Practical method for germination of Araucaria angustifolia (Bert.) O. Ktze. seeds

Moreira-Souza, Milene; Cardoso, Elke Jurandy Bran Nogueira

doi:10.1590/s0103-90162003000200025

Cited by 21 publications

(13 citation statements)

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“…Seeds were surfacedecontaminated with sodium hypochlorite solution (2 %, v/v) for three minutes, and subsequently the tip of each seed was cut off at approximately 3 mm (Moreira-Souza & Cardoso, 2003). Four replications of 25 seeds each were sown in trays with vermiculite, and placed in a germination chamber at 25 °C, with photoperiod of 12 hours.…”

Section: Germination Testmentioning

confidence: 99%

Early harvest increases post-harvest physiological quality of Araucaria angustifolia (Araucariaceae) seeds

Shibata

Coelho²

2016

RBT

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Abstract:Araucaria angustifolia is a conifer native to Brazil and is an endangered species. Since this species seeds have a short period of viability, its vulnerability is higher. Thus the aim of this study was to evaluate the physiological quality of A. angustifolia seeds during the development and post-storage periods. For this, cones of A. angustifolia were collected from a natural population in Curitibanos, Santa Catarina, Brazil, in March, April, May and June 2012. The collected seeds were classified into developmental stages of cotyledonary, i, ii and iii according to the month of collection; a total of 10 cones were collected for each stage. Seeds were stored in a refrigerator for 60 and 120 days, and were submitted to a chamber germination test (25 °C-photoperiod 12 h). Additionally, seeds were tested for moisture content (105 °C for 24 hours), tetrazolium (0.1 % for 1 hour) and vigor (electric conductivity [75 mL distilled water at 25 °C], germination speed index, and shoot and root length). Our results showed that during seed development, moisture content decreased from the cotyledonary stage (66.54 %) to stage iii (49.69 %), and vigor increased in the last stage. During storage, moisture content at cotyledonary stage and stage i was stable. On the other hand, stored seeds exhibited a decrease in moisture content after 120 days at stages ii and iii. Physiological quality at the cotyledonary stage resulted in an increased germination rate of 86 % and 93 % after 60 and 120 days of storage, respectively; unlike stages ii and iii exhibited a decrease in seed viability and vigor after storage. Electrical conductivity was higher for fresh seeds at the cotyledonary stage, than for those stored for 60 and 120 days. However, in other stages, released leachate content after 120 days of storage, increased with the advance of the collection period. Germination speed index and shoot and root lengths after storage were highest for seeds at the cotyledonary stage and stage i; unlike stages ii and iii which had short root and shoot lengths during storage. Thus, the maintenance of seed moisture content during storage was variable and dependent on the period of collection. Furthermore, the physiological quality differed among earlier and later stages. Early collection favored seed physiological quality, and may be a strategy for better conservation of A. angustifolia seeds. Rev. Biol. Trop. 64 (2): 885-896. Epub 2016 June 01.Key words: recalcitrant seed, storage, seed development, gymnosperms, endangered plants.Seed development is a process that comprises a set of morphological, cellular, and biochemical/synthetic changes which commences with ovule fertilization to the moment in which the seed is mature (Bewley, Bradford, Hilhorst, & Nonogaki, 2013).Most seeds undergo a process of "maturation drying" with late stages of development, and these seeds are called orthodox (Roberts, 1973). Before or concomitant with maturation drying, seeds acquire desiccation tolerance, and can be dried to low water content (generally less ...

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Section: Germination Testmentioning

confidence: 99%

Early harvest increases post-harvest physiological quality of Araucaria angustifolia (Araucariaceae) seeds

Shibata

Coelho²

2016

RBT

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“…Plagiotropism in araucaria is also a great challenge that needs to be overcome (OLIVEIRA, 2010;WENDLING, 2011). For example, it has been observed that rooted cuttings of primary branches still had horizontal growth four years after planting (OLIVEIRA, 2010), preventing the development of normal plants.…”

Section: Figura 4 -Sobrevivência Na Saída Da Casa De Vegetação (A) Cmentioning

confidence: 99%

“…For example, it has been observed that rooted cuttings of primary branches still had horizontal growth four years after planting (OLIVEIRA, 2010), preventing the development of normal plants.…”

Section: Figura 4 -Sobrevivência Na Saída Da Casa De Vegetação (A) Cmentioning

confidence: 99%

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VEGETATIVE RESCUE AND CUTTINGS PROPAGATION OF Araucaria angustifolia (Bertol.) Kuntze

Wendling

Brondani

2015

Rev. Árvore

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-Brazilian pine or araucaria (Araucaria angustifolia) is a coniferous tree with great economic, social and environmental importance in southern Brazil, being exploited for both wood production and for its edible pine nuts. However, no efficient cloning techniques are available and, therefore, the purpose of this study was to evaluate the effectiveness of vegetative rescue methods for cuttings propagation of the species. Shoots/cuttings were generated in two ways: 26 years old trees underwent coppicing and 20 years old trees had the primary branches on the upper third of crown pruned at 2, 20 and 50 cm from the main trunk. Orthotropic shoots were rooted after application of indole-3-butyric acid (IBA) at 0, 2, 4 and 6 g.L -1. Coppicing produced 47 cuttings per plant with 90% orthotropic shoots, while pruning resulted in 182 cuttings per plant with 44% orthotropic shoots. Rooting success indexes were low with no influence of IBA, although they are slightly superior to the ones available in the literature for the species, ranging from 12 to 30% for the coppice shoots and from 0 to 28% for the branches shoots. We conclude that both vegetative rescue techniques are viable and have potentially important applications. Coppicing is recommended for the propagation aiming the production of wood, while shoots derived from the side branches of the crown are more appropriate for seeds orchards formation.Keywords: Brazilian pine; Cutting propagation; Clonal forestry. RESGATE VEGETATIVO E ESTAQUIA DE Araucaria angustifolia (Bertol.) Kuntze RESUMO -A araucária, ou pinheiro-brasileiro (Araucaria

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“…As avaliações de plântulas normais foram realizadas a cada três dias para o cálculo do índice de velocidade de germinação (IVG) até o período de 64 dias após o início do teste de germinação, respeitando-se a expressão do máximo potencial germinativo das sementes. Os períodos de 60 e 70 dias após o início do teste de germinação foram considerados por outros autores para a análise dos resultados de plântulas normais em sementes de A. angustifolia, quando há a máxima germinação (Moreira-Souza & Cardoso, 2003;Garcia et al, 2014, respectivamente). O critério para avaliação de plântulas normais foi adotado conforme prescrito nas RAS (Brasil, 2009).…”

Section: Introductionunclassified

pH do Exsudato na Avaliação da Viabilidade de Sementes de Araucaria angustifolia

Araldi

Coelho

2015

Floresta Ambient.

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RESUMOEste estudo objetivou verificar a eficiência de métodos de pré-condicionamento e períodos de embebição em água para a realização do teste de pH do exsudato para avaliação da viabilidade de sementes de Araucaria angustifolia. Testaram-se três pré-condicionamentos (semente inteira; semente seccionada longitudinalmente; embrião excisado) e três períodos de embebição em água (30, 60 e 90 minutos), avaliando-se a coloração da solução. Adicionalmente, as sementes foram envelhecidas artificialmente por 4 e 8 dias, a 40 °C. A viabilidade foi superior em embriões excisados e embebidos por 30 minutos, apresentando elevado coeficiente de correlação com os testes de germinação e tetrazólio. Para sementes em avançado estádio de deterioração, a avaliação deve associar a coloração da solução ao aspecto dos tecidos. Conclui-se que o teste do pH do exsudato é eficiente na avaliação da viabilidade de sementes de A. angustifolia, devendo ser realizado utilizando-se apenas o embrião embebido em água por 30 minutos.Palavras-chave: germinação, qualidade fisiológica, sementes florestais. pH Exudate Test for Determining the Viability of Araucaria angustifolia Seeds ABSTRACTThis study aimed to verify the efficiency of methods of pre-conditioning and periods of water immersion to perform the pH exudate test to evaluate the viability of Araucaria angustifolia seeds. Three pre-conditioning methods (whole seed, seed longitudinally sectioned, excised embryo) and three water imbibition periods (30, 60 and 90 minutes) were tested, and the solution color was evaluated. Additionally, the seeds were artificially aged for four and eight days at 40 °C. The viability was higher in embryos excised, after 30 minutes of imbibition, showing high coefficient of correlation with the germination and tetrazolium tests. Seeds at advanced stage of deterioration should be evaluated by associating the solution color with the tissue aspect.In conclusion, the pH exudate test is efficient to evaluate the viability of A. angustifolia seeds, and it should be performed using excised embryo imbibed in water for 30 minutes.

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Practical method for germination of Araucaria angustifolia (Bert.) O. Ktze. seeds

Cited by 21 publications

References 4 publications

Early harvest increases post-harvest physiological quality of Araucaria angustifolia (Araucariaceae) seeds

Early harvest increases post-harvest physiological quality of Araucaria angustifolia (Araucariaceae) seeds

VEGETATIVE RESCUE AND CUTTINGS PROPAGATION OF Araucaria angustifolia (Bertol.) Kuntze

pH do Exsudato na Avaliação da Viabilidade de Sementes de Araucaria angustifolia

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