Sementes de feijão submetidas a ciclos e períodos de hidratação-secagem

Aragão, Carlos Alberto; Dantas, B. F.; Alves, Elza; Corrêa, Marcelo Rocha

doi:10.1590/s0103-90162002000100013

Cited by 12 publications

(12 citation statements)

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“…In addition, PIII was characterised by the radicle protrusion and the resumption of the water uptake. However, unlike PI, this phase showed an active water absorption (BEWLEY et al, 2013). P. pyramidalis seeds presented a sharp increase in the water uptake, while S. obtusifolium seeds showed a smoother increase.…”

Section: Resultsmentioning

confidence: 87%

“…According to Bewley et al (2013), in the first hours of imbibition, a large amount of exudates (sugars, ions, amino acids and proteins) are leached into the surrounding medium. The content of albumins, enzymes, in general, and other water-soluble proteins decreased during PI, and these substances were produced again during PII (Figures 4A).…”

Section: Resultsmentioning

confidence: 99%

“…The residue obtained after the extraction of the albumin fraction similarly underwent the extraction of the globulin fraction using NaCl (1%) as solvent. Likewise, for the extraction of prolamins and glutelins, we used ethanol (70%) and NaOH (0.1 mol L -1 ), respectively (ARAGÃO et al, 2003). The proteins were quantified according to Bradford (1976) using bovine serum albumin (BSA) as a reference curve.…”

Section: Methodsmentioning

confidence: 99%

“…When germination begins, there is an activation of the protein synthesis and the formation of hydrolytic enzymes that promote the mobilisation of reserves, among which are the storage proteins. These proteins, in turn, form the basis of agriculture because of the variety of essential functions that they perform as food (CARVALHO;NAKAGAWA, 2012;BEWLEY et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…Albumins are water-soluble proteins, globulins are soluble in saline solutions, prolamins are soluble in hydroalcoholic solutions, and glutelins are soluble in diluted acidic or basic solutions. Most studies related to seed storage proteins involve crops such as legumes, cucurbits, brassicas, among others (BEWLEY et al, 2013). According to MataAtaíde et al (2014), information on the metabolic processes (physiological and biochemical) involved in germination are incipient for most native species, especially during water uptake by seeds.…”

Section: Introductionmentioning

confidence: 99%

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Quantification of storage proteins during seed imbibition of native species from the brazilian caatinga vegetation

Silva

Dantas

2016

SCA

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Poincianella pyramidalis (Fabaceae), Schinopsis brasiliensis (Anacardiaceae) and Sideroxylon obtusifolium (Sapotaceae) are native species of the Caatinga vegetation from northeastern Brazil and have both biological importance and potential economic uses. Little is known about the water uptake and degradation of storage proteins during seed germination of these species. The aim of this study was to evaluate the imbibition and quantify the amount of storage proteins during seed germination of P. pyramidalis, S. brasiliensis and S. obtusifolium. Two lots of S. obtusifolium seeds with different vigour were used. Four replicates of 20 seeds of P. pyramidalis, S. brasiliensis and S. obtusifolium, were sown onto gerboxes with blotting paper soaked in distilled water and incubated during 72, 200 and 624 hours. Before and after imbibition seeds were weighed and frozen at until the sequential extraction and analysis of the seed storage proteins. Based on our results, we conclude that seed germination of P. pyramidalis, S. brasiliensis and S. obtusifolium has a well-defined triphasic imbibition. All storage proteins content of P. pyramidalis and S. brasiliensis seeds degraded along with the seed imbibition. Likewise, the content of albumins, globulins and glutelins decreased as S. obtusifolium seeds absorbed water. Key words: Germination. Poincianella pyramidalis. Reserve mobilisation. Schinopsis brasiliensis. Sideroxylon obtusifolium. Tropical dry forest. ResumoPoincianella pyramidalis (Fabaceae), Schinopsis brasiliensis (Anacardiaceae) e Sideroxylon obtusifolium (Sapotaceae) são espécies nativas da Caatinga e que apresentam grande importância biológica e potencial econômico. Pouco se sabe sobre a absorção de água e degradação de proteínas de reserva durante a germinação dessas espécies. O objetivo deste trabalho foi avaliar a embebição e quantificação de proteínas de reserva durante o processo germinativo de sementes de P. pyramidalis, S. brasiliensis and S. obtusifolium. Dois lotes de sementes de S. obtusifolium de vigor diferente foram utilizados. Quatro repetições de 20 sementes de P. pyramidalis, S. brasiliensis e S. obtusifolium, foram colocados em gerbox com papel germitest umedecido com água destilada e incubados durante 72, 200 e 624 horas. Antes e após a embebição as sementes foram pesadas até a extração sequencial e quantificação de proteínas de reserva. A germinação das sementes de P. pyramidalis, S. brasiliensis e S. obtusifolium tem um padrão de embebição trifásico bem definido. Assim, podemos concluir que a germinação das sementes de P. pyramidalis, S. brasiliensis e S. obtusifolium apresenta modelo trifásico na embebição bem definido. O conteúdo de proteínas de reserva de P. pyramidalis e S. brasiliensis degrada na medida em que suas sementes embebem. Os teores de albuminas, globulinas e glutelinas reduzem na medida em que as sementes de S. obtusifolium embebem. Palavras-chave: Floresta tropical seca. Germinação. Poincianella pyramidalis. Mobilização de reservas. Schinopsis brasiliensis. Sideroxylo...

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Section: Resultsmentioning

confidence: 87%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Quantification of storage proteins during seed imbibition of native species from the brazilian caatinga vegetation

Silva

Dantas

2016

SCA

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Seedlings of dry forest-adapted species resume growth after desiccation

da Silva,

de Oliveira,

do Nascimento Araujo

et al. 2024

Braz. J. Bot

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Discontinuous hydration alters seed germination under stress of two populations of cactus that occur in different ecosystems in Northeast Brazil

Lima

Meiado

2017

Seed Sci. Res.

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The water uptake by the seed in arid and semi-arid ecosystems may not be continuous, but may occur in hydration and dehydration cycles (HD). In order to investigate the benefits of discontinuous hydration on seed germination of Pilosocereus catingicola subsp. salvadorensis subjected to environmental stresses, an imbibition curve was determined and times X, Y and Z selected, corresponding to 25, 50 and 75% of the first phase of imbibition, respectively. Seeds of two populations (Caatinga and Restinga) were subjected to 0, 1, 2 and 3 HD cycles, with 48 h of dehydration. Water and saline stress responses were tested at five osmotic potentials (0.0, –0.1, –0.3, –0.6 and –0.9 MPa). Discontinuous hydration provided greater tolerance to water and saline stress in the seeds from the Caatinga region. When the seeds were subjected to water stress, the reduction of the mean germination time (MGT) provided by HD cycles decreased with increasing concentration of the solution. In saline stress, the reduction of MGT was greater in the solutions with the highest concentrations. We conclude that the seeds of the same species collected from populations located in different ecosystems have different germination responses after passage through discontinuous hydration and HD cycles. This provides greater tolerance to environmental stresses but with different responses among populations.

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Sementes de feijão submetidas a ciclos e períodos de hidratação-secagem

Cited by 12 publications

References 13 publications

Quantification of storage proteins during seed imbibition of native species from the brazilian caatinga vegetation

Quantification of storage proteins during seed imbibition of native species from the brazilian caatinga vegetation

Seedlings of dry forest-adapted species resume growth after desiccation

Discontinuous hydration alters seed germination under stress of two populations of cactus that occur in different ecosystems in Northeast Brazil

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