Repair of critical-size defects with autogenous periosteum-derived cells combined with bovine anorganic apatite/collagen: an experimental study in rat calvaria

Paulo, Anderson de Oliveira; Castro-Silva, Igor Iuco; Oliveira, Davi F.; Machado, Manoel Eduardo de Lima; Bonetti-Filho, Idomeo; Granjeiro, José Mauro

doi:10.1590/s0103-64402011000400011

Cited by 24 publications

(17 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Histomorphometric analysis showed the volume density of bone tissue was about 6% or two times higher in irradiated group at 4 weeks. Considering that the normal bone repair of CSD on rat calvaria varies from 2% in 4 weeks to 4–7% in 8 weeks , our study confirms the importance of GaAlAs laser in bone biomodulation. We credited the heterogeneous results between radiographic and histomorphometric analyses to their different anatomical planes.…”

Section: Discussionsupporting

confidence: 79%

Effectiveness and acceleration of bone repair in critical‐sized rat calvarial defects using low‐level laser therapy

et al. 2013

Self Cite

View full text Add to dashboard Cite

Background and Objective Tissue regeneration remains a challenge for orthopedic and craniomaxillofacial surgery to treat bone loss. The use of low‐level laser therapy suggests a promise on this road with positive results for narrow defects. However, temporal and quantitative evaluations are required to understand the healing process of large injuries. The aim of this study was to investigate the repair of critical‐size bone defects in rat calvaria using a GaAlAs laser. Study Design/Materials and Methods Bone defects (9 mm in diameter) were created on the skull of 30 Wistar rats separated in control or irradiated group. GaAlAs laser (λ = 830 nm, energy density = 2.5 J/cm2 and output power = 50 mW) was applied after surgery and six times more at 48 hours intervals. The animals were euthanized after 2, 4, and 8 weeks. Digital radiographs, descriptive histological and histomorphometric analyses were carried out. Results Radiographic analysis showed greater bone formation in the irradiated group than control at 8 weeks, covering 45% and 28% of the defect, respectively (P < 0.05). Histological analysis showed in the irradiated groups a higher amount of bone neoformation and greater maturity at 4 and 8 weeks. Histomorphometric analysis showed that the volume density of bone tissue at 4 weeks in the irradiated group was two times higher than the control (P < 0.01). Conclusion The biomodulation of low‐level laser therapy using 830 nm wavelength light was effective in promoting bone healing in critical defects despite the unfavorable prognosis as well as it accelerated the maturation of bone tissue. Lasers Surg. Med. 46:61–67, 2014. © 2013 Wiley Periodicals, Inc.

show abstract

Section: Discussionsupporting

confidence: 79%

Effectiveness and acceleration of bone repair in critical‐sized rat calvarial defects using low‐level laser therapy

et al. 2013

Self Cite

View full text Add to dashboard Cite

show abstract

“…bioresorption, even those of natural origin 5,22 . Recently, research has been performed in the field of engineered polymer scaffolds to be used as structural bone substitutes.…”

Section: Discussionmentioning

confidence: 99%

Synthesis and cytotoxicity evaluation of granular magnesium substituted β-tricalcium phosphate

et al. 2013

Self Cite

View full text Add to dashboard Cite

Objective:The aim of this study was to produce dense granules of tricalcium phosphate (β-TCP) and magnesium (Mg) substituted β-TCP, also known as β-TCMP (Mg/Ca=0.15 mol), in order to evaluate the impact of Mg incorporation on the physicochemical parameters and in vitro biocompatibility of this novel material.Material and Methods:The materials were characterized using X-ray diffraction (XRD), infrared spectroscopy (FTIR), electron microscopy and inductively coupled plasma (ICP). Biocompatibility was assayed according to ISO 10993-12:2007 and 7405:2008, by two different tests of cell survival and integrity (XTT and CVDE).Results:The XRD profile presented the main peaks of β-TCP (JCPDS 090169) and β-TCMP (JCPDS 130404). The characteristic absorption bands of TCP were also identified by FTIR. The ICP results of β-TCMP granules extract showed a precipitation of calcium and release of Mg into the culture medium. Regarding the cytotoxicity assays, β-TCMP dense granules did not significantly affect the mitochondrial activity and relative cell density in relation to β-TCP dense granules, despite the release of Mg from granules into the cell culture medium.Conclusion:β-TCMP granules were successfully produced and were able to release Mg into media without cytotoxicity, indicating the suitability of this promising material for further biological studies on its adequacy for bone therapy.

show abstract

“…A grid of 200 points was superimposed on the images to quantify manually connective tissue, newly formed bone and biomaterial. Data were expressed as density volume (mm 2 ) according to a previously described protocol 13…”

Section: Methodsmentioning

confidence: 99%

Magnesium incorporation into β‐TCP reduced itsin vivoresorption by decreasing parathormone production

Yassuda

Costa

Fernandes

et al. 2012

J Biomedical Materials Res

View full text Add to dashboard Cite

Beta-tricalcium phosphate (β-TCP), one of the most widely used bioresorbable materials for bone therapy, can be doped with magnesium ions, generating β-TCMP. The objectives of this work were to evaluate, on a murine dental alveolus grafting model, the biocompatibility of β-TCP and β-TMCP granules by histomorphometric analysis, as well as the impact on plasmatic levels of receptor activator of nuclear factor κB ligand (RANK-L), osteoprotegerin (OPG), osteocalcin, osteopontin, and parathormone (PTH) during bone repair, using Luminex multiplexing technology. After grafting for 42 days, β-TCP grafted group presented higher bioresorption and induced more newly formed bone than β-TCMP (p < 0.05). β-TCP grafting also induced higher plasmatic levels of RANK-L, compared to β-TCMP and control (blood clot) groups at 21st day (p < 0.05). PTH, which remained at low levels in control group, presented a time-dependent increase in grafted groups, attaining significantly higher levels with β-TCP by the 42nd day (p < 0.05). RANK-L/OPG ratio increased on β-TCP group and attained a peak on the 21st day. In conclusion, β-TCP granules were more bioresorbable and osteogenic than β-TCMP granules, and the resorption of both materials might have been affected by osteoclastogenesis modulated by changes in the plasmatic levels of PTH and RANK-L.

show abstract

Repair of critical-size defects with autogenous periosteum-derived cells combined with bovine anorganic apatite/collagen: an experimental study in rat calvaria

Cited by 24 publications

References 25 publications

Effectiveness and acceleration of bone repair in critical‐sized rat calvarial defects using low‐level laser therapy

Effectiveness and acceleration of bone repair in critical‐sized rat calvarial defects using low‐level laser therapy

Synthesis and cytotoxicity evaluation of granular magnesium substituted β-tricalcium phosphate

Magnesium incorporation into β‐TCP reduced itsin vivoresorption by decreasing parathormone production

Contact Info

Product

Resources

About