Número de cromossomos em populações de Achyrocline satureioides Lam. (marcela) do Estado do Rio Grande do Sul, Brasil

Pereira, L. P. de; Luz, Laura Patrícia da; Tedesco, Solange Bósio; Silva, Antonio Carlos Ferreira da

doi:10.1590/s0103-84782006000200052

Cited by 10 publications

(6 citation statements)

References 5 publications

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“…The four Achyrocline species studied are diploid with 2n = 28, in agreement with chromosome counts already described for the genus [Anderberg, 1994], although there is one dubious report of 2n = 24 for A. satureioides from southern Brazil [de Pereira et al, 2006]. Chromosome numbers were reported previously for A. satureioides from Argentina and Colombia [Jansen et al, 1984;Hunziker et al, 1990] and for Achyrocline alata and A. trianae from Ecuador and Peru [Turner et al, 1967;Jansen et al, 1984;Carr et al, 1999].…”

Section: Discussionsupporting

confidence: 88%

“…There are reports on chromosome numbers for A. satureioides (2n = 28, 2n = 24), A. alata (2n = 28) and A. trianae (2n = 28) and some karyological descriptions [Jansen and Stuessy, 1980;Jansen et al, 1984;Hunzinker et al, 1990;Carr et al, 1999;de Pereira et al, 2006]. The basic chromosome number x = 14 was proposed for Australian Gnaphalieae species and may also be the same for the entire tribe, but further investigation is needed [Watanabe et al, 1999].…”

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confidence: 99%

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Karyological Features of Achyrocline (Asteraceae, Gnaphalieae): Stable Karyotypes, Low DNA Content Variation and Linkage of rRNA Genes

Mazzella

Rodríguez

Vaio

et al. 2010

Cytogenet Genome Res

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Many Achyrocline (Asteraceae, tribe Gnaphalieae) species are widely used in Argentina, Brazil and Uruguay as popular medicinal and aromatic plants. Achyrocline flaccida, A. satureioides, A. alata, and A. crassiuscula are distributed in Uruguay and popularly known as ‘marcelas’. In order to characterize them, we performed chromosome counts, compared the karyotypes, mapped the 5S and 45S rDNA sites by fluorescent in situ hybridization, and estimated their DNA content. All species were diploid with 2n = 28 chromosomes, this being the first report for A. flaccida and A. crassiuscula. All species showed symmetrical karyotypes composed exclusively of biarmed chromosomes. DNA content estimated by flow cytometry revealed 2C values ranging from 5.73 to 6.03 pg, the amounts for A. alata and A. crassiuscula being higher than those for the other species. Cytogenetic mapping of 5S and 45S rDNA sequences in three species, A. flaccida, A. satureioides and A. alata, showed that in these species both sites co-localized in the pericentromeric region of chromosome 10. This site corresponds to the only DAPI^– and CMA⁺ band of their genomes. Southern blot hybridization of 5S and 45S rDNA on BamHI digested genomic DNA confirmed the tight linkage of these rDNA families into a single unit. Cytological data indicate that Achyrocline species are karyologically poorly differentiated, whereas the uncommon distribution of 5S and 45S rDNA suggests a close relationship with other genera of the Anthemidae tribe.

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Section: Discussionsupporting

confidence: 88%

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confidence: 99%

Karyological Features of Achyrocline (Asteraceae, Gnaphalieae): Stable Karyotypes, Low DNA Content Variation and Linkage of rRNA Genes

Mazzella

Rodríguez

Vaio

et al. 2010

Cytogenet Genome Res

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“…Studies of pepper chromosome number and morphology generate important data for Capsicum taxonomy (SOUZA et al, 2011), contribute to the understanding of the genetic variations involved in the evolution of the genus (MOSCONE et al, 2007), aid in the delineation of the cultivated, semi-cultivated and wild species BIANCHETTI, 2008;PEREIRA et al, 2006) and further plant diversity conservation by supplying information to aid genetic improvement programs for this genus (SOUZA et al, 2011). Rohani et al (2010) mentioned that cytogenetic studies of different Capsicum genebank accessions comprise an important data source for breeders, which allows for better gene pool administration and a more efficient selection of genetic resources.…”

Section: Introductionmentioning

confidence: 99%

Karyotypic characterization of Capsicum sp. accessions

et al. 2015

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ABSTRACT. This study aimed to determine the chromosome number and the karyotype of Capsicum annuum, Capsicum chinense, Capsicum frutencens and Capsicum baccatum accessions in the active Capsicum sp. genebank at the Federal University of Piauí (BGC-UFPI). These species have great economic importance throughout the world, and their cytogenetic characterization can inform taxonomy and lead to improvement in the genus. Karyotypes were obtained from the rootlet meristems of the studied accessions using the squash method and Giemsa staining. The chromosome number 2n=2x=24 was verified for each of the four species. Chromosomal polymorphisms were observed for the C. frutencens accession BGC 37, which presented 12 pairs of metacentric chromosomes instead of 11 pairs of metacentric chromosomes and 1 submetacentric chromosome pair present in other accessions. In the accessions BGC 01 and BGC 37, secondary constrictions were observed in the 1 and 12 as well as in the 6 and 11 homologs, respectively. The karyotypes of the studied species were asymmetrical among themselves. The results obtained in this study confirm the high genetic diversity previously described in the literature for this genus.Keywords: pepper, cytogenetics, genebank, morphometry.Caracterização cariotípica de acessos de Capsicum sp.RESUMO. Este trabalho teve por objetivo caracterizar numérica e morfologicamente os cariótipos de acessos de Capsicum annum, Capsicum chinense, Capsicum frutencens e Capsicum baccatum pertencentes à coleção do Banco ativo de Germoplasma de Capsicum sp. da Universidade Federal do Piauí (BGC-UFPI). Estas espécies têm grande importância econômica em todo mundo, e a caracterização citogenética fornece informações importantes para estudos de taxonomia bem como para programas de melhoramento genético do gênero na qual estão inseridas. Os cariótipos foram obtidos pelo método de esmagamento e coloração com Giemsa de células dos meristemas das radículas dos acessos estudados. A partir dos resultados verificou-se número cromossômico 2n = 2x = 24 para todas as quatro espécies. Foi observado polimorfismo cromossômico para o acesso BGC 37 da espécie C. frutencens, que apresentou 12 pares de cromossomos metacêntricos, diferindo dos outros que apresentaram 11 pares de cromossomos metacêntricos e 1 par de cromossomo sub-metacêntrico. Visualizou-se nos acessos BGC 01 e BGC 37 desta espécie constrições secundárias nos homólogos 1 e 12, e 6 e 11, respectivamente. Os cariótipos das espécies estudadas foram assimétricos entre si. Os resultados obtidos neste estudo corroboram com a grande diversidade genética descrita na literatura para o gênero Capsicum.Palavras-chave: pimenta, citogenética, banco de germoplasma, morfometria.

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“…Studies characterizing the germplasm of species are essential to optimize the use of native medicinal resources of a country and conserve the available plant genetic diversity (Pereira et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Antiproliferative effect of the tree and medicinal species Luehea divaricata on the Allium Cepa cell cycle

2012

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The medicinal and tree species Luehea divaricata is known as 'açoita-cavalo' and widely used for wood, reclamation, and in popular medicine. The aim of this study was to evaluate the antiproliferative and genotoxic effects of infusions of two populations of this species on the Allium cepa cell cycle. Cells of root tips of Allium cepa were used as an in vivo test system for monitoring the genotoxicity of this medicinal plant. Leaves and bark of two populations of Luehea divaricata were collected during the vegetative stage and used to prepare infusions in two concentrations: for the leaves (6 g/L and 30 g/L) and two concentrations for the bark (32 g/L and 160 g/L), using distilled water as negative control and glyphosate 3% as positive control. For this study, 10 groups of four bulbs were utilized, with one group of bulbs for each treatment. The slides were prepared by the squashing technique, scoring 4000 cells for each group of bulbs. The mitotic index (MI) was calculated and then a statistical analysis was performed using chi-square (χ 2 ). The results showed that Luehea divaricata infusions in both populations caused a reduction of MI compared to control, and in both analyzed concentrations there was no significant genotoxic effect in comparison to the negative control, however there was a significant difference in relation to the positive control for both populations. The antiproliferative effect of leaf extracts increased with a greater concentration and among bark extracts no significant difference occurred between the two concentrations. The studied populations did not show genetic variability regarding the antiproliferative effect.

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Número de cromossomos em populações de Achyrocline satureioides Lam. (marcela) do Estado do Rio Grande do Sul, Brasil

Cited by 10 publications

References 5 publications

Karyological Features of <i>Achyrocline</i> (Asteraceae, Gnaphalieae): Stable Karyotypes, Low DNA Content Variation and Linkage of rRNA Genes

Karyological Features of <i>Achyrocline</i> (Asteraceae, Gnaphalieae): Stable Karyotypes, Low DNA Content Variation and Linkage of rRNA Genes

<b>Karyotypic characterization of <i>Capsicum</i> sp. accessions

Antiproliferative effect of the tree and medicinal species Luehea divaricata on the Allium Cepa cell cycle

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