Effect of different compounds on the induction of laccase production by Agaricus blazei

Valle, Juliana Silveira do; Vandenberghe, Luciana Porto de Souza; Oliveira, Ana Carolina Costa de; Tavares, Magali Ferreira; Linde, Giani Andréa; Colauto, Nelson Barros; Soccol, Carlos Ricardo

doi:10.4238/2015.december.1.40

Cited by 31 publications

(24 citation statements)

References 28 publications

(41 reference statements)

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“…The enzymatic activity was determined in a reaction mixture containing 0.2 mL cultivation medium, 0.7 mL ultrapure water, 0.45 mL sodium acetate buffer (0.1 M, pH 5.0), and 0.15 mL ABTS (Valle et al, 2015). The reaction mixture was maintained at 30°C for 10 min and then 100 mL trichloroacetic acid solution (50 g/L) was added to the enzymatic reaction.…”

Section: Laccase Assaymentioning

confidence: 99%

“…This species is found in tropical and subtropical areas and is known for its high lignocellulolytic potential, for producing laccase as its main extracellular ligninase (Hernández et al, 2016), and for effectively degrading several classes of dyes (Moreira-Neto et al, 2013). However, laccase production depends on fungal cultivation and growth conditions, and the expression of different laccase isozymes is related to the presence of nutrients and inducers in the cultivation media (Valle et al, 2015). Different laccase isoforms may have distinct roles in fungal physiology and could be used for applications other than those already known.…”

Section: Introductionmentioning

confidence: 99%

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Use of sugarcane molasses by Pycnoporus sanguineus for the production of laccase for dye decolorization

Marim¹,

Oliveira²,

Marquezoni³

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Pycnoporus sanguineus is a white-rot basidiomycete that produces laccase as the only oxidoreductase; enzyme synthesis depends on cultivation variables, and fungal species and strain. Laccases have wide substrate specificity, oxidize a broad range of compounds, and show potential for use in dye decolorization. We evaluated laccase production in a recently isolated strain of P. sanguineus cultivated with sugarcane molasses as the only carbon source, and urea or yeast urea) increased enzymatic activity. The addition of up to 300 µM of Cu did not affect laccase production, whereas cultivation with agitation increased the activity peak by 17%. The highest laccase activity was ~50,000 U/L on the ninth day of cultivation. After 24 h, decolorization was 80% for Remazol Brilliant Blue R, 9% for Reactive Yellow 145, 6% for Reactive Red 195, and 2% for Reactive Black 5. The enzymatic extract of P. sanguineus provides a potential alternative to wastewater treatment. A better understanding of the behavior of this fungus under various culture conditions would allow improvement of the enzyme production bioprocess.

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Section: Laccase Assaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Use of sugarcane molasses by Pycnoporus sanguineus for the production of laccase for dye decolorization

Marim¹,

Oliveira²,

Marquezoni³

et al. 2016

Genet. Mol. Res.

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“…Two strains of L. crinitus (U9-1 and U15-9) belonging to the culture collection of the Graduate Program of Biotechnology Applied to Agriculture of Paranaense University were utilized. The identification of strains was done by sequencing the internal transcribed spacers (ITS) of ribosomal DNA (Valle et al, 2015). The DNA was sequenced using ABI Prism 3100 Genetic Analyzer (Applied Biosystems, Carlsbad, CA, USA).…”

Section: Microorganism and Inoculummentioning

confidence: 99%

“…Laccase production was evaluated using 1 mM ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) as substrate. The enzymatic activity was determined in a reaction containing 200 µL cultivation medium, 700 µL water, 450 µL sodium acetate buffer (0.1 M, pH 5.0) and 150 µL ABTS (Valle et al, 2015). The reaction mixture was maintained at 30°C for 10 min, and 100 µL trichloroacetic acid solution (50 g/L) was added to the reaction to interrupt enzymatic activity.…”

Section: Laccase Assaymentioning

confidence: 99%

Research Article Lentinus crinitus strains respond differently to cultivation pH and temperature

Marim¹,

Avelino²,

Linde³

et al. 2018

Genet. Mol. Res.

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Fungus ability to respond to environmental changes allows their adaptation to a wide variety of conditions such as pH, temperature, light, nutrient availability, chemicals, and competition among species. Temperature and pH can affect fungal growth as well as their metabolism. Knowing these factors on fungus species is relevant for the development of biotechnological applications and process optimization to produce biomass and enzymes. This study aimed to evaluate Lentinus crinitus mycelial growth and laccase production at different initial pH and temperatures of the cultivation medium. L. crinitus U15-9 reduced laccase production with pH increase with maximum production at pH 6 (39 U/mL) whereas L. crinitus U9-1 increased laccase production with pH increase with maximum activity at pH 7 (25.8 U/mL). Neither of the strains had mycelial growth significantly affected by initial pH in the culture medium. The greatest laccase production occurred at 28°C for U15-9 (50 U/mL) and U9-1 (41.5 U/mL). An increase in U9-1 mycelial biomass occurred when the temperature went up from 25 to 28°C whereas for U15-9 it was from 28 to 37°C; at 37°C, U15-9 produced 225% more mycelial biomass than at 28°C, and 108% more mycelial biomass than U9-1 at 37°C. The variation of responses to environmental stimuli between the strains makes evident that intra-specific variations are common in basidiomycetes. Our findings highlighted the best conditions of pH and temperature to produce L. crinitus laccase and make evident how different strains respond distinctly to cultivation conditions.

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“…Entretanto, a produção de lacase por fungos depende das condições de cultivo e está relacionada com a presença de nutrientes e indutores no meio de cultivo (VALLE et al, 2015). Isoformas distintas de lacases podem apresentar diferentes papéis na fisiologia do fungo e poderiam ser utilizadas em grande variedade de aplicações (MARIM et al, 2016).…”

Section: Introductionunclassified

PRODUÇÃO DE LACASE DE Pycnoporus sanguineus EM MEIO DE CULTIVO À BASE DE MELAÇO SOJA

Fabrini

Avelino

Marim

et al. 2017

ArqVet

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Umuarama, v. 19, n. 3, p. 159-164, jul./set. 2016. RESUMO:Lacases são polifenol oxidases que utilizam a capacidade redox de íons cobre para reduzir oxigênio a água e oxidar um substrato fenólico. A síntese e secreção de lacases de basidiomicetos dependem de vários fatores como os nutrientes presentes no meio de cultura. Visando à produção de lacase, Pycnoporus sanguineus foi cultivado em meio contendo melaço de soja como única fonte de carbono, ureia como fonte de nitrogênio suplementar em diferentes concentrações (0,6; 1,2; 2,4; 4,8 e 9,6 g/L de nitrogênio) e diferentes concentrações de CuSO 4 (0, 150, 200, 250 e 300 µM). O extrato enzimático produzido nas melhores condições de cultivo foi utilizado para a descoloração dos corantes remazol azul brilhante R (antraquinona), amarelo 145, preto 5, vermelho 195 (azo) e verde malaquita (trifenilmetano). As concentrações de nitrogênio não afetaram a produção de lacase, exceto a maior concentração (9,6 g/L) que reduziu a atividade enzimática. A adição de cobre ao meio de cultivo (150 µM) aumentou a atividade de lacase em 112%. A maior atividade de lacase (~34300 U/L) promoveu a descoloração dos corantes remazol azul brilhante R (67,5%) e verde malaquita (28,3%) em 24h, sendo os corantes azo descoloridos apenas parcialmente. Concluiu-se que o melaço de soja é um resíduo agroindustrial adequado para produção de lacase de P. sanguineus com potencial para degradação de corantes. PALAVRAS-CHAVE: Antraquinona. Corante azo. Ligninases. Poliporaceae. Resíduos agroindustriais. LACCASE PRODUCTION BY Pycnoporus sanguineus IN CULTURE MEDIA WITH SOYBEAN MOLASSESABSTRACT: Laccases are multicopper oxidases using the redox ability from copper ions to reduce oxygen to water, while oxidizing a phenolic substrate. Laccase synthesis and secretion in basidiomycetes depend on the conditions provided and on the nutrients present in the culture medium. Pycnoporus sanguineus was cultivated in medium containing soybean molasses as the sole carbon source, with urea as the source of supplemental nitrogen at different concentrations (0.6, 1.2, 2.4, 4.8 and 9.6 g/L nitrogen), and different CuSO 4 concentrations (0, 150, 200, 250 and 300 μM). The enzymatic extract produced under the best cultivation conditions was used for the depigmentation of remazole brilliant blue R (anthraquinone), yellow 145, black 5, red 195 (azo) and malachite green (triphenylmethane). Nitrogen concentrations did not affect laccase production, except for the higher concentration (9.6 g/L) reducing enzymatic activity. The addition of copper to the culture medium (150 μM) increased laccase activity by 112%. The highest laccase activity (~34300 U/L) promoted the depigmentation of remazol brilliant blue R (67.5%) and malachite green (28.3%) dyes in 24 hours. Azo dyes were only partially discolored. Therefore, it can be considered that soybean molasses is an agro-industrial byproduct suitable for the production of P. sanguineus laccase with potential for dye degradation. KEYWORDS: Agro-industrial byproduct. Anthraquinone. Azo d...

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Effect of different compounds on the induction of laccase production by Agaricus blazei

Cited by 31 publications

References 28 publications

Use of sugarcane molasses by Pycnoporus sanguineus for the production of laccase for dye decolorization

Use of sugarcane molasses by Pycnoporus sanguineus for the production of laccase for dye decolorization

Research Article Lentinus crinitus strains respond differently to cultivation pH and temperature

PRODUÇÃO DE LACASE DE Pycnoporus sanguineus EM MEIO DE CULTIVO À BASE DE MELAÇO SOJA

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