Soroprevalência e fatores de risco para a infecção pelo herpesvírus bovino tipo 1 (BHV-1) no Estado de Goiás, Brasil

Barbosa, Alessandra Cartafina Vaz da Costa; Brito, Wilia Marta Elsner Diederichsen de; Alfaia, Bernadete Tavares

doi:10.1590/s0103-84782005000600022

Cited by 19 publications

(14 citation statements)

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“…Thus, some animals seropositive to BoHV-1 may alternatively be positive for BoHV-5. Even so, the diagnostic test used in this study is considered standard for the BoHV-1 diagnosis (Rocha et al, 2001) and many authors have used this method to estimate the prevalence of BoHV-1 in different states of Brazil (Medici et al, 2000;Barbosa et al, 2005). The results of this study confirm the presence of BoHV-1 in dairy cattle herds of all studied macro-regions.…”

Section: Resultssupporting

confidence: 63%

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Antibodies against Bovine herpesvirus 1 in dairy herds in the state of Espirito Santo, Brasil

et al. 2014

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RESUMO Anticorpos contra herpesvírus bovino 1 em rebanhos leiteiros do Estado do Espírito Santo, BrasilO herpesvírus bovino 1 (Bovine herpesvirus 1 -BoHV-1) causa grandes prejuízos econômicos à pecuária bovina mundial, por comprometer os sistemas respiratório e reprodutivo de bovinos de corte e leiteiros. Nas últimas décadas, os registros de casos clínicos ocasionados pelo BoHV-1, no Brasil, mostram que têm aumentado gradualmente, o que torna importante o delineamento de estudos que mostrem a distribuição da infecção nas diversas Received:05/03/2012; Approved:19/11/2013. Bovine herpesvirus 1 (BoHV-1) causes major losses in worldwide livestock, affecting the respiratory and reproductive tracts of bovine. In the past decades, the number of cases in Brazil has been gradually increasing. Therefore, it is important to assess the distribution of infection in different regions of the country. In the state of Espírito Santo (ES) the BoHV 1 infection rate in dairy cattle herds is unknown. Thus, the aim of this study was to detect neutralizing antibodies against BoHV-1 in serum samples from 1,161 non-vaccinated cows from 59 dairy cattle herds in 23 municipalities of the Metropolitan, North, Northwest and South macro-regions. The identification of seropositive cows was evaluated by the virus neutralization test. The results showed that of all serum samples evaluated 775 (66.75%) had neutralizing antibodies against BoHV-1. Moreover, all herds were found positive; however, the percentage of positive cows varied among regions; 49.06%, 62.15%, 67.21% and 80.04% for the Metropolitan, South, North and Northwest macro-regions, respectively. In this study, the results clearly indicate the dissemination of the viral agent in dairy cattle in the ES state, requiring the monitoring and control of diseases related to BoHV-1 infection.

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Section: Resultssupporting

confidence: 63%

“…A study in the State of Goiás, Barbosa et al, (2005) reported 51.9% of infection rate. In the state of Paraná, in a study including farms with a history of reproductive problems, 41.9% of the animals and 90.5% of dairy herds were positive for BoHV-1 (Medici et al, 2000).…”

Section: Resultsmentioning

confidence: 97%

Antibodies against Bovine herpesvirus 1 in dairy herds in the state of Espirito Santo, Brasil

et al. 2014

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“…From above, it is not possible to infer whether BoHV-1, BoHV-5 or both virus types are actually prevalent in sampled cattle. Likewise, looking back into the various studies performed on BoHV-1 prevalence performed in Brazil, based solely on testing with a single BoHV-1 at SN challenge (Lovato et al 1995, Lage et al 1996, Melo et al 1997, Cerqueira et al 2000, Barbosa et al 2005 it would not be possible to measure the extent of BoHV-5 contamination of such data. So, to date, it is not possible to infer whether those reflect BoHV-1 prevalence, BoHV-5 prevalence or mixed infections.…”

Section: Discussionmentioning

confidence: 99%

Serum neutralization with different types and subtypes of bovine herpesvirus 1 and 5

Holz

Cibulski²,

Teixeira

et al. 2010

Pesq. Vet. Bras.

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The serum neutralization (SN) test is the gold standard method to measure neutralizing antibodies to bovine herpesviruses. However, in view of the further subdivisions of bovine herpesviruses in types/subtypes, defining which virus to use at challenge in SN tests may be difficult. In view of that, this study was carried out to re-evaluate (SN) sensitivity with different types/subtypes of bovine herpesviruses types 1 (BoHV-1) and 5 (BoHV-5) as challenge viruses. Bovine sera (n=810) were collected from two distinct geographic regions and tested by SN with three type 1 viruses (BoHV-1.1 strains "Los Angeles" and "EVI123/98"; BoHV-1.2a strain "SV265/96") and three type 5 viruses (BoHV-5a strain "EVI88/95"; BoHV-5b strain "A663" and BoHV-5c "ISO97/95"). SN tests were performed with a 1 hour incubation of the serum-virus mixtures at 37ºC against 100 TCID50 of each of the viruses. SN sensitivity varied greatly depending on the challenge virus used in the test. The highest sensitivity (327 positive/810 total sera tested; 40.37%) was attained when the positive results to the six viruses were added together. No association could be found between any particular type or subtype of virus and the sensitivity of the test. When positive results to each single strain were considered, SN sensitivity varied from 41.7% to 81.7%, depending on the virus and the geographic region of origin of the sera. Variation was detected even when challenge viruses belonged to the same subtype, where disagreement between positive results reached 41%. These results indicate that one hour incubation SN tests against single viruses, as performed here, may display a significantly low sensitivity (p=0.05); performing SN tests against a number of different viruses may increase considerably SN sensitivity. Furthermore, the choice of virus used for challenge is critical in SN tests. In addition, sera from different geographic regions may give rise to disagreeing results with different strains of BoHV-1 and BoHV-5. This might be particularly relevant for control programs and in international trade, were maximum sensitivity should be targeted. RESUMO.-[Soroneutralização com diferentes tipos e subtipos de herpesvírus bovinos 1e 5.] O teste de soroneutralização (SN) é o método padrão para a mensuração de anticorpos neutralizantes para herpesvírus bovinos. Entretanto, com as subdivisões propostas destes agentes em tipos e subtipos, a definição de qual amostra utilizar como virus de desafio à SN pode ser difícil. Em vista disso, este estudo foi realizado para re-avaliar a sensibilidade de testes de SN utilizando diferentes tipos e subtipos de herpesvírus bovinos tipos 1 (BoHV-1) e 5 (BoHV-5) como amostras de desafio. Soros bovinos (n=810) foram coletados de duas regiões geográficas distintas e testados frente a amostras do tipo 1 (BoHV-1.1:Pesq. Vet. Bras. 30 (7):515-522, julho 2010Carine Lidiane Holz et al. 516 amostras "Los Angeles" e "EVI123/98", BoHV-1.2a: amostra "SV265/96") e três amostras do tipo 5 (BoHV-5a: "EVI88/95"; BoHV-5b: "A663" e BoHV-5c "ISO...

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“…Um estudo envolvendo propriedades com problemas reprodutivos demonstrou a freqüência de 92,6% de rebanhos positivos (Médici et al 2000). Barbosa et al (2005) estudaram três estratos no Estado de Goiás, caracterizados pelos tipos de exploração corte, leite e misto, utilizando como base para o cálculo das amostras, o delineamento do PNCETB. Foi verificada prevalência de focos do BoHV-1 de 98,5% (I.C.95% = 97,5-99,2%), estimativa que se assemelha a estudos em propriedades com problemas reprodutivos.…”

Section: Discussionunclassified

Fatores de risco associados à infecção pelo herpesvírus bovino 1 em rebanhos bovinos da região Oeste do Estado do Paraná

et al. 2008

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The aim of this study was to determine the prevalence of positive herds and to identify the risk factors for BoHV-1 infection in breeding cattle herds in the Western region of Parana State. The statistic delineation, the serum samples and information regarding the selected farms, were the same employed in the study of bovine brucellosis for Parana State in the context of National Program for Control and Eradication of Brucellosis and Tuberculosis. The sampling was performed in two stages. Blood samples were collected from 1930 females aged ≥24 months from 295 non vaccinated herds. Serum samples were tested for antibodies against BoHV-1 using an indirect ELISA. The epidemiological questionnaire was applied on all the selected farms and aimed to obtain epidemiological data. Hundred ninety of the 295 herds were positive for BoHV-1, presenting prevalence of positive herds of 64. 41% [58.65-69.87%]. The number (≥23) of females aged 24 months (OR=2.22; IC: 1.09-4.51), purchased cattle (OR=2.68; IC: 1.48-4.82), use of common grass (OR=5.93; IC: 1.31-26.82), history of abortion in the last 12 months (OR=2.37; IC: 1.09-5.16) and presence of wildlife animals (OR=8.86; IC: 1.11-70.73) were identified as risk factors for BoHV-1 infection in the multivariate logistic regression. These results indicate that BoHV-1 infection is widespread in the studied region and that factors related to the herd characteristic and management are associated with the infection.INDEX TERMS: Prevalence, epidemiology, risk factors, bovine herpesvirus 1, indirect ELISA.

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Soroprevalência e fatores de risco para a infecção pelo herpesvírus bovino tipo 1 (BHV-1) no Estado de Goiás, Brasil

Cited by 19 publications

References 14 publications

Antibodies against Bovine herpesvirus 1 in dairy herds in the state of Espirito Santo, Brasil

Antibodies against Bovine herpesvirus 1 in dairy herds in the state of Espirito Santo, Brasil

Serum neutralization with different types and subtypes of bovine herpesvirus 1 and 5

Fatores de risco associados à infecção pelo herpesvírus bovino 1 em rebanhos bovinos da região Oeste do Estado do Paraná

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