The cultivar Loureiro of Vitis vinifera is one of the most economically important, recommended in almost the totality of the Região Demarcada dos Vinhos Verdes. In vineyards, the grape productivity of this cultivar is normal while in others it is extremely low. The aim of this work was to study the morphology and germination of Vitis vinifera cv. Loureiro pollen with high and low productivity. The pollen grain was examined under light, transmission and scanning electron microscopy. Typically V. vinifera pollen present three furrows but in the cultivar Loureiro we found tricolporated and acolporated (without furrows or pores) pollen grains. Both pollen types present generative and vegetative cells with the usual aspect and a dense cytoplasm rich in organelles. In the acolporated pollen a continuous exine layer and an irregular intine layer were observed. Differences were found in the starch accumulation, since only in tricolporated pollen abundant plastids filled with numerous starch granules were observed. To determine the causes of the low productivity of this cultivar we tested pollen viability by the fluorochromatic reaction and pollen germinability by in vitro assays. We observed that the acolporated pollen grain is viable, but no germination was recorded.
The causes of fruit abortion, in eggplants, may be related to the absence of viable pollen under high temperatures, common in the Northeast region of Brazil, resulting in a reduction in the number of fruits per plant and consequently in productivity. The objective of this study was to evaluate the techniques of identification of PV – pollen viability in eggplant, as well as to correlate these results with the FFI – Fruit Fixation Index, NFP – Number of Fruits per Plant and PP – Production per Plant obtained in cultivation under high temperatures. The experiment was conducted at the Federal Rural University of Pernambuco, Recife, Brazil, between September and December 2017. The experiment design was a randomized block design with four replications, in the 7 x 4 factorial scheme (7 genotypes x 4 evaluation of PV) containing four plants per experimental plot. The results showed that the different techniques used detected viable pollens in the genotypes evaluated in smaller and in larger proportions. The highest percentages were observed in the treatments with CA – Carmine Acetic and AS – Alexander Solution and these overestimated the PV (>90%). In the test with TTC – 2,3,5-triphenyltetrazolium chloride (25%), the viability estimation was equivalent to the in vitro germination test, however the genetic correlations were null and/or very low between these techniques and the FFI, NFP and PP traits. The genetic correlations between the results obtained with CA and AS with FFI, NFP and PP under conditions of high temperatures were high and positive and suggest that the selection based on PV only, except with the use of TTC solution, may be efficient for indirect selection of genotypes with high FFI, NFP and PP.
In the northeast of Brazil, the yield of eggplant has been unpredictable, especially when the flowering coincides with the hottest period of the year. The objective of this study was to evaluate eggplant genotypes for tolerance to high temperatures and to identify correlations between traits that aid the indirect selection of genotypes tolerant to high temperatures. Twenty-two genotypes were arranged in a randomized block design with four replications conducted in a greenhouse and in the open field, both located at the Universidade Federal Rural de Pernambuco, Recife, Pernambuco, Brazil, between December 2016 and May 2017. Positive correlations were obtained for the pairs, number of fruits per plant (NFP) x fruit fixation index (FFI), NFP x production per plant (PP) and PP x FFI and negative for the pair NFP x PP. The associations among the traits pollen viability (PV), FFI, NFP and PP were low and/or negative for all pairs in both environments and indicates that the indirect selection for FFI and PP through PV is not efficient. Higher values for PV, NFP, PP were observed in greenhouse cultivation, while in the field the genotypes had the best performance for fruit weight (FWe) FFI, fruit length (FL), fruit width (FWi) and length/width ratio of fruit (FLWR). In high temperature conditions, the genotypes CNPH 135, CNPH 93, CNPH 79, CNPH 84, CNPH 71, CNPH 71, CNPH 668, Ajimurasaki F1 and Kokushi Onaga F1 with good FFI and CNPH 135 with the highest FFI, PP, PV and PWe. The FFI in 45.4% of the genotypes under high temperatures was low, around 21.3 and 40.5%. In the field, genotypes CNPH 84 and CNPH 668 stood out with the best FFI (> 60%).
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