Lesões de isquemia e reperfusão no intestino de eqüinos: fisiopatologia e terapêutica

Alves, Geraldo Eleno Silveira; Faleiros, Rafael Resende; Júnior, Antônio de Pinho Marques

doi:10.1590/s0103-84782000000600028

Cited by 3 publications

(1 citation statement)

References 47 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Em humanos e equinos submetidos a cirurgias intestinais, a probabilidade de desenvolver íleo adinâmico durante o período pós-operatório é alta e nos equinos, quanto maior essa probabilidade maior a taxa de óbito (Matos et al 2000, Cerqueira et al 2005, Faleiros et al 2008. Sua fisiopatologia é, ainda, pouco elucidada e vários agentes etiológicos têm sido implicados, tais como distensão, isquemia, desequilíbrio entre os componentes simpático e parassimpático, dor após procedimentos cirúrgicos, endotoxemia, peritonite, distúrbios hidroeletrolíticos, hipoalbuminemia e inflamação (Koenig & Cote 2006).…”

Section: Introductionunclassified

Alterações morfométricas no plexo mioentérico do cólon menor equino distendido experimentalmente

Mendes

Escobar

Vasconcelos³

et al. 2009

Pesq. Vet. Bras.

View full text Add to dashboard Cite

RESUMO.-O cólon menor dos equinos é frequentemente acometido por afecções obstrutivas, sendo a disfunção da motilidade uma complicação comum após o tratamento cirúrgico. Este transtorno pode estar relacionado com lesões no plexo mioentérico ocorridas durante a distensão intestinal, contudo pouco se sabe sobre sua fisiopatologia. O objetivo deste estudo foi avaliar as alterações morfológi-cas na inervação mioentérica em segmentos de cólon menor de eqüinos submetidos à distensão intraluminal com pressão suficiente para promover redução da perfusão microvascular (isquemia parcial) da parede intestinal. Nove eqüinos foram submetidos à distensão do cólon menor por 4h. Fragmentos da parede intestinal foram colhidos antes e ao final da distensão, após 1,5 e 12 horas de reperfusão no segmento experimental e ao final do procedimento em The equine small colon is frequently affected by obstruction, and intestinal motility dysfunction is a common complication after its surgical treatment. This fact may be related to myoenteric plexus lesion caused by distention; however, little is known about the pathophysiology of this condition. The objective of this study was to evaluate the morphological alterations in the myoenteric inervation of segments of small colon of horses subjected to intraluminal distension with reduction of the microvascular perfusion (partial ischemia) of the intestinal wall. Nine horses were used to promote distension of on segment of small colon for 4 hours. Samples of intestinal wall were collected before and at the end of the distension, after 1.5 and 12 hours of reperfusion in the experimental segment and at the end of the procedure in a different distant segment. Samples were processed and histological sections were stained with cresyl violet for the morphometric studies. An image analyzer software was used to measure perimeter, diameter, and area of the neuronal body, nucleus and nucleolus of the neurons and the areas of the cytoplasm and nucleoplasm. Significant reductions (P<0.05) in the areas of the neuronal body and cytoplasm were detected at the end of intestinal distension, returning to the basal values during the reperfusion. In conclusion, intraluminal distension promoted changes in the morphology of the neurons of myoenteric plexus. These morphological modifications may be associated to the motility dysfunction frequently observed in clinical cases.INDEX TERMS: Small colon, distension, myoenteric plexus, horse.

show abstract

Section: Introductionunclassified

Alterações morfométricas no plexo mioentérico do cólon menor equino distendido experimentalmente

Mendes

Escobar

Vasconcelos³

et al. 2009

Pesq. Vet. Bras.

View full text Add to dashboard Cite

show abstract

Colocolic Intussusception Associated with Octangium sp. (Digenea: Microscaphidiidae) in a Green Sea Turtle Chelonia mydas

et al. 2021

View full text Add to dashboard Cite

Reports of intussusception in sea turtles are generally linked to marine debris ingestion; therefore, only a few cases of the disease are associated with parasitic infestations. The objective of this study was to describe the necropsy findings of the first reported case of colocolic intussusception in a green sea turtle Chelonia mydas, associated with the helminth Octangium sp. A juvenile female green sea turtle, which was registered and rescued by the team from the Cetaceans Project of Costa Branca, State University of Rio Grande do Norte, was examined. The animal died 1 d after being treated and was immediately submitted for necropsy. Our findings indicated that parasitic infestation by Octangium sp. in the green sea turtle caused intussusception and consequently led to the animal’s death. Early diagnosis and surgical correction are fundamental for a good prognosis and, consequently, for successful rehabilitation of marine species.

show abstract

Apoptose no cólon menor eqüino submetido à isquemia e reperfusão experimentais

Mendes

Faleiros²,

Vasconcelos³

et al. 2009

Pesq. Vet. Bras.

View full text Add to dashboard Cite

RESUMO.-Isquemia e reperfusão intestinais são importantes fatores de mortalidade em eqüinos. O objetivo deste estudo foi detectar e quantificar a apoptose na mucosa do cólon menor eqüino em um modelo de isquemia e reperfusão. Realizou-se a exposição cirúrgica do cólon menor de doze eqüinos e demarcaram-se dois segmentos intestinais que foram submetidos a 90 (SI) ou 180 (SII) minutos de isquemia arteriovenosa completa. Foram coletadas amostras intestinais antes da isquemia (controle), ao seu final e após 90 e 180 minutos de reperfusão. As amostras foram processadas histologicamente e coradas pela Hematoxilina e Eosina (SI e SII) e pela técnica de TUNEL (SII). Foram digitalizadas imagens histológi-cas e procedeu-se análise morfométrica para detectar ocorrência de apoptose e determinar o índice apoptótico Intestinal ischemia and reperfusion are important factors for mortality in horses. The objective of this study was to detect and to quantify apoptosis in the mucosa of equine small colon in a model of ischemia and reperfusion. The small colon was surgically exposed in twelve horses, and two intestinal segments were demarcated and subjected to 90 (SI) or 180 (SII) minutes of complete arteriovenous ischemia. Intestinal samples were collected before ischemia (control), at its end and after 90 and 180 minutes of reperfusion. Samples were histological processed and stained by hematoxylin and eosin (SI and SII) and by the technique of TUNEL (SI). Digitized histological images were analyzed morphometrically to detect apoptotic cells and to determine the apoptotic index (AI). After 90 or 180 minutes of arteriovenous ischemia, an increase in apoptotic cells was verified when compared with the control group, although no difference could be detected between the different periods of ischemia (P<0.05). After the first 90 minutes of reperfusion, a decrease in AI occurred, similar in both segments, possibly due to lack of energy source promoted by ischemia. AI was maximized after 180 minutes of reperfusion (sample harvested only in SI) (P<0.05). In conclusion, apoptosis is an important cause of cellular mucosal death in equine small colon ischemic obstruction, occurring early in ischemia, and later (after 90 minutes) in the reperfusion period.INDEX TERMS: Horse, small colon, apoptosis, ischemia and reperfusion.

show abstract

Lesões de isquemia e reperfusão no intestino de eqüinos: fisiopatologia e terapêutica

Cited by 3 publications

References 47 publications

Alterações morfométricas no plexo mioentérico do cólon menor equino distendido experimentalmente

Alterações morfométricas no plexo mioentérico do cólon menor equino distendido experimentalmente

Colocolic Intussusception Associated with Octangium sp. (Digenea: Microscaphidiidae) in a Green Sea Turtle Chelonia mydas

Apoptose no cólon menor eqüino submetido à isquemia e reperfusão experimentais

Contact Info

Product

Resources

About