Low carbon/nitrogen ratio increases laccase production from basidiomycetes in solid substrate cultivation

D'Agostini, Érica Clarissa; Mantovani, Talita Rafaele D’Agostini; Valle, Juliana Silveira do; Paccola-Meirelles, Luzia Doretto; Colauto, Nelson Barros; Linde, Giani Andréa

doi:10.1590/s0103-90162011000300004

Cited by 48 publications

(26 citation statements)

References 31 publications

(35 reference statements)

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“…Laccase production by Lentinus crinitus increases in the presence of 2.8 g/L nitrogen (Valle et al, 2014b) while that by P. ostreatus increases in the presence of 0.23 g/L nitrogen (Hou et al, 2004). The results of the present study and those of other studies (Ullrich et al, 2005;D'Agostini et al, 2011;Valle et al, 2014a) suggest that nitrogen concentrations in the range of 1.5-3.0 g/L induce maximum laccase production by A. blazei. Further, use of a protein nitrogen source such as urea promotes laccase production while use of inorganic nitrogen decreases laccase production (Piscitelli et al, 2011).…”

Section: Effect Of Nitrogen and Copper Concentrations On Laccase And supporting

confidence: 64%

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Effect of different compounds on the induction of laccase production by Agaricus blazei

Valle¹,

Vandenberghe²,

Oliveira³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Laccases are polyphenol oxidases produced by many fungi and have many applications in textile, food and beverage, and pulp and paper industries. Laccase production can be induced using aromatic or phenolic compounds that mostly affect the transcription of laccase-encoding genes. In this study, we analyzed laccase and biomass production by Agaricus blazei in the presence of different concentrations of nitrogen, copper, and inducers such as pyrogallol, veratryl alcohol, xylidine, vanillin, guaiacol, and ethanol. Laccase production by A. blazei U2-4 reached 43.8 U/mL in the presence of 2.8 g/L nitrogen and 150 µM copper. However, addition of copper to the cultivation medium decreased biomass production. Different compounds differentially induced laccase production by A. blazei. Moreover, different concentrations of these inducers exerted different effects on laccase activity. Ethanol (1.0 mM), guaiacol (0.5 mM), and vanillin (0.5 mM) were the best inducers and increased laccase activity by 120% (A. blazei U2-2), 30% (A. blazei U2-3), and 9% (A. blazei U2-4), 15883Induction of laccase production by Agaricus blazei ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (4): 15882-15891 (2015) respectively. In contrast, pyrogallol and xylidine decreased laccase activity but increased biomass production.

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Section: Effect Of Nitrogen and Copper Concentrations On Laccase And supporting

confidence: 64%

“…It degrades lignin and structurally similar compounds at a lower rate than white-rot fungi. A. blazei is a laccase producer (Ullrich et al, 2005;D'Agostini et al, 2011;Valle et al, 2014); however, no information is available on compounds that induce laccase production by A. blazei.…”

Section: Introductionmentioning

confidence: 99%

Effect of different compounds on the induction of laccase production by Agaricus blazei

Valle¹,

Vandenberghe²,

Oliveira³

et al. 2015

Genet. Mol. Res.

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“…As far as the effect of AN on the decomposition of lignin is concerned, in the study of Allison et al [29] there was no significant effect for both ratios tested compared to the control, which is consistent with the findings of the present study. D'Agostini et al [30] have examined the effect of different C/N ratios (5-30, corresponding to~100 mg·N/g·C-16 mg·N/g·C) in lacasse production, during growth of three different basidiomycetes, Pleurotus ostreatus, Lentinula edodes, and Agaricus blazei on soybean hulls, by adding N in the form of AN and/or UR. It was shown that higher C/N ratios, i.e., lower N/C ratios, increased mycelial growth, but decreased laccase production.…”

Section: Effect Of Nitrogen Supplementation On the Lignocellulosic Comentioning

confidence: 99%

Fungal Pretreatment of Willow Sawdust with Abortiporus biennis for Anaerobic Digestion: Impact of an External Nitrogen Source

et al. 2017

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Abstract:In this study the effect of nitrogen (N) supplementation in the fungal pretreatment of willow sawdust (WSD) via the white rot fungus Abortiporus biennis (A. biennis) was studied in terms of the fractionation of lignocellulosic biomass and biochemical methane potential (BMP). Thus, different external nitrogen sources (yeast extract (YE), urea (UR), and ammonium nitrate (AN)) at different ratios (N/C of 1/250 and 1/50) were added and the effect of the above parameters on the chemical composition of WSD during solid-state fermentation (SSF) experiments with A. biennis was assessed and compared to the experiment of fungal pretreatment without N supplementation (N/C was 1/500, control experiment). The results indicated that the addition of an external nitrogen source did not facilitate delignification, regardless of the type of nitrogen source and the ratio of N/C used. On the other hand, enhanced cellulose uptake was observed. Samples of the 28th day of cultivation, with and without N supplementation, were used for BMP tests, where a reduction in methane yield was observed, compared to the control experiment. In addition, a combination of fungal with alkali (20% NaOH w/w dry mass) pretreatment was performed in order to assess the effect of combined pretreatment on the lignocellulosic content and the BMP.

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“…However, the initial rate of decolorization was 1.6-fold as compared to medium 3 (6.71 mg/L h). It has been shown that decolorizing and biodegradation activities of various basidiomycetes are higher in lownutrient media due to the induction of some enzymes that contribute to the breakdown of substrates, releasing carbon and nitrogen resources (D'Agostini et al 2011;Hailei et al 2009;Iqbal et al 2011;Yamanaka et al 2008). Considering the high initial decolorizing rate of T. polyzona LMB-TM5, the indifference of Ceriporia sp.…”

Section: Developing Fungal Biofilmsmentioning

confidence: 99%

Decolorization of Textile Reactive Dyes and Effluents by Biofilms of Trametes polyzona LMB-TM5 and Ceriporia sp. LMB-TM1 Isolated from the Peruvian Rainforest

Cerrón

Romero-Suárez

Vera

et al. 2015

Water Air Soil Pollut

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The textile industry creates environmental problems due to the release of highly polluting effluents containing substances from different stages of dyeing that are resistant to light, water, and various chemicals, and most of them are difficult to decolorize because of its synthetic origin. The biological degradation of dyes is an economical and environmentally friendly alternative. The aim of this work was to use biofilms of basidiomycete fungi isolated from the Peruvian rainforest for the decolorization of synthetic reactive dyes, considering the advantages of these systems which include better contact with the surrounding medium, resistance to chemical and physical stress, and higher metabolic activity. Among several isolates, two were selected for their capacity of rapid decolorization of several dyes and their biofilm-forming ability. These strains were molecularly identified as Trametes polyzona LMB-TM5 and Ceriporia sp. LMB-TM1 and used in biofilm cultivation for the decolorization of six reactive dyes and textile effluents. Azo dyes were moderately decolorized by both strains, but Remazol Brilliant Blue R (anthraquinone) and Synozol Turquoise Blue HF-G (phthalocyanine) were highly decolorized (97 and 80 %, respectively) by T. polyzona LMB-TM5. Degradation products were found by HPLC analysis. Simulated effluents made of a mixture of six dyes were moderately decolorized by both strains, but a real textile effluent was highly (93 %) decolorized by T. polyzona LMB-TM5. In summary, T. polyzona LMB-TM5 was more efficient than Ceriporia sp. LMB-TM1 for the decolorization of textile dyes and effluents at high initial rates enabling the development of in-plant continuous biofilm processes.

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Low carbon/nitrogen ratio increases laccase production from basidiomycetes in solid substrate cultivation

Cited by 48 publications

References 31 publications

Effect of different compounds on the induction of laccase production by Agaricus blazei

Effect of different compounds on the induction of laccase production by Agaricus blazei

Fungal Pretreatment of Willow Sawdust with Abortiporus biennis for Anaerobic Digestion: Impact of an External Nitrogen Source

Decolorization of Textile Reactive Dyes and Effluents by Biofilms of Trametes polyzona LMB-TM5 and Ceriporia sp. LMB-TM1 Isolated from the Peruvian Rainforest

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