specific combining ability in the crosses in which it participated. The genetic dissimilarity estimated by ISSR molecular markers aided the identification of the best hybrids of the experiment in terms of total fruit yield, pulp yield, and soluble solids content.

show abstract

Section: Introductionmentioning

confidence: 99%

Prediction of industrial tomato hybrids from agronomic traits and ISSR molecular markers

Figueiredo¹,

Resende²,

Faria³

et al. 2016

Genet. Mol. Res.

View full text Add to dashboard Cite

show abstract

“…However, Morales et al (2011) and Kalpana et al (2012) verified that ISSR markers were more polymorphic than the RAPD markers in strawberry and mulberry, respectively.…”

Section: Resultsmentioning

confidence: 93%

“…The combined use of RAPD and ISSR markers was successfully used in a considerable number of horticultural species such as potato (Moulin et al 2012), strawberry (Morales et al 2011) and mulberry (Awasthi et al 2004;Kalpana et al 2012).…”

Section: Resultsmentioning

confidence: 99%

Fingerprinting of Vaccinium corymbosum cultivars using DNA of fruits

Carvalho

Matos

Carnide

2014

Hort. Sci. (Prague)

View full text Add to dashboard Cite

Carvalho M., Matos M., Carnide V., 2014. Fingerprinting of Vaccinium corymbosum cultivars using DNA of fruits. Hort. Sci. (Prague), 41: 175-184.In recent years the production and consumption Vaccinium corymbosum has increased. Highbush blueberry cultivars are divided into three types, northern, intermediate and southern. The traditional methods for classification of highbush blueberry cultivars using morphological and flavour traits are largely unsuccessful, due to environmental influences. The genetic similarity of ten highbush blueberry cultivars was evaluated using random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers from fruits and leaves. The DNA concentrations obtained in fruits and leaves were very similar and the band profiles observed in the two tissues were analogous with both molecular markers. RAPD analysis generated 144 bands, of which 112 were polymorphic (77.8%) in fruits and 141 bands of which 118 were polymorphic (83.7%) in leaves. In fruits, ISSR analysis produced 151 bands of which 127 were polymorphic (84.1%) and in leaves it produced 148 bands with 127 being polymorphic (85.8%). Dendrogram and principal coordinates analysis (PCO) analysis using the both markers results were concordant and a clear division of the types of highbush blueberry cultivars (northern and southern) into two distinct groups was verified.

show abstract

“…This is due to the determination of the level of genetic divergence and the molecular pattern of each cultivar. With these data, the breeder can plan crosses using molecular and field data, which can result in the development of superior cultivars more quickly and economically (Langridge and Chalmers, 2004;Morales et al, 2011). Molecular markers are increasingly used in breeding programs of many horticultural crops.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity and chemical characterization of selected Polish and Russian cultivars and clones of blue honeysuckle (Lonicera caerulea)

Kaczmarska¹,

Gawroński²,

Dyduch‐Siemińska³

et al. 2015

Turk J Agric For

View full text Add to dashboard Cite

Positive properties of haskap berries, belonging to L. caerulea, have been known for centuries. From ancient times in Japan, honeysuckle has been used to treat urinary disorders, fever, and headache, and in Korea it was used widely for upper respiratory tract infections, diabetes mellitus, and rheumatoid arthritis (Skupień et al., 2007). The fruits are rich in ascorbic acid, macroand microelements, polyphenolic compounds (especially phenolic acids), anthocyanins, proanthocyanidins, flavonoids, and other bioactive substances. The major anthocyanins are glucosides and rutinosides (Frejnagel, 2007; Rop et al., 2011;Jurikova et al., 2012). The studies presented by Frejnagel ( 2007), Małodobry et al. (2010), andOchmian et al. (2012) indicated that haskap berries exhibit antifungal and anticancer properties, antiadherence, and chemoprotective effects. Compounds contained in the fruits provide protection from cancer, tumor growth, diabetes mellitus, and cardiovascular diseases, reducing blood pressure, decreasing the risk of heart attack, preventing osteoporosis and anemia, and slowing the aging process. According to Hoppula and Karhu (2006), the biochemical composition of the fruit is strongly affected by the genotype, harvest date, and environmental conditions. Total antioxidant activity, as well as phenolic acid and flavonoid content, can vary among cultivars. There is a pressing need to develop reliable methods for identifying blue honeysuckle cultivars/clones and for assessing genetic diversity in Lonicera genotypes for practical breeding purposes. To start a breeding program it is essential to characterize the available germplasm, either morphologically or molecularly. Germplasm characterization by DNA-based molecular markers allows a better understanding of genetic variability. This is due to the determination of the level of genetic divergence and the molecular pattern of each cultivar. With these data, the breeder can plan crosses using molecular and field data, which can result in the development of superior cultivars more quickly and economically (Langridge and Chalmers, 2004;Morales et al., 2011). Molecular

show abstract

Genetic similarity among strawberry cultivars assessed by RAPD and ISSR markers

Cited by 36 publications

References 10 publications

Prediction of industrial tomato hybrids from agronomic traits and ISSR molecular markers

Prediction of industrial tomato hybrids from agronomic traits and ISSR molecular markers

Fingerprinting of Vaccinium corymbosum cultivars using DNA of fruits

Genetic diversity and chemical characterization of selected Polish and Russian cultivars and clones of blue honeysuckle (Lonicera caerulea)

Contact Info

Product

Resources

About