Avaliação da viabilidade de intestino delgado com isquemia experimental pelo uso de fluoresceína sódica em eqüinos

Abstract

Com o objetivo de testar o sal sódico de fluoresceína na indicação da viabilidade intestinal em casos de processos isquêmicos do intestino delgado de eqüinos, desenvolveu-se um modelo isquêmico através de ligadura artério-venosa durante 120 minutos. Foram utilizados 10 animais, SRD, sendo seis machos, com peso e idade médios de 293kg e 11 anos. Cada animal foi submetido a duas cirurgias, uma para a realização das ligaduras e observação do desempenho da fluoresceína e outra, 24 horas após, para confirmação macroscópica das informações obtidas na primeira cirurgia. O acesso adotado foi pela linha média, sendo exteriorizado um segmento de aproximadamente 20 cm de intestino delgado. Após o período estabelecido para a isquemia, as ligaduras foram desfeitas e o segmento intestinal foi avaliado através da fluoresceína sódica, aplicada sistematicamente na dose de 100 mg/kg, quanto às condições de viabilidade tecidual. A fluoresceína sódica mostrou-se como um objetivo e seguro método de indicação da viabilidade intestinal.

Sodium fluorescein as an internal tracer on the location of bovine urine patches in pastures

“…The concentrations of SF which were located without rehydration until 50 h after application (1 × 10 −1 g L −1 and 1 × 10 −3 g L −1 ) were much lower than those used by Sampaio and Pippi (). The fact that these concentrations could be detected up to 50 h after application may be associated with a low degradation rate of SF by sunlight, as the tracer was applied in the early evening (18:00 h).…”

“…Despite dilution in the rumen and partial metabolism into monoglucuronide in the liver, the SF dosage of 50 mg kg −1 LW allowed the location of urination sites up to 34 h after administration and was apparently safe to the animals. This dose was 50% lower than the one previously used by Sampaio and Pippi () and, considering these aspects, the dose was chosen to be evaluated in the validation trial, using animals on pasture.…”

“…It was carried out in October of 2012 (end of dry season). The dose (100 mg kg −1 LW) used by Sampaio and Pippi () was considered to be equivalent to 0·3 g L −1 , considering a 300‐kg horse used by the researchers and assuming 70% water in live weight, homogeneous dispersion of SF in body water and no incorporation of SF in body tissues. Predicting that animals would receive the tracer for at least 2 d in the last trial, very low concentrations of FS were first tested.…”

“…It was carried out in November of 2012 (rainy season) at Embrapa's Dairy Research Farm. Five doses of SF, including the dose used by Sampaio and Pippi (), were tested: 50, 100, 150, 200 and 298·4 mg kg −1 LW. One‐dose ‘capsules’ of SF were prepared in advance using an internal layer of interfolded towel paper and an external layer of natural Kraft paper.…”

“…The use of SF has been reported in experiments involving many animal species. Sampaio and Pippi () tested SF as an alternative method for the evaluation of tissue viability in ischaemic duodenum, injecting a horse intravenously with a 5% SF solution, at the dose of 100 mg kg −1 LW. For the detection of urination sites by cats, Neilson () recommended administration orally of 50 mL per animal or injection of 0·3 mL per animal of a 10% SF solution and posterior location with a black‐light flashlight.…”