Comparison between DNA obtained from buccal cells of the upper and lower gutter area

Abstract

This study compared quantitatively and qualitatively the DNA extracted from buccal cells collected from the upper or lower gutter areas. Buccal cells were collected from the upper (n=15) and lower gutter (n=15) region from 15 volunteers using a special cytobrush (Gentra), totaling 2 collections from each individual. DNA was extracted from the samples according to the manufacturer's instructions. The DNA obtained was qualitatively and quantitatively evaluated by 2 calibrated blind examiners using spectrophotometry and analysis of DNA bands (0.8% agarose gel electrophoresis). Data was statistically analyzed by one-way ANOVA (alpha=0.05). Means and standard derivation (SD) for total DNA yield from the upper and lower gutter area were 12.2 microg (12.0) and 9.4 microg (8.5), respectively (p=0.821). There was higher (p<0.05) DNA purity for the upper gutter (1.79; 0.05) when compared to lower gutter area (1.66; 0.10). Regarding to the DNA quality, no differences were observed between the 2 location sites, but all samples showed similar degree of degradation. In conclusion, it would be recommendable that buccal cells for DNA extraction be collected from the upper gutter area in the attempt to increase DNA purity.

Comparison of two storage conditions of Candida albicans for DNA extraction and analysis

“…Sample collection locations frequently do not support DNA extraction, and samples therefore often require transportation. However, this transportation should occur with minimum loss in the quality and quantity of genomic material (Nedel et al, 2009a(Nedel et al, , 2009b. When the DNA extraction cannot be immediately performed after collection and transportation, storage becomes an alternative.…”

Clinical Utility of Chromosomal Microarray Analysis of DNA from Buccal Cells: Detection of Mosaicism in Three Patients

“…Although blood leukocytes have long been considered the "gold standard" sample for genetic testing, there are many benefits to sample collection via buccal swab. One main benefit is that buccal collection is a non-invasive procedure that does not require use of needles or skin biopsy (Nedel et al 2009). This can be especially important for patients who do not easily tolerate blood draws or other procedures.…”