In vitro conservation of diploid banana accessions

Abstract

A protocol for the in vitro conservation of diploid banana accessions based on lower temperature for culture environment was developed. Twenty four genotypes, four clones per genotype were studied. After disinfection, shoot tips (0.5 cm diameter x 0.5 cm height) were established in vitro and submitted to multiplication induced by benzylaminopurine. Twelve explants (0.6 cm diameter x 1.2 cm height) from each accession were transferred to the MS medium supplemented with 20 g L-1 sucrose and 2 g L-1 Phytagel, without growth regulators, under light intensity of 2000 lux, 16-hour photoperiod, at temperatures of: 17 ± 2ºC, 22 ± 2ºC and 26 ± 2ºC. The development of plantlets was evaluated during 450 days and the survived plantlets were acclimated under greenhouse conditions. There were no differences among genotypes concerning the behavior during in vitro conservation; all genotypes showed potential to be conserved for a long time. The interval for transference should be 180, 360 and 450 days under the mean temperatures of 26, 22 and 17ºC, respectively.

<b>Univariate and multivariate statistical tools for <i>in vitro</i> conservation of citrus genotypes

“…These type of responses have been observed in pineapple (Canto et al, 2004;Souza et al, 2006), cassava (Souza et al, 2009), sugarcane (Lemos, Ferreira, Alencar, Albuquerque, & Ramalho Neto, 2002), grapevine (Silva, Luis, & ScherwinskiPereira, 2012), banana (Oliveira, Silva, Silva, & Silveira, 2000) and potato (Conceição, Fortes, & Silva, 1999).…”

“…In sugarcane, Lemos et al (2002) reported that it is possible to conserve microplants under slow-growth conditions for 12 months when they are maintained at a temperature of 15°C light intensity of 50 μmol m -2 s -1 and a 16 hours photoperiod. Oliveira et al (2000) found that the interval between the transfer of diploid banana accessions conserved in vitro should be 180, 360 and 450 days, when they are maintained at a mean temperature of 26, 22 and 17°C, respectively, 2000 and performed only one or two subcultures per year. They concluded that the protocol could easily be applied to many species and parental cultivars of citrus while waiting for the development of better techniques.…”

SHORT TERM PRESERVATION FOR FIG (Ficus carica cv. Black fig) BY DIFFERENT OSMOTIC STABILIZERS

“…In vitro preservation of vegetative propagated genetic resources provided an effective conservation system for the guarantee of food supplies. De Oliveira et al (2000) stated that in vitro conservation by minimal growth rate of the plantlets can be achieved through adding osmotic stabilizers such as sucrose, sorbitol, or mannitol in culture medium and/or by decreasing the temperature in the conservation chambers .They also reported that several researchers recommend the use of temperatures between 15C and 21C in conservation chambers in order to decrease the growth rate. In the present study, after three months of conservation, sorbitol was perceived as the suitable osmotic agent for fig (Ficus carica) shortterm conservation at a concentration of 50 g/L in 5C, where our results were supported by the findings of Brundus and Constantirocicia (2012) who conducted a similar study on potato "Solanum tuberosum" explant conservation for 17 months, they found that sorbitol inhibited the growth of plantlets and no significant difference occurred in their traits.…”

Genetic Improvement of Banana Using Conventional andIn VitroTechnologies

“…In vitro (tissue culture-based) conservation has been established and practiced worldwide to complement field and seed-bank conservation (Oliveira et al 2000). Presently, culture-based technologies are the most suitable and preferred approaches to conserve and distribute banana germplasm.…”