Antibacterial effectiveness of peracetic acid and conventional endodontic irrigants

Abstract

This study evaluated the in vitro antibacterial activity of conventional and experimental endodontic irrigants against Enterococcus faecalis. The following substances were evaluated by direct contact test: 2.5% sodium hypochlorite (NaOCl); 2% chlorhexidine (CHX); 1% peracetic acid. After different contact periods (30 s, 1, 3, and 10 min), a neutralizing agent was applied. Serial 10-fold dilutions were prepared and plated onto tryptic soy agar (TSA) and the number of colony-forming units per milliliter (CFU/mL) was determined. Sterile saline was used as a negative control. Both 2.5% NaOCl and 2% CHX eliminated E. faecalis after 30 s of contact. Peracetic acid reduced the bacterial counts by 86% after 3 min and completely eliminated E. faecalis after 10 min. These results allow us to conclude that 1% peracetic acid is effective against E. faecalis, despite its slower action compared with 2.5% NaOCl and 2% CHX.

Do smear‐layer removal agents affect the push‐out bond strength of calcium silicate‐based endodontic sealers?

“…These findings are in accordance with Nagas et al (2012) who reported no alteration at push-out bond strength of MTA after exposure to NaOCl, EDTA and PA. These results are also in line with previous studies in which dentine treatment with several chelating agents such as EDTA, PA and CA resulted in similar smear-layer removal (Lenarda et al 2000, Lottanti et al 2009, Guerreiro-Tanomaru et al 2011, Tuncel et al 2015. In contrast, other studies reported that different chelating agents removed variable amounts of dentine ions (Span o et al 2009, Cruz-Filho et al 2011.…”

Effect of endodontic chelating solutions on the bond strength of endodontic sealers

“…23 Among several decalcifying agents available, PAA appears to be a potential alternative to EDTA to dissolve the smear layer and disinfect the root canal system. 23,24 A previous study has shown that 1% PAA had similar effect against Enterococcus faecalis compared with NaOCl and chlorhexidine. 24 PAA solution contains and liberates acetic acid, which is a weak chelating agent, but can reduce the smear layer as effective as EDTA.…”

“…23,24 A previous study has shown that 1% PAA had similar effect against Enterococcus faecalis compared with NaOCl and chlorhexidine. 24 PAA solution contains and liberates acetic acid, which is a weak chelating agent, but can reduce the smear layer as effective as EDTA. 23 The results of this study necessitates rejection of the null hypothesis, because the debonding values obtained after PAA treatment were statistically similar to those of EDTA-treated specimens.…”

Antimicrobial activity of Chlorhexidine, Peracetic acid and Sodium hypochlorite/etidronate irrigant solutions against Enterococcus faecalis biofilms

“…In addition, previous studies reported that PAA action is both time‐ and concentration‐dependent, so that longer exposure times and higher concentrations could show higher antimicrobial activity, similar that of to NaOCl (Guerreiro‐Tanomaru et al . , Ordinola‐Zapata et al . ).…”