Heparina e Na2EDTA como anticoagulantes para surubim híbrido (Pseudoplatystoma reticulatum x P. corruscans): eficácia e alterações hematológicas

Abstract

EDTA como anticoagulantes para surubim híbrido (Pseudoplatystoma reticulatum x P. corruscans): eficácia e alterações hematológicas Heparin and Na 2 EDTA as anticoagulants for hybrid surubim catfish (Pseudoplatystoma reticulatum x P. corruscans): efficacy and hematological changes I Pesquisadores da Embrapa Agropecuária Oeste, BR 163, km 253,6, CP 661,

Na2EDTA anticoagulant impaired blood samples from the teleost Piaractus mesopotamicus

“…Walencik & Witeska (2007) observed higher fragility in common carp erythrocytes to hipotonic solution since the lesser concentration of Na 2 EDTA tests (0.01mg ml -1 ) and a positive relation of the osmotic fragility and the increase of the anticoagulant concentrations. Similar results were described in Blennius pholis (Mainwaring & Rowley 1985), Oncorhynchus mykiss (Korcock et al 1988), Oreochromis niloticus (Ekanem et al 2012), Pseudoplatystoma reticulatum x P. corruscans (Ishikawa et al 2010) and Colossoma macropomum (Pádua et al 2012).…”

“…Ten fish were captured using a net and mechanically immobilized in a wet piece of fabric. The sampling of the blood of each fish was realized through veno-caudal puncture, using sterile syringes (3mL) and hypodermic needles (25x7mm), containing no anticoagulants, as recommended by Ishikawa et al (2010). Trying to not promote any hemolitic effects besides the possibly caused by the anticoagulants, we decided to use no anesthetic chemicals (Korcock et al 1988).…”

“…The blood collected, circa of 0.5mL, was quickly placed in four polyethylene tubes (1.5mL): The first and second contained 15μL of NA 2 EDTA, at a concentration of 3% (0.3mg mL -1 of blood) and 10% (1mg mL -1 of blood), respectively. At last, the third and fourth tubes contained 15 μL of sodium heparin at concentration of 5.000 UI (75 UI mL -1 of blood) and 100 UI (1.5 UI mL -1 of blood), obtained from the dilution of the heparin 5.000 UI in physiological solution (0.65% of NaCl, 1:50) (Ishikawa et al 2010).…”

“…On the other hand, the EDTA is able to chelate ions, including calcium (Ca2+), important in several steps of the blood clotting (Harr et al 2005). However, according to Ishikawa et al (2010), both anticoagulants can promote changes on the blood parameters in teleost fish.…”

Comparação de metodologias utilizadas na análise dos parâmetros sanguíneos e da proteína total de tilápia do Nilo (Oreochromis niloticus)

“…Dentre as funções desses compostos está a conservação da fluidez sanguínea e manutenção das características celulares. A comparação entre eles tem sido alvo de recentes estudos (ISHIKAWA et al, 2010;WITESKA & WARGOCKA, 2011). Alguns autores consideram a heparina como o anticoagulante mais adequado para as análises hematológicas de peixes (HATTINGH & SMITH, 1976;MAINWARING & ROWLEY, 1985).…”