Molecular characterization of Listeria monocytogenes isolated from animal products in a city of Northern Brazil

El objetivo de esta investigación fue determinar la prevalencia de la bacteria Listeria monocytogenes. Para ello, se analizaron 60 muestras de salchicha tipo Huacho de cinco mercados de abasto del Cercado de Lima, que se procesaron en el Laboratorio de Microbiología de la Facultad de Farmacia y Bioquímica de la UNMSM, dentro de las 24 horas de su recolección. Se utilizó la metodología USDA-FSIS, en sus tres fases: pre-enriquecimiento, enriquecimiento de la muestra y siembra en agares selectivos y prueba de CAMP. Los resultados mostraron que la prevalencia de esta bacteria en las salchichas tipo Huacho es de 78%. Se concluye que este producto podría constituir un alimento de riesgo potencial para la salud pública.

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“…En Brasil, Monteiro y col, analizaron 30 muestras de salichicha, aislando, en el 6,7% de ellas, L. monocytogenes (serotipos 1/2a y 1/2b) (16) .…”

Section: Referencias Bibliográficasunclassified

Prevalencia de Listeria monocytogenes en salchipapas tipo Huacho provenientes de los mercados de abasto del Cercado de Lima

Pérez¹,

Salazar

Gamarra

2013

Ciencia e investigación

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“…Moreover, in 2010, 1,601 confirmed cases of listeriosis were reported in Europe, 17 % of which were fatal, with the highest rates of infection observed in Finland, Denmark and Spain (EFSA, 2012). In Brazil, listeriosis outbreaks are not well documented and information is lacking regarding foodborne illnesses (MONTEIRO et al, 2013). However, in Brazil, a few studies have indicated the presence of L. monocytogenes in foods, including meat products (ROSSI et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Packaging properties and control of Listeria monocytogenes in bologna by cellulosic films incorporated with pediocin

Espitia

Pacheco

Melo

et al. 2013

Braz. J. Food Technol.

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Listeria monocytogenes is a foodborne pathogen, able to survive and proliferate at refrigeration temperatures. As a result, ready-to-eat meat products have been associated with major outbreaks. Producing meat products involves lethal preservation treatments, e.g. thermal treatments. Listeria contamination, however, may be introduced when products are sliced and packaged at retail businesses or delicatessens. In Brazil, sliced bologna is very popular at retail markets. After slicing, however, bologna has a short shelf-life. The aim of this work was to study the effects of pediocin incorporation on the load at break, water vapor permeability rate and structure, by microscopic analysis, of antimicrobial cellulosic packaging. The potential application of the developed packaging for the preservation of bologna and inhibition of Listeria biofilm formation was also studied. Cellulosic antimicrobial packaging films were produced with cellulose acetate and acetone. Pediocin (commercially available concentrate ALTA TM 2341) was incorporated at 30, 40 and 50 % w/w. The load at break of films was studied using the Universal Testing Machine (Instron) at 10 °C and 25 °C. The water vapor permeability was determined by gravimetric method. A scanning electron microscope was used to study the developed packaging structure. Antimicrobial activity of films against Listeria innoucua and L. monocytogenes was tested both in vitro and in bologna samples. Results showed that values of load at break decreased with increasing concentrations of pediocin at 10 °C and 25 °C. Regarding water vapor permeability, only the control and 50 % pediocin films presented statistical difference, with the 50 % pediocin film being more permeable. In vitro tests showed antimicrobial activity against L. innocua. Cellulosic film with 50 % pediocin reduced L. monocytogenes growth on sliced bologna by 1.2 log cycles after 9 days and prevented biofilm formation on packaging and bologna surfaces. Hence, active cellulosic films made with 50 % pediocin in the form of commercially available concentrated ALTA™ 2341 have the potential of being used in a system of hurdle technologies as a final obstacle for L. monocytogenes control in bologna preservation.

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Molecular Methods for the Detection and Characterization of Food‐Borne Pathogens

Rusul¹,

Chuah²

2015

Advances in Food Biotechnology

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Molecular characterization of Listeria monocytogenes isolated from animal products in a city of Northern Brazil

Abstract: Listeria monocytogenes, a foodborne pathogen causes listeriosis, a fatal disease in about 30% of cases that affects mainly immunocompromised persons. The aim of this research was to characterize L. monocytogenes pulsed-fi eld gel electrophoresis

Cited by 8 publications

References 21 publications

Prevalencia de Listeria monocytogenes en salchipapas tipo Huacho provenientes de los mercados de abasto del Cercado de Lima

Prevalencia de Listeria monocytogenes en salchipapas tipo Huacho provenientes de los mercados de abasto del Cercado de Lima

Packaging properties and control of Listeria monocytogenes in bologna by cellulosic films incorporated with pediocin

Molecular Methods for the Detection and Characterization of Food‐Borne Pathogens

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