Effectiveness of mechanical brushing with different denture cleansing agents in reducing in vitro Candida albicans biofilm viability

Abstract

The adhesion of Candida albicans to surfaces is the prerequisite for occurrence of denture stomatitis, a common disease diagnosed among denture wearers. A routine of denture cleansing is essential to prevent biofilm formation and the onset of this infection. The aim of this study was to investigate the effectiveness of combining brushing and cleansing agents in killing C. albicans biofilm. Disks of acrylic resin were made, sterilized, and inoculated with C. albicans (10(7) cfu/mL). After incubation (37°C/48 h), specimens were randomly assigned to 10 experimental groups (n=9): 5 subjected to brushing with distilled water or cleansing agents - dentifrice slurry, 2% chlorhexidine gluconate (CHX), 1% sodium hypochlorite (NaOCl), and Polident fresh cleanse(®) (combined method) - and 4 exposed to the cleansing agents without brushing (immersion). Non-cleansed specimens were used as positive controls. The viability of cells was evaluated by XTT reduction method. Results were analyzed by Mann-Whitney and Kruskal-Wallis tests (α=0.05). The combined method was significantly more effective (p<0.0001) in reducing biofilm viability than the immersion. Brushing with CHX and NaOCl resulted in 100% removal of the biofilm. Immersion in the agents reduced significantly (p<0.0001) the biofilm viability, with CHX being the most effective (p<0.0001). The use of the combined method of brushing with cleansing agents is an effective method to reduce C. albicans biofilm, being CHX and NaOCl the most effective solutions.

Status of Removable Dentures and Relationship with Oral Candida‐Associated Factors in a Geriatric Population in Catalonia

“…These prosthetic status and maintenance findings suggest that it might be advisable to conduct periodic reviews among the institutionalized elderly to assess any denture treatment requests and to ensure that nursing home staff participate in improving oral and denture hygiene among their patients. The removable denture should be brushed daily to remove biofilm 34 and checked frequently by the dentist to help ensure oral and general health. 30 To improve preventive measures the practitioner should be aware of the prevalence of Candida colonization in denture wearers and of the factors playing major roles in promoting this colonization.…”

Evaluation of the Antifungal Effect Vitis vinifera Extract on Candida albicans

“…Most of the individuals infected are women and elderly and the adherence to the Candida albicans to the prosthesis superficies is the first step to develop this lesion. 6 Are observed tissues changes, especially on the presence of superior prosthesis, among which are found the lesions on the palate and changes on the soft tissue, accompanied or not for others inflammatory manifestations (angular queilite and glossitis). Furthermore, some patients are presented as asymptomatic to the infection; however, they normally report various symptoms, as pain, swelling, xerostomia, halitosis and bleeding, symptoms that many times impossible the prosthesis use.…”

Efeito antimicrobiano residual e citotoxidade in vitro de resina acrílica para base de prótese após imersão prolongada em agentes de limpeza

“…Quando a citotoxicidade foi avaliada por meio do efeito de hipoclorito de sódio sobre a síntese de DNA, uma inibição significativa e progressiva foi observada com concentrações de 0,0005%. ALTIERI et al, 2012;MIMA et al, 2008;PELLIZZARO et al, 2012).…”

“…Os agentes de limpeza são bem aceitos por usuários de próteses removíveis, pois são simples de manusear, acessíveis, possuem uma boa relação custo-benefício, sendo também adequados para pacientes institucionalizados (DE ANDRADE et al, 2012;PAVARINA et al, 2003;WEBB et al, 2005). Dentre as soluções utilizadas para limpeza e desinfecção de próteses totais, destacam-se o hipoclorito de sódio e o digluconato de clorexidina, que além de serem efetivos no controle do biofilme protético (DE ANDRADE et al, 2012;PELLIZZARO et al, 2012;ROSSATO et al, 2011), também são recomendados como métodos adjuntos ao tratamento da estomatite protética (BANTING;HILL, 2001;BARNABE et al, 2004;WEBB et al, 2005).…”

“…Com o objetivo de se obter um método de limpeza diário de próteses removíveis com maior biocompatibilidade aos tecidos bucais, alguns estudos têm demonstrado a efetividade antimicrobiana de soluções de hipoclorito de sódio e digluconato de clorexidina em baixas concentrações (ALTIERI et al, 2013;DA SILVA, P. M. et al, 2011;MONTAGNER et al, 2009;PELLIZZARO et al, 2012;RODRIGUEZ ACOSTA et al, 2014;WEBB et al, 2005). Entretanto, tais pesquisas não avaliaram o efeito antimicrobiano das soluções de baixas concentrações a longo prazo, simulando a rotina caseira de imersão noturna das próteses pelos pacientes (8h/dia).…”