Crioprotetores etileno glicol ou acetamida na viabilidade in vitro de espermatozoides congelados de ovinos

Silva, Ellen Cordeiro Bento da; Cajueiro, Jobson Filipe de Paula; Silva, Sildivane Valcácia; Guerra, Maria Madalena Pessoa

doi:10.1590/s0103-84782012005000027

Cited by 3 publications

(1 citation statement)

References 18 publications

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“…Para esses autores, os tratamentos com a amida foram incapazes de preservar a motilidade progressiva e as estruturas dos espermatozoides congelados, tornando as amostras inviáveis para inseminação artificial. Em outro trabalho utilizando acetamida (2% e 7%), Silva et al (32) encontraram médias significativamente menores de MP, VIG e integridade estrutural da membrana plasmática em relação ao diluidor com glicerol (GL5%). Segundo os autores, o dano osmótico causado pela acetamida possivelmente foi a principal causa dos baixos índices de congelabilidade dos espermatozoides ovinos.…”

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Dimetilacetamida Associada Ou Não Ao Glicerol Para Criopreservação De Sêmen Ovino

et al. 2018

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Resumo O objetivo do presente estudo foi testar a dimetilacetamida (DMA) em diferentes concentrações, associada ou não ao glicerol (GL), sobre a viabilidade espermática do sêmen ovino congelado. Foram utilizados 10 ejaculados de dois carneiros adultos da raça Santa Inês. Os ejaculados foram divididos em sete grupos experimentais, respeitando o limite máximo de 5% de DMA, sendo eles: GL6%, DMA3%, GL5%+DMA1%, GL4%+DMA2%, GL3%+DMA3%, GL2%+DMA4%, GL1%+DMA5%. Os espermatozoides criopreservados nos diferentes tratamentos foram analisados quanto à cinética subjetiva, integridade estrutural da membrana plasmática (EOS), integridade funcional da membrana plasmática (CO) e morfologia espermática, observando defeitos totais (DT) e defeitos maiores (DM). A motilidade total (MT) e a progressiva (MP) pós-descongelação nos grupos GL5%+DMA1%; GL4%+DMA2% e GL3%+DMA3%, foram semelhantes (P>0,05) ao tratamento controle (GL6%). Destes, o diluidor GL4%+DMA2% foi o único que promoveu a manutenção da MT e MP pós-descongelação, quando comparado com o sêmen in natura (P>0,05). Não foram observadas diferenças significativas (P>0,05) para os parâmetros de EOS, CO, DT e DM nos diferentes grupos avaliados. A dimetilacetamida associada ao glicerol mostrou-se eficaz na manutenção da viabilidade espermática em ovinos, avaliada pós-descongelação. Entretanto, foi observado efeito deletério da DMA nas concentrações mais elevadas ou quando não esteve associada ao glicerol.

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Section: Discussionunclassified

Dimetilacetamida Associada Ou Não Ao Glicerol Para Criopreservação De Sêmen Ovino

et al. 2018

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Dimethylformamide Preserves the Integrity of Cryopreserved Goat Semen in a Soybean Lecithin-Based Extender

Silva

Monteiro

Costa

et al. 2022

Biopreservation and Biobanking

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ADDITIVES USED IN EXTENDERS TO IMPROVE THE FREEZABILITY OF RAM SEMEN IN RECENT YEARS: a mini review

Öztürkler¹,

Ari²

2017

S-h. biol.

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A b s t r a c tOne of the most important reasons why artificial insemination does not spread as much in cattle as sheep is that ram sperm is highly fragile against cryodamage and consequently the optimum fertility results can not be obtained from cervical inseminations compared to laparoscopic insemination. To establish an ideal ram sperm freezing model, many studies have been carried out for more than 60 years and various methods have been tested by making great efforts. One of the topics where these tests have been done intensively in recent years are additives used in freezing extenders in order to increase the tolerance of sperm against oxidative stress in frozen-thawed ram semen. In this review, covering the studies carried out between the years 2000-2016, we have mostly compiled the additives used in ram semen freezing media which have given better results compared to others supplements. It is understood that, usually, seminal plasma and proteins, thiol compounds, enzymatic antioxidants, sugars, fatty acids and vitamins have been studied as additive agents in recent years. As a result, this review suggests us that further studies are needed to explore the novel techniques and new additives, and their combinations should be tested in different doses in order to install an ideal cryopreservation template for ram semen. Furthermore, even though addition of various compounds to freezing media have improved freezability of ram semen in experimental conditions, anyway these results must be confirmed in field studies. Therefore, the value of sperm which potential fertility is predicted from laboratory survey must be compared with conception or lambing rates.Keywords: ram semen, additives, freezability, extender While the basic techniques related to sperm freezing have done positive effects and important advances mostly on dairy cattle husbandry, any remarkable success has not been provided in sheep. Substantially, these matter is caused mainly by from two reasons following as: i] intolerance of ram sperm against cryopreservation, ii] the physiological and anatomic factors in sperm transport. It is well known subject for a long time that freezing and thawing of ram sperm have caused significant membran and genetic damage [1]. As cervical insemination has given poor fertility results compared to laparascopic inseminations, artificial insemination with frozen semen has not become widespread in sheep husbandry [2]. On the other hand, the expenses of ram semen freezing are almost 20 times more than those of bull semen freezing cost [3]. В Recently, investigators have focused mostly on especially composition, determination of in vivo properties and effects of different cryoprotectants, antioxidants and specific supplements in sperm freezing [4].We understood from literature documented here, no comprehensive review of the researches on frozen ram sperm has been found from that date into the past 16 years. During 2000s, the attentions of researchers have mostly concentrated on additives for improving membrane repair and ...

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Crioprotetores etileno glicol ou acetamida na viabilidade in vitro de espermatozoides congelados de ovinos

Abstract: IVCrioprotetores etileno glicol ou acetamida na viabilidade in vitro de espermatozoides congelados de ovinos

Cited by 3 publications

References 18 publications

Dimetilacetamida Associada Ou Não Ao Glicerol Para Criopreservação De Sêmen Ovino

Dimetilacetamida Associada Ou Não Ao Glicerol Para Criopreservação De Sêmen Ovino

Dimethylformamide Preserves the Integrity of Cryopreserved Goat Semen in a Soybean Lecithin-Based Extender

ADDITIVES USED IN EXTENDERS TO IMPROVE THE FREEZABILITY OF RAM SEMEN IN RECENT YEARS: a mini review

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