Antimicrobial efficacy of chemical disinfectants on contaminated full metal crowns

Abstract

Prosthetic restorations that have been tried in the patient's mouth are potential sources of infection. In order to avoid cross-infection, protocols for infection control should be established in dental office and laboratory. This study evaluated the antimicrobial efficacy of disinfectants on full metal crowns contaminated with microorganisms. Full crowns cast in a Ni-Cr alloy were assigned to one control group (n=6) and 5 experimental groups (n=18). The crowns were placed in flat-bottom glass balloons and were autoclaved. A microbial suspension of each type of strain - S. aureus, P. aeruginosa, S. mutans, E. faecalis and C. albicans- was aseptically added to each experimental group, the crowns being allowed for contamination during 30 min. The contaminated specimens were placed into recipients with the chemical disinfectants (1% and 2% sodium hypochlorite and 2% glutaraldehyde) for 5, 10 and 15 min. Thereafter, the crowns were placed into tubes containing different broths and incubated at 35ºC. The control specimens were contaminated, immersed in distilled water for 20 min and cultured in Thioglycollate broth at 35ºC. Microbial growth assay was performed by qualitative visual examination after 48 h, 7 and 12 days. Microbial growth was noticed only in the control group. In the experimental groups, turbidity of the broths was not observed, regardless of the strains and immersion intervals, thus indicating absence of microbial growth. In conclusion, all chemical disinfectants were effective in preventing microbial growth onto full metal crowns.

Isolation and identification of Enterococcus faecalis from cultured Oreochromis niloticus and Mugil cephalus with a special emphasis on a possible integrated control strategy

“…The pH plays a major role in both GA and QACS activities, with the compounds acting better at a pH greater than 7 (Dvorak, 2005). Our results were consistent with Orsi et al (2010) who reported a significant increase in glutaraldehyde activity in alkaline than in acidic pH, as more reactive sites were formed on the cell surface, resulting in a rapid bactericidal effect. A further explanation is that, at low salt concentrations, a marked delay in the GA degradation rate could occur, in agreement with the previous findings of Hong (2017).…”

Comparison of Antimicrobial Activity between Chemical Disinfectants on Contaminated Orthodontic Pliers

“…8 Two percent GTA is a high-level disinfectant, used in the processing of medical/dental thermosensitive equipments, and is effective against a great variety of microorganisms. 2,7,9 It is active in the presence of organic matter and can act both as a disinfecting (30 minutes) and sterilizing (10 hours) agent. Its disadvantage is the toxicity, potentially causing skin and mucosal irritation, ulceration and burns; it may also cause irritation to the eyes and to the respiratory system.…”

Evaluation of acute toxicity of the "Orgasept" disinfectant

“…Among the means of physical origin, the most relevant is ultraviolet radiation (Zavgorodniy et al, 2019;Rodionova et al, 2020). The most common chemicals today are based on glutaraldehyde (Orsi et al, 2010;Paliy et al, 2020c), chlorine-active compounds (Paliy, 2014;Kamal et al, 2019;Paliy et al, 2019a), oxidants (Lineback et al, 2018;Paliy, 2018) and other compounds (Rutala, 1996;Rabenau et al, 2005). Disinfectants are proposed for use in low ambient temperatures (Paliy et al, 2020a).…”