Heparin-binding proteins of seminal plasma in Nellore bulls

Fernandes, Carlos Eurico dos Santos; Souza, Fabiana Ferreira de; Souza-Neto, Jayme A.; Ribola, Paulo Eduardo Martins

doi:10.1590/s0103-84782008005000044

Cited by 5 publications

(5 citation statements)

References 9 publications

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“…Further, the concentration of a 45 kDa protein was significantly (P<0.05) higher in bulls with average fertility than in their good and poor counterparts. Similar studies in seminal plasma of Nellore bulls (Fernandes et al 2009), rams (Martins et al 2013) and human (Kumar et al 2009) identified 8, 13 and 17 bands of affinity purified HBP with molecular weights ranging from 15 to 63 kDa, 10 to 232 kDa and 10 to 90 kDa, respectively. Further, Arangasamy et al (2005) and Kumar et al (2008) observed eight (11, 14, 18, 20, 31, 41, 65 and 71 kDa) and six (14, 20, 24, 31, 41 and 65 kDa) major HBP on SDS-PAGE in buffalo seminal plasma, respectively.…”

Section: Resultssupporting

confidence: 56%

Prediction of buffalo bull fertility based on sperm motion traits, function tests and expression of heparin binding protein

Singh

Brar

Cheema

et al. 2017

Indian J of Anim Sci

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Selection of good fertility bulls is a challenge since large variations exist in the result of tests applied for semen evaluation. Therefore, the present study was undertaken to predict the fertility of buffalo bulls on the basis of sperm motion traits, function tests and expression of heparin binding protein (HBP). Both fresh and frozen semen from breeding Murrah buffalo bulls (30) were procured from 2 government bull farms. The frozen-thawed semen was used to evaluate first service conception rate (FSCR), CASA-based motility (total and progressive) and function tests (HOST, acrosome reaction and DNA integrity). On the basis of FSCR, all bulls were divided into 3 groups, viz. good fertility bulls (GFB, >50%), average fertility bulls (AFB, 30-49.9%) and poor fertility bulls (PFB, <30%). Additionally, fresh and frozen-thawed semen were analyzed for quantification and characterization of HBP through affinity chromatography. CASA based total motility and acrosome reaction were significantly higher in GFB as compared to their counterparts. No significant difference between good and average fertility bulls was observed for percent HOST and progressive motility. The HBP was 6.6±0.2 mg/ml, 0.63±0.02 mg/109 sperms and 0.36±0.02 mg/109 sperms in seminal plasma, fresh- and frozen-thawed spermatozoa, respectively. Although, there was no significant difference in the quantity of total HBP in semen with respect to bull fertility, a specific trend definitely existed. The HBP peak was separated into proteins of 135, 100, 75, 65, 48, 45, 40, 37, 31, 28, 26, 20, 18, 16 and 11 kDa in seminal fluid. Overall, the purified HBP indicated the presence of 65, 31, 20 and 11 kDa proteins in seminal plasma, fresh- and frozen-thawed sperm extracts of good fertility bulls and seemed to be candidate proteins to predict buffalo bull fertility. It was concluded that CASA-based total motility, acrosome reaction and purified HBP-65, HBP-31, HBP-20 and HBP-11 in buffalo bull sperm could be used for differentiation of good fertility bulls from poor fertility ones.

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Section: Resultssupporting

confidence: 56%

Prediction of buffalo bull fertility based on sperm motion traits, function tests and expression of heparin binding protein

Singh

Brar

Cheema

et al. 2017

Indian J of Anim Sci

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“…Another group of proteins investigated in the present study were heparin binding proteins. Until now, profiles of HBPs derived from seminal plasma and sperm membranes have been associated with bull fertility (Fernandes et al 2009). The majority of HBPs found in the YF of red deer were low-molecular weight proteins.…”

Section: Discussionmentioning

confidence: 99%

Polish Journal of Veterinary Sciences

Orzołek

Dyrda

Rafalska

et al. 2021

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“…But, in the present study, proteins of 13, 17, 20, 25 showed an affinity for heparin. Electrophoretic analysis of affinity purified HBP fraction from SP of Nellore bulls (Fernandes et al, 2009), rams (Martin et al, 2013), buffalo bulls (Singh et al, 2014) and cross bred bulls (Cheema et al, 2016) revealed the presence of 8 (15 -63 kDa), 13 (10 -232 kDa), 8/6/9 (13 -71 kDa/14 -61 kDa/10 -170 kDa) and 14 (14-150 kDa) bands, respectively. Therefore, variation in number of HBP bands was also evident in other species.…”

Section: Characterization Of Seminal Plasma and Sperm Proteins By Sdsmentioning

confidence: 99%

Proteins and Heparin Binding Proteins of Spermatozoa and Seminal Plasma in Beetal Bucks: Purification, Characterization and Role in In vivo Fertility

Dhillon

Cheema

Singhal

2020

IJAR

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Background: The proteome analysis of seminal plasma and spermatozoa is of special relevance in livestock. Heparin binding proteins (HBPs) found in the seminal plasma of several mammals are shown to bind to sperm membrane and affect a series of events that contribute to normal fertility, such as sperm capacitation, formation of the oviduct reservoir and binding to the oocyte. Profiles of HBPs from seminal plasma and sperm membranes have been associated with sperm fertility. Although, HBPs present in the SP are described in several species, but little is known about HBPs in buck. Methods: Seminal plasma (SP) and sperm extracts (SE) of 13 bucks were subjected to heparin-sepharose affinity chromatography. Sperm extract, seminal plasma and purified HBPs and Non-HBPs were fractionated by SDS-PAGE. Total 78 females (6 per buck) were mated with 13 bucks. Bucks were divided into two groups, G-I (high fertile, 83.3-100% FR) and G-II (low fertile, 50-66.7% FR). Relationship between HBPs and fertility rate was observed. Result: SDS – PAGE of SP and SE resulted in resolution of 22 (10-240 kDa) and 21 (10-270 kDa) bands, respectively. Based on fertility rate 15 and 13 kDa proteins were absent in SP of higher number of GI-compared to G-II bucks. Fourteen bands ranging from 10 – 180 kDa and 10 – 150 kDa were separated from SP-NHBP and SP-HBP. SP-HBPs of 75, 35, 30, 28, 25 and 13 kDa were present in higher (28.6%, 42.5%, 26.2%, 40.5%, 14.3% and 36.2%) number of high fertile than low fertile bucks. NHBP and HBP purified from SE resolved into 11 bands ranging from 10 – 135 kDa and 10 – 120 kDa, respectively. SE-HBP of 53 kDa, 50/45 kDa and 25 kDa were present in higher percentage of high fertile than low fertile bucks.

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Heparin-binding proteins of seminal plasma in Nellore bulls

Cited by 5 publications

References 9 publications

Prediction of buffalo bull fertility based on sperm motion traits, function tests and expression of heparin binding protein

Prediction of buffalo bull fertility based on sperm motion traits, function tests and expression of heparin binding protein

Polish Journal of Veterinary Sciences

Proteins and Heparin Binding Proteins of Spermatozoa and Seminal Plasma in Beetal Bucks: Purification, Characterization and Role in In vivo Fertility

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