Antifungal compound produced by the cassava endophyte Bacillus pumilus MAIIIM4a

Abstract

In the search for new organisms and new secondary metabolites, a study was conducted to evaluate the diversity of endophytic bacteria from ethnovarieties of cassava cultivated by Brazilian Amazon Indian tribes and also to study the secondary metabolites produced by a Bacillus pumilus strain. Sixty seven cassava endophytic bacteria were subjected to 16S rRNA sequencing and FAME analysis. The bacterial profile revealed that 25% of all endophytic isolates belonged to the genus Bacillus. The isolate B. pumilus MAIIIM4a showed a strong inhibitory activity against the fungi Rhizoctonia solani, Pythium aphanidermatum and Sclerotium rolfsii. Secondary metabolites of this strain were extracted using hexane, dichloromethane and ethyl acetate. Extracts were subjected to bioautography and LC/MS analysis, which allowed the identification of pumilacidin, an antifungal compound produced by B. pumilus MAIIIM4a. The bacterial endophytic localization was confirmed by cassava cell tissue examination using scanning electron microscopy. Key words: ESI-MS/MS, pumilacidin, endophytic bacteria, bioautography COMPOSTO ANTIFÚNGICO PRODUZIDO PELO ENDÓFITO DE MANDIOCA Bacillus pumilus MAIIIM4ARESUMO: Na busca de novos organismos e novos metabólitos secundários, um estudo foi conduzido visando avaliar a diversidade de bactérias endofíticas de etnovariedades de mandioca cultivadas por tribos indígenas da Amazônia brasileira e também para estudar metabólitos secundários produzidos por Bacillus pumilus. Sessenta e sete bactérias endofíticas de mandioca foram identificadas através do seqüenciamento do gene 16S rRNA e por meio da análise de ácidos graxos (FAME

Pumilacidin-Like Lipopeptides Derived from Marine Bacterium Bacillus sp. Strain 176 Suppress the Motility of Vibrio alginolyticus

“…3C), differing in only the fatty acid chains (C 13 H 27 -and C 14 H 29 -) (Fig. 3C) with reported pumilacidins (34). Interestingly, the fatty acid moieties of the two lipopeptides were different in only one methylene group (CH 2 -) (Fig.…”

“…Among these lipopeptides, pumilacidin was a novel lipopeptide similar to surfactin and was first characterized from Bacillus pumilus (26). Although there have been only a few studies of pumilacidin after its discovery, it has been reported to possess different functions, such as antiviral (26), antifungal (34), and antibacterial (36) applications. To date, none of the lipopeptides have been reported to possess antimotility activity.…”

Isolation and Characterization of Antibacterial Compound from a Mangrove-Endophytic Fungus, Penicillium chrysogenum MTCC 5108

“…More than 200 species of endophytic fungi have been isolated and identified from mangrove plants and several reports on the isolation of antibiotic compounds from endophytic fungi have been reported. To mention a few griseofulvin, [7], chaetomugilin A and D [8], cytosporone B and C [9], 3-O-methylalaternin and altersolanol A [10], phomoenamide [11], phomodione [12], ambuic acid [13], isopestacin [14], munumbicin A, B, C, and D [15], pestalachlorides A and B, [16], brefeldin A [17], coronamycin, [18], pumilacidin [19], cytochalasin D [20], polyketide citrinin [21,22], fumigaclavine C, fumitremorgin C, physcion [23], and several others.…”

Screening of Endophyte Piper betle Bacteria from the Forests of HPPB University Andalas as Antibiotics Producer

“…Healthy leaf samples, not young leaves or old leaves, each taken ten pieces of betel leaf from each clump. Leaf collecting from plants refers to Melo et al, (2009) that has been modified, the leaves to be collected are cut with a sterile knife then washed with sterile distilled water, then the green betel leaf is placed in a plastic bag and placed in a cooler. Leaves of plants that have been collected immediately brought to the laboratory.…”