Biocompatibility of acetazolamide pastes in the subcutaneous tissue of rats

Mori, Graziela Garrido; Moraes, Ivaldo Gomes de; Nunes, Daniele Clapes; Castilho, Lithiene Ribeiro; Poi, Wilson Roberto

doi:10.1590/s0103-64402009000100002

Cited by 5 publications

(8 citation statements)

References 12 publications

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“…To perform the surgery, the animals were anesthetized with an intramuscular injection of ketamine hydrochloride (Dopalen Sespo Indústria e Comércio Ltda, Jacareí, SP, Brazil) and xylazine hydrochloride (Anasedan Agribrands do Brasil Ltda., Jacareí, SP, Brazil) at a dose of 0.05 mL/100 g body weight for each drug 18,19 . After this, the animals were divided into 3 groups: a.…”

Section: Animal Protocolmentioning

confidence: 99%

“…After preparing the Ca 3 SiO 5 (Biodentine™, Septodont, St-Maur-des-Fossés, France) according to the manufacturer's recommendations, the matrix was filled with this material. After the initial curing of the material, the specimen was introduced into the subcutaneous tissue 18 ; b. Group B: positive control (n = 8): A single dose of cyclophosphamide (Genuxal, Baxter Oncology GmbH, Halle/Westfalen, Germany) (50 mg/kg) was subcutaneously injected on the first day of the experiment 17 .…”

Section: Animal Protocolmentioning

confidence: 99%

“…Group B: positive control (n = 8): A single dose of cyclophosphamide (Genuxal, Baxter Oncology GmbH, Halle/Westfalen, Germany) (50 mg/kg) was subcutaneously injected on the first day of the experiment 17 . c. Group C: negative control (n = 8): Polypropylene tubes measuring 2 mm in diameter x 10 mm in length were subcutaneously implanted into the dorsum 18 .…”

Section: Animal Protocolmentioning

confidence: 99%

“…Lateral to the incision, the cutaneous tissue was pinched, and the tissue was dissected using blunt-ended scissors to introduce the tubes. The incisions were sutured using 4-0 nylon (Ethicon Johnson & Johnson, São Paulo, SP, Brazil) 18 .…”

Section: Animal Protocolmentioning

confidence: 99%

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Evaluation of the genotoxicity and mutagenicity of Ca3SiO5-based cement

et al. 2016

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Ca 3 SiO 5 is new cement based on the composition of Portland that has been developed to have superior physicochemical and biological properties. In a clinical evaluation, the cement did not appear to have cytotoxic properties and allowed for the proliferation of pulp cells and gingival fibroblasts. However, no previous studies have evaluated the genotoxicity or the mutagenicity of Ca 3 SiO 5 in vivo. Therefore, the goal of this study is to evaluate the genotoxic and mutagenic potential of Ca 3 SiO 5 -based cement in vivo. Twenty-four male Wistar rats were divided into 3 groups (n = 8). Group A rats received subcutaneous implantation of Ca 3 SiO 5 in the dorsum. Group B rats received a single dose of cyclophosphamide (positive control). Group C rats received subcutaneous implantation of empty tubes in the dorsum (negative control). After 24 hours, all animals were euthanized and the bone marrow of the femurs was collected for use in the comet assay and the micronucleus test. The comet assay revealed that the Ca 3 SiO 5 group had a tail intensity of 23.57 ± 7.70%, the cyclophosphamide group had a tail intensity of 27.43 ± 7.40%, and the negative control group had a tail intensity of 24.75 ± 5.55%. The average number of micronuclei was 6.25 (standard deviation, SD = 3.53) in the Ca 3 SiO 5 group, 9.75 (SD = 2.49) in the cyclophosphamide group, and 0.75 (SD = 1.03) in the negative control group. There was an increase in the micronuclei frequency in the Ca 3 SiO 5 group compared to that of the negative control group (p < 0.05). Our data showed that exposure to the Ca 3 SiO 5 -based cement resulted in an increase in the frequency of micronuclei, but no genotoxicity was detected according to the comet assay.

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Section: Animal Protocolmentioning

confidence: 99%

Section: Animal Protocolmentioning

confidence: 99%

Section: Animal Protocolmentioning

confidence: 99%

Section: Animal Protocolmentioning

confidence: 99%

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Evaluation of the genotoxicity and mutagenicity of Ca3SiO5-based cement

et al. 2016

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“…Mori et al . [ 7 ] used rat subcutaneous tissue to evaluate the biocompatibility of 2 experimental acetazolamide pastes, with physiological solution and propylene glycol used as vehicles. The experimental paste used with the physiological solution was biocompatible.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of intracanal acetazolamide in late reimplanted rat teeth

et al. 2017

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Objective:The aim of this study was to evaluate the use of acetazolamide combined with different agents as intracanal medication in late reimplanted rat teeth.Materials and Methods:In 100 Wistar rats, divided into 5 groups of 20, one of the following medications was used: Acetazolamide liquid (AL); AL with calcium hydroxide powder (ALHC); acetazolamide powder with AL; acetazolamide powder with physiological solution; and calcium hydroxide with physiological solution (control). At 30 and 60 days after reimplantation, the animals were sacrificed, tissues were processed, and cuts were stained with hematoxylin and eosin. An optical microscope was used to determine the following: percentage of inflammatory root resorption (RRI); percentage of substitute root resorption (RRS); and presence of ankylosis. The data obtained was submitted for statistical analysis.Results:Group ALHC had a significantly higher RRS than the control group at 60 days (P = 0.01). Group AL showed significantly less ankylosis than the other groups, including the control, at 30 days. AL showed results similar to those of the control group with respect to RRS.Conclusion:Acetazolamide has the potential to be an effective intracanal medication.

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