Resposta imune-humoral e celular em bovinos da raça Nelore imunizados com extrato de larvas (L2 e L3) de Dermatobia hominis (Linnaeus Jr., 1781)

Fernandes, Nelson Luis Mello; Socol, Vanete Thomaz; Pinto, Simone Benghi; Minozzo, João Carlos; Oliveira, Carlos Antônio Lopes de

doi:10.1590/s0103-84782007000300029

Cited by 3 publications

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References 5 publications

(4 reference statements)

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“…However, cytological tissue changes (or damage) in warble-infested skin has been poorly studied under natural or controlled conditions. Circulating leucocytes in hosts with D. hominis myiasis are reported in cattle 1,8 and rats 10 . Except for observations of alterations in response to migration of the L 1 of Cuterebra and Hypoderma, there have been few studies of the host cellular pathways in skin myiasis 6,26 .…”

Section: Discussionmentioning

confidence: 99%

Spleen cell proliferation during and after skin myiasis by human bot fly Dermatobia hominis

Gonçalves¹,

Nascimento²,

Breyner³

et al. 2009

Rev. Inst. Med. trop. S. Paulo

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SUMMARYSpleen cells from mice were examined at 5, 10, 15, 20 and 25 days post-infection (dpi) with Dermatobia hominis larva and at 5, 10, 15, 30 and 60 days post-larval emergence (dple). Cell proliferation in vitro assays were carried out with RPMI-1640 medium and larval secretory product (LSP) of D. hominis at 5, 10, 15, 20 and 25 days. When each group of mice was tested against each medium, significance was only seen for 25 dpi, with increasing order: LSP-10 d, -25 d, -5 d, -20 d, -15 d and RPMI. Significant results were also observed when each medium was tested against mice at each dpi or dple. Each dple group vs. each medium produced significant results only for 10 dple, with increasing order: LSP-5 d, -20 d, -25 d, -10 d, -15 d and RPMI. Comparative tests were also carried out between groups to refine certain observations. The LSPs were also analyzed using SDS-PAGE. The results prove that myiasis caused depletion of spleen cells, particularly under the effect of the LSP-10 and -15, but the cells tended to increase up to 60 dple. This in vitro assay may represent the real systemic immune response in the relationship LSP-D. hominis-host.

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Section: Discussionmentioning

confidence: 99%

Spleen cell proliferation during and after skin myiasis by human bot fly Dermatobia hominis

Gonçalves¹,

Nascimento²,

Breyner³

et al. 2009

Rev. Inst. Med. trop. S. Paulo

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“…The negative control of the reaction was performed using the serum collected from the same rabbit on day 0 of the experiment, prior to inoculation with the antigenic extract. The serum collected on day 45 of the experiment was used as a positive control, in accordance with the proposal of Fernandes et al 10 The absorbance readings of the ELISA test were performed in the microplate reader (BIORAD Model 550) using a 492nm wavelength filter.…”

Section: Titering Sera Samplesmentioning

confidence: 99%

Production of anti-Cryptosporidium polyclonal antibodies and standardization of direct immunofluorescence for detecting oocysts in water

Osaki

Costa

Troiano

et al. 2011

Rev. Soc. Bras. Med. Trop.

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INTRODUCTION: The production of anti-Cryptosporidium polyclonal antibodies and its use in direct immunofluorescence assays to determine the presence of Cryptosporidium in water are described in the present work. METHODS: Two rabbits were immunized with soluble and particulate antigens from purified Cryptosporidium oocysts. The sera produced were prepared for immunoglobulin G extraction, which were then purified and conjugated with fluorescein isothiocyanate (FITC). Slides containing known amounts of oocysts were prepared to determine the sensitivity of the technique. To test the specificity, slides containing Giardia duodenalis cysts were prepared. RESULTS: The conjugate was successfully used in water samples experimentally contaminated with Cryptosporidium oocysts, and it was possible to detect up to five oocysts/spot, corresponding to contamination of 250 oocysts/mL. CONCLUSIONS: The three immunizations performed in the rabbits were enough to produce antibodies against Cryptosporidium, the standard direct immunofluorescence assay permitted the detection of five oocysts in 20% of the samples, and no cross-reaction with Giardia duodenalis cysts occurred.

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High morbidity cutaneous enzootic myiasis by Dermatobia hominis (Diptera: Oestridae) in sambar deer (Rusa unicolor)

et al. 2020

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Resposta imune-humoral e celular em bovinos da raça Nelore imunizados com extrato de larvas (L2 e L3) de Dermatobia hominis (Linnaeus Jr., 1781)

Abstract: Immune humoral and cellular response of nelore bovines immunized with larvae extract (L 2 and L 3 )of Dermatobia hominis (Linnaeus Jr., 1781) RESUMO As larvas da

Cited by 3 publications

References 5 publications

Spleen cell proliferation during and after skin myiasis by human bot fly Dermatobia hominis

Spleen cell proliferation during and after skin myiasis by human bot fly Dermatobia hominis

Production of anti-Cryptosporidium polyclonal antibodies and standardization of direct immunofluorescence for detecting oocysts in water

High morbidity cutaneous enzootic myiasis by Dermatobia hominis (Diptera: Oestridae) in sambar deer (Rusa unicolor)

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