Criptococose pulmonar associada à infecção sistêmica por Corynebacterium pseudotuberculosis em cabra (Capra hircus)

Oliveira, Laura Iglesias de; Prado, Juliana da Silva; Cunha, Bernardo Melo da; França, Ticiana N.; Rocha, Luiz Fabio de Oliveira da; Carrocino, Renata Lins; Brito, Marilene de Farias

doi:10.1590/s0103-84782011005000084

Cited by 2 publications

(3 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Corrobora essa hipótese o fato de que, no presente caso, o ovino apresentava endoparasitismo por nematódeos (Trichostrongyloidea) e, apesar da coprocultura não ter sido realizada, o parasitismo concomitante por Haemonchus contortus era esperado, visto a alta prevalência de hemoncose em rebanhos ovinos do semiárido [21] e a comum ocorrência da doença na propriedade, somado à perda de peso e a anemia identificada no hemograma do ovino, achados compatíveis com quadro de haemoncose. Convém ressaltar que já foram descritos casos de criptococose em caprinos portadores de Mycobacterium bovis e Corynebacterium pseudotuberculosis [11,17,23].…”

Section: Discussionunclassified

“…No presente estudo, o quadro clínico e os achados anatomopatológicos foram semelhantes aos descritos em um caso envolvendo um ovino diagnosticado com criptococose nasal em Pernambuco [36]. Cabe ressaltar que, em ambos os ovinos, não foram observadas lesões pulmonares decorrentes da infecção por Cryptococcus spp., as quais tem sido frequentes em caprinos [14,23,35,40] e, até o momento, relatada somente em um ovino [15].…”

Section: Discussionunclassified

“…No Brasil, a criptococose tem sido descrita nos estados de Mato Grosso [14], Minas Gerais [19], Paraíba [30], Pernambuco [36], Rio de Janeiro [15,23] e São Paulo [17,35], com apenas 2 descrições referindo-se a ovinos [15,36]. Casos de criptococose por C. gattii [1,3,18,35,40] foram descritos apenas em caprinos na Espanha, Itália e Brasil.…”

Section: Introductionunclassified

See 2 more Smart Citations

Nasal Cryptococcosis Caused by Cryptococcus gattii in Sheep in Bahia, Northeastern Brazil

Macêdo

Peixoto

Carvalho

et al. 2020

Acta Scientiae. Vet.

View full text Add to dashboard Cite

Background: Cryptococcosis is a potentially fatal systemic mycosis that can affect several animal species. The disease is caused by the yeast species Cryptococcus neoformans and C. gattii and is usually associated with immunosuppressive diseases or debilitating factors. In Brazil, cryptococcosis in ruminants has been described in several states, but there have been only two instances of infection in sheep. Until the present, cryptococcosis caused by C. gattii has only been reported in goats. The objective of this study was to describe, for the first time, a case of nasal cryptococcosis caused by C. gattiiin a sheep in the state of Bahia.Case: The disease affected a 4-year-old Santa Inês sheep, created in extensive system in the municipality of Ribeira do Pombal, Bahia, Brazil. The animal presented weight loss, with an asymmetric volume increase in the right nasal cavity, accompanied by dyspnea and bloody nasal discharge. Samples of the tumor mass were collected for cytological analysis of yeast structures compatible with Cryptococcus spp. After cytological diagnosis, the sheep was sent to the Livestock Development Center of the Federal University of Bahia. On physical examination, there was a large and firm irregular mass, strongly adhering to the right nasal cavity and causing facial asymmetry. Surgical removal was attempted, but a marked infiltrative character of the lesion was observed, with extensive tissue destruction. Owing to the unfavorable prognosis, euthanasia was carried out followed by necropsy, in which a large irregular mass was observed that almost completely obliterated the right nasal cavity. Resorption of the right nasal bone and the dorsal and middle nasal turbinates was also observed. Fragments of the nasal lesion were collected, fixed in 10% formalin, routinely processed for histopathology, and stained with hematoxylin and eosin (HE) and Mayer's mucicarmine. Samples of this lesion were sent for molecular characterization of the etiologic agent by polymerase chain reaction (PCR). The histopathological evaluation showed hundreds of generally colorless rounded yeasts cells composed of a clear halo, surrounding a slightly basophilic structure, giving the tissue a vacuolated aspect, known as a “soap bubble lesion” There was also intense inflammatory infiltration and extensive areas of necrosis and hemorrhage, which allowed the morphological diagnosis of diffuse granulomatous rhinitis marked with intralesional yeasts compatible with Cryptococcus spp. Additionally, PCR was positive for C. gattii.Discussion: A case of nasal cryptococcosis caused by C. gattii in sheep in the state of Bahia is described for the first time, diagnosed using clinical-epidemiological, cytological, anatomopathological, and molecular data. Airborne infection occurs by inhaling encapsulated spores present in organic matter, such as decomposing vegetables and bird and bat excreta as observed on the farm where the outbreak occurred. It is important to highlight that sheep and goats affected by cryptococcosis can contaminate the environment, and it is prudent to consider animals with the disease as a risk to public health and eliminate them from the herd, given the costs of the few therapeutic options available on the market. It should be noted that in cases of tumor lesions, which are common in cryptococcosis, cytological examination should be recommended, mainly for screening and distinguishing neoplastic and inflammatory lesions. However, anatomopathological and molecular evaluations are essential for the differential diagnosis of other mycotic rhinitis, in particular, conidiobolomycosis and nasal pythiosis.

show abstract

Section: Discussionunclassified

See 1 more Smart Citation

Nasal Cryptococcosis Caused by Cryptococcus gattii in Sheep in Bahia, Northeastern Brazil

Macêdo

Peixoto

Carvalho

et al. 2020

Acta Scientiae. Vet.

View full text Add to dashboard Cite

show abstract

Nitric oxide and immune response in infection control of Caseous Lymphadenitis

Neto

Santos

Fraga

et al. 2017

Arq. Bras. Med. Vet. Zootec.

View full text Add to dashboard Cite

This study aimed to evaluate aspects of host immune response using an experimental infection model of Corynebacterium pseudotuberculosis (CP) in C57/Black6 wild-type and knockout for nitric oxide (KO-NO) mice. 28 mice were evaluated: 4 wild-type controls; 10 wild-type infected with CP; 4 KO-NO controls; 10 KO-NO infected with CP. Infection procedures were carried out by intraperitoneal inoculation using 10 7 . Infected C57/Black6 KO-NO mice began to die after the 5° day post-inoculation, up until the 14º day. Neutrophils were found in increased numbers in the infiltrate of KO-NO murine peritoneal cavities. Examination of splenic tissue revealed an accumulation of lymphocytes, predominantly CD8 T-cells, in experimental animal groups. KO-NO animals were found to have a predominance of granulomas 7 days post-inoculation, primarily in the lymph nodes. In addition, greater amounts of bacteria were recovered from the mesenteric lymph nodes of KO-NO mice. There was no statistically significant difference in the levels of total IgG and its subclasses 14 days post-inoculation between KO-NO and wild groups. The results suggest the importance of nitric oxide in the process of controlling CP infection, as KO-NO animals were observed to be markedly more affected by infection with this bacterium.Keywords: nitric oxid, knockout mice, Corynebacterium pseudotuberculosis, caseous limphadenitis RESUMO O objetivo deste estudo foi avaliar os aspectos da resposta imune do hospedeiro, mediante o uso de um modelo experimental de infecção de Corynebacterium pseudotuberculosis (CP) em camundongos C57/Black6 do tipo selvagem e em C57/Black6 knockout para o óxido nítrico (KO-NO

show abstract

Criptococose pulmonar associada à infecção sistêmica por Corynebacterium pseudotuberculosis em cabra (Capra hircus)

Abstract: IVCriptococose pulmonar associada à infecção sistêmica por Corynebacterium pseudotuberculosis em cabra (Capra hircus)

Cited by 2 publications

References 11 publications

Nasal Cryptococcosis Caused by Cryptococcus gattii in Sheep in Bahia, Northeastern Brazil

Nasal Cryptococcosis Caused by Cryptococcus gattii in Sheep in Bahia, Northeastern Brazil

Nitric oxide and immune response in infection control of Caseous Lymphadenitis

Contact Info

Product

Resources

About