Técnica para o estudo da anatomia da epiderme foliar de batata

Segatto, Fernanda Bastos; Bisognin, Dílson Antônio; Benedetti, Marlova; Costa, Liege Camargo da; Rampelotto, Marcos Vicente; Nicoloso, Fernando Teixeira

doi:10.1590/s0103-84782004000500042

Cited by 79 publications

(37 citation statements)

References 4 publications

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“…The number of glandular and non-glandular trichomes on these three apical leafl ets was also registered in order to correlate whitefl y oviposition with trichome density. Trichome numbers were determined using the epidermal fi ngerprint technique (Segatto et al 2004), and trichome density was calculated according to McAuslane (1996).…”

Section: Methodsmentioning

confidence: 99%

Influence of trichomes on attractiveness and ovipositional preference of Bemisia tabaci (Genn.) B biotype (Hemiptera: Aleyrodidae) on tomato genotypes

Oriani¹,

Vendramim²

2010

Neotrop. entomol.

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-Brazil is one of the world's largest tomato producer, but considerable part of the production is lost due to the attack of Bemisia tabaci (Genn.) B biotype. Resistant germoplasm plants can be an important method for B. tabaci control in integrated pest management approaches. The attractiveness and ovipositional preference of B. tabaci for 17 tomato genotypes were evaluated in a free-choice test. Trials were set up in a randomized block design with ten replicates. Each replicate (one tomato plant per pot) was placed in a cage (80 x 50 x 50 cm) and infested with 1,000 adults during four days. Linear correlation tests were applied between the number of insects and eggs and number of trichomes in each tomato genotype. LA716, LA444-1 and PI134418 genotypes were the least attractive, while the 'Santa Clara' was the most attractive; PI134417 trapped the largest number of adults. LA716 genotype (4.1 eggs/leafl et and 2.1 cm 2 /eggs per leafl et) was the least preferred for whitefl y oviposition; NAV1062, 'Fanny', LA1335, 'Santa Clara' and IAC294 were the most preferred genotypes. The glandular trichomes density was negatively correlated with whitefl y's attractiveness and oviposition per leafl et and per leaf, and positively with the number of trapped insects. The non-glandular trichomes density was negatively correlated with the number of trapped insects and positively with whitefl y's oviposition per cm 2 /leafl et and per cm 2 /leaf. LA716 had high antixenosis level (ovipositional nonpreference) toward B. tabaci B biotype related with type IV glandular trichome.

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Section: Methodsmentioning

confidence: 99%

Influence of trichomes on attractiveness and ovipositional preference of Bemisia tabaci (Genn.) B biotype (Hemiptera: Aleyrodidae) on tomato genotypes

Oriani¹,

Vendramim²

2010

Neotrop. entomol.

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“…Despite wide use, anatomical studies apply laborious techniques (Macedo, 1997) that make work hard and long time-consuming. Segatto et al (2004) demonstrated a study of leaf epidermis anatomy in potato clones (Solanum tuberosum L.) by an alternative technique, the printing out of leaf epidermis with cianoacrilate ester universal instantaneous adhesive. The aim of this work was to identify some Lauraceae tree species from remnants of Seasonal Forests at Serra Geral formation, Rio Grande do Sul state, applying the epidermical printing technique with cianocrilate ester as an alternative anatomic tool.…”

Section: Introductionmentioning

confidence: 99%

Leaf surface characters applied to Lauraceae taxonomy in a Seasonal Forest of Southern Brazil

Ceolin

Rosito²,

Canto-Dorow³

2009

Braz. arch. biol. technol.

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The goal of this work was to test if the macro and micro morphological analysis of the leaf surface could provide vegetative diagnostic characters for some of the most common Lauraceae species in Southern Brazilian Seasonal Forests. The leaf printing technique with universal instantaneous adhesive was used for the epidermical microscopical analysis and external macroscospical analyses of leave were made. Microscopic evaluation revealed the visibility and contours of anticlinal walls of epidermical cell and stomata and shape of guard-cells. Macroscopic evaluation showed the absence or presence of characters such as hairiness, domatia and scents.The results showed that analyzed characters, together with other diagnostic characteristics, could contribute in taxonomic delimitation of some common Lauraceae species in the Southern Brazil.

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“…IAC "Gomo-de-mel", cultivada in vitro a partir de gemas axilares inoculadas em meio de cultura Murashige & Skoog (1962) O número de estômatos e escamas foi determinado pelo método de impressão epidérmica (Segatto et al 2004), utilizando éster de cianoacrilato (LOCTITE ® ). As lâminas histológicas foram analisadas em microscópio de luz equipado com ocular quadriculada com área foliar de 0,78 mm acondicionadas em cada um dos ambientes (in vitro e ex vitro), totalizando 25 lâminas para cada tratamento.…”

Section: Methodsunclassified

Alterações morfológicas foliares em abacaxizeiros cv. IAC "Gomo-de-mel" micropropagados e aclimatizados em diferentes condições de luminosidade

et al. 2009

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RESUMO -(Alterações morfológicas foliares em abacaxizeiros cv. IAC "Gomo-de-mel" micropropagados e aclimatizados em diferentes condições de luminosidade). Plantas micropropagadas geralmente apresentam dificuldades de adaptação ao meio ex vitro, sendo muitas vezes submetidas a processos de rustificação para minimizar os impactos decorrentes da mudança de ambiente. Uma vez que a folha e seus anexos destacam-se como importantes indicativos das estratégias adaptativas das plantas a ambientes adversos, este trabalho teve por objetivo comparar a anatomia foliar de plantas de abacaxi cv. IAC "Gomo-de-mel" cultivadas in vitro com microplantas aclimatizadas em diferentes condições de luminosidade, sob telado com 50% de sombreamento e em pleno sol para verificar a necessidade do processo de rustificação para este cultivar. Avaliações por meio de microscopia de luz e eletrônica de varredura da epiderme foliar, demonstraram aumento na densidade de escamas em ambas as superfícies das folhas, em microplantas dos ambientes ex vitro, principalmente em relação às que foram expostas diretamente ao sol. Observou-se ainda, aumento no espessamento da cutícula, na sinuosidade das células epidérmicas, e na distribuição e quantidade de fibras no mesofilo evidenciando a interferência das condições de luminosidade nas características morfológicas das microplantas. Essas alterações não prejudicaram o desenvolvimento das microplantas, indicando que não são necessárias etapas de rustificação para a aclimatização deste cultivar. Palavras-chave: aclimatização, Ananas comosus, anexos epidérmicos, microplantasABSTRACT -(Morphological alterations in leave of micropropagated pineapple plants cv. IAC "Gomo-de-mel" acclimatizated in different conditions of luminosity). Microprapagated plants usually show difficulties to adapt to ex vitro conditions, and many times are submitted to the rustication process to aim the reduction of all the impacts resulting from the environmental changes. Once the leaf and its annexes are important indicators of adaptability strategies of the plants to adverse environmental conditions, the objective of this work was to compare the leaf anatomy of pineapple cv. IAC "Gomo-de-mel" in vitro cultivated plants with microplants acclimatized in different conditions of luminosity, under mesh, with 50 % of shading and directly exposed to sunlight, to verify the needed of rustication process on this cultivar. Evaluations of the leaf epidermis using light and electronic scanning microscopy showed an increase on scale density in both leaves surfaces of the ex vitro microplants, mainly related to the ones directly exposed to sunlight. Subsequent observations showed an increase on cuticle thickness, on wavy contours of epidermal cells, and on the distribution and quantity of mesophyll fibers, evidencing the light conditions interference in morphological characteristics of these microplants. These alterations had not harmed microplant development, showing that are not need of rustication stages on the acclimatization process of this c...

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Técnica para o estudo da anatomia da epiderme foliar de batata

Cited by 79 publications

References 4 publications

Influence of trichomes on attractiveness and ovipositional preference of Bemisia tabaci (Genn.) B biotype (Hemiptera: Aleyrodidae) on tomato genotypes

Influence of trichomes on attractiveness and ovipositional preference of Bemisia tabaci (Genn.) B biotype (Hemiptera: Aleyrodidae) on tomato genotypes

Leaf surface characters applied to Lauraceae taxonomy in a Seasonal Forest of Southern Brazil

Alterações morfológicas foliares em abacaxizeiros cv. IAC "Gomo-de-mel" micropropagados e aclimatizados em diferentes condições de luminosidade

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