Quantification of storage proteins during seed imbibition of native species from the brazilian caatinga vegetation

Abstract

Poincianella pyramidalis (Fabaceae), Schinopsis brasiliensis (Anacardiaceae) and Sideroxylon obtusifolium (Sapotaceae) are native species of the Caatinga vegetation from northeastern Brazil and have both biological importance and potential economic uses. Little is known about the water uptake and degradation of storage proteins during seed germination of these species. The aim of this study was to evaluate the imbibition and quantify the amount of storage proteins during seed germination of P. pyramidalis, S. brasiliensis and S. obtusifolium. Two lots of S. obtusifolium seeds with different vigour were used. Four replicates of 20 seeds of P. pyramidalis, S. brasiliensis and S. obtusifolium, were sown onto gerboxes with blotting paper soaked in distilled water and incubated during 72, 200 and 624 hours. Before and after imbibition seeds were weighed and frozen at until the sequential extraction and analysis of the seed storage proteins. Based on our results, we conclude that seed germination of P. pyramidalis, S. brasiliensis and S. obtusifolium has a well-defined triphasic imbibition. All storage proteins content of P. pyramidalis and S. brasiliensis seeds degraded along with the seed imbibition. Likewise, the content of albumins, globulins and glutelins decreased as S. obtusifolium seeds absorbed water. Key words: Germination. Poincianella pyramidalis. Reserve mobilisation. Schinopsis brasiliensis. Sideroxylon obtusifolium. Tropical dry forest. ResumoPoincianella pyramidalis (Fabaceae), Schinopsis brasiliensis (Anacardiaceae) e Sideroxylon obtusifolium (Sapotaceae) são espécies nativas da Caatinga e que apresentam grande importância biológica e potencial econômico. Pouco se sabe sobre a absorção de água e degradação de proteínas de reserva durante a germinação dessas espécies. O objetivo deste trabalho foi avaliar a embebição e quantificação de proteínas de reserva durante o processo germinativo de sementes de P. pyramidalis, S. brasiliensis and S. obtusifolium. Dois lotes de sementes de S. obtusifolium de vigor diferente foram utilizados. Quatro repetições de 20 sementes de P. pyramidalis, S. brasiliensis e S. obtusifolium, foram colocados em gerbox com papel germitest umedecido com água destilada e incubados durante 72, 200 e 624 horas. Antes e após a embebição as sementes foram pesadas até a extração sequencial e quantificação de proteínas de reserva. A germinação das sementes de P. pyramidalis, S. brasiliensis e S. obtusifolium tem um padrão de embebição trifásico bem definido. Assim, podemos concluir que a germinação das sementes de P. pyramidalis, S. brasiliensis e S. obtusifolium apresenta modelo trifásico na embebição bem definido. O conteúdo de proteínas de reserva de P. pyramidalis e S. brasiliensis degrada na medida em que suas sementes embebem. Os teores de albuminas, globulinas e glutelinas reduzem na medida em que as sementes de S. obtusifolium embebem. Palavras-chave: Floresta tropical seca. Germinação. Poincianella pyramidalis. Mobilização de reservas. Schinopsis brasiliensis. Sideroxylo...

Assessment of Genetic Diversity in Bambara Groundnut (Vigna subterranea) Landraces Based on Seed Storage Proteins Electrophoretic Pattern

“…Storage protein fractions were sequentially extracted based on their solubility (Silva et al, 2016). Seeds flour (1g) was dissolved in 10 mL distilled water, the homogenate was incubated for 30 minutes at 30 °C and subsequently centrifuged at 3000 x g for 20 minutes.…”

Seed storage protein changes and mobilization pattern in Bambaranut (Vigna subterranea) (L.) Verdc. during germination

“…Storage protein fractions were sequential extracted based on their solubility (22). Seeds flour (1 gm) was dissolved in 10 ml distilled water, the homogenate incubated for 30 min at 30 °C and subsequently centrifuged at 3000 x g for 20 min.…”