Androgênese: Uma Rota Alternativa No Desenvolvimento Do Pólen

Santos, Eliane Kaltchuk dos; Zanettini, Maria Helena Bodanese

doi:10.1590/s0103-84782002000100028

Cited by 6 publications

(2 citation statements)

References 41 publications

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“…Several methods have been used to induce haploid Cucurbitaceae plants, such as the use of irradiated pollen in melons [25] and watermelons [12,22], as well as the use of gynogenesis (ova and ovaries cultured in vitro) and androgenesis (microspores and anthers cultured in vitro) [7]. One of the routes that can be followed in androgenesis lines for callus proliferation and formation [9], is via embryogenesis or organogenesis to form a new plant [21,8]. Previous studies regarding callus induction focused on efficient plant regeneration using different explant types in cucumbers [2,10] and pumpkins [20].…”

Section: Introductionmentioning

confidence: 99%

The Effect of Temperature and Growth Regulators on Callus Induction in Watermelon Anthers

Silva

Dias

Melo

2021

Braz. arch. biol. technol.

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Callus induction is one of the pathways required for haploid plant regeneration through anther culture. Pollen viability, as well as the effect of growth regulators and cold pretreatment on anthers of two watermelon lines (Smile and Sugar Baby) to induce callus formation were herein evaluated. Pollen viability was estimated through the staining technique using 2% acetic carmine. Male flower buds were collected and disinfested to allow removal anthers. These anthers were placed on Murashige and Skoog medium, which was supplemented with 2,4-dichlorophenoxyacetic (2,4-D) at 0.0, 0.5, 1.0, 2.0 or 5.0 μM or with 6benzylaminopurine at 0.0, 0.5, 1.0, 1.5, or 2.0 μM, in combination with 2.0 μM of 2,4-dichlorophenoxyacetic. Anthers were pretreated at 4 °C, for two days and then placed in vitro. Both watermelon lines provided high pollen viability rates (from 93 to 98%). The 2.0 and 5.0 μM concentrations of 2,4-D stimulated higher friable callus formation. The optimal concentration of 2,4-D was estimated at 3.78 μM and 4.17 μM, which had callus induction rates of 64% and 52%, respectively. The combination of 2.0 μM of 2,4-D and 6benzylaminopurine did not lead to increased anther response to callus induction. The pre-treatment applied to flower buds at 4 °C enabled callus induction and the anther response to callus induction was genotypedependent.

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Section: Introductionmentioning

confidence: 99%

The Effect of Temperature and Growth Regulators on Callus Induction in Watermelon Anthers

Silva

Dias

Melo

2021

Braz. arch. biol. technol.

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“…Além de ser um processo demorado e trabalhoso, a eficiência da seleção nas primeiras gerações de autofecundação é muito baixa, devido, principalmente, à ocorrência de alelos dominantes em heterozigose (KALTCHUK-SANTOS & BODANESE-ZANETTINI, 2002).…”

Section: Introductionunclassified

Biotecnologia aplicada ao melhoramento genético do cafeeiro

Morais

Melo

2011

Cienc. Rural

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Ultrastructure and germination of Vitis vinifera cv. Loureiro pollen

Abreu¹,

Costa²,

Oliveira³

et al. 2006

Protoplasma

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The cultivar Loureiro of Vitis vinifera is one of the most economically important, recommended in almost the totality of the Região Demarcada dos Vinhos Verdes. In vineyards, the grape productivity of this cultivar is normal while in others it is extremely low. The aim of this work was to study the morphology and germination of Vitis vinifera cv. Loureiro pollen with high and low productivity. The pollen grain was examined under light, transmission and scanning electron microscopy. Typically V. vinifera pollen present three furrows but in the cultivar Loureiro we found tricolporated and acolporated (without furrows or pores) pollen grains. Both pollen types present generative and vegetative cells with the usual aspect and a dense cytoplasm rich in organelles. In the acolporated pollen a continuous exine layer and an irregular intine layer were observed. Differences were found in the starch accumulation, since only in tricolporated pollen abundant plastids filled with numerous starch granules were observed. To determine the causes of the low productivity of this cultivar we tested pollen viability by the fluorochromatic reaction and pollen germinability by in vitro assays. We observed that the acolporated pollen grain is viable, but no germination was recorded.

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Androgênese: Uma Rota Alternativa No Desenvolvimento Do Pólen

Abstract: RESUMO A androgênese é o fênomeno no qual um grão de

Cited by 6 publications

References 41 publications

The Effect of Temperature and Growth Regulators on Callus Induction in Watermelon Anthers

The Effect of Temperature and Growth Regulators on Callus Induction in Watermelon Anthers

Biotecnologia aplicada ao melhoramento genético do cafeeiro

Ultrastructure and germination of Vitis vinifera cv. Loureiro pollen

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