Antimicrobial activity of different concentrations of NaOCl and chlorhexidine using a contact test

Sassone, Luciana Moura; Fidel, Rivail Antônio Sérgio; Fidel, Sandra Rivera; Dias, Margarida; Hirata, Raphael

doi:10.1590/s0103-64402003000200005

Cited by 41 publications

(35 citation statements)

References 16 publications

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“…Chlorhexidine effectively eliminates E. faecalis in vitro (12)(13)(14)(15). However, it may be difficult to compare the results from different studies because of differences in study design.…”

Section: Discussionmentioning

confidence: 99%

Killing of Enterococcus faecalis by MTAD and Chlorhexidine Digluconate with or without Cetrimide in the Presence or Absence of Dentine Powder or BSA

Portenier

Waltimo

Ørstavik

et al. 2006

Journal of Endodontics

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Section: Discussionmentioning

confidence: 99%

Killing of Enterococcus faecalis by MTAD and Chlorhexidine Digluconate with or without Cetrimide in the Presence or Absence of Dentine Powder or BSA

Portenier

Waltimo

Ørstavik

et al. 2006

Journal of Endodontics

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“…However, most of researchers applied the CHX for 60 seconds. 19 In a study conducted by Sassone et al, 27 the antimicrobial effects of different concentrations of CHX (0.12, 0.5, and 1%) after immediate, 5-, 15-, and 30-minute applications were evaluated. They found that 0.12% CHX did not eliminate Enterococcus faecalis at any time interval and therefore recommended using CHX at a concentration greater than 0.12%.…”

Section: Antimicrobial Effectivenessmentioning

confidence: 99%

“…27,63,64 Vianna et al 65 found the application of 5.25% NaOCl solution for 15 seconds to eliminate Staphylococcus aureus, Candida albicans, Porphyromonas endodontalis, Porphyromonas gingivalis, and Prevotella intermedia. However, the antimicrobial activity of NaOCl has been reported to be affected by the concentration of the solution.…”

Section: Antimicrobial Effectivenessmentioning

confidence: 99%

Effects and Effectiveness of Cavity Disinfectants in Operative Dentistry: A Literature Review

Bin-Shuwaish¹

2016

The Journal of Contemporary Dental Practice

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The degree of success in the elimination of bacteria during cavity preparation and prior to the insertion of a restoration may increase the longevity of the restoration and therefore the success of the restorative procedure. The complete eradication of bacteria in a caries-affected tooth, during cavity preparation, is considered a difficult clinical task. In addition to weakening the tooth structure, attempts to excavate extensive carious tissue completely, by only mechanical procedures, may affect the vitality of the pulp. Therefore, disinfection of the cavity preparation after caries excavation can aid in the elimination of bacterial remnants that can be responsible for recurrent caries, postoperative sensitivity, and failure of the restoration. However, the effects of disinfectants on the restorative treatment have been a major concern for dental clinicians and researchers. This review aims to explore existing literature and provide information about different materials and techniques that have been used for disinfecting cavity preparations and their effects and effectiveness in operative dentistry and, therefore, helps dental practitioners with clini cal decision to use cavity disinfectants during restorative procedures. Antimicrobial effectiveness and effects on the pulp and dental restorations, in addition to possible side effects, were all reviewed in this paper.

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“…Ten experiments for each of the solutions in accordance with Crémieux and Fleurette method were performed (27). For this analysis, 1mL of the bacterial suspension was kept in contact during 5 minutes and 30 minutes under continuous stirring at 180 rpm with 1mL of each solution (EG or the respective controls).…”

Section: Antibacterial Testsmentioning

confidence: 99%

Antibacterial Efficacy of a Dispersion of Silver Nanoparticles in Citrate Medium for the Treatment of E. faecalis: an In Vitro Study

Rodríguez

Ramírez-Mora

Montero

et al. 2016

Odovtos - Int J Dent Sc

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The purpose of this study was to measure the antibacterial efficacy of a dispersion of silver nanoparticles (AgNP) in a citrate medium tested in two E. faecalis strains. AgNP were synthesized, and AgNP citrate medium (AgNP-CM) dispersion was prepared at a concentration of 100 μg/mL. The antibacterial efficacy of AgNP-CM dispersion was evaluated over two E. faecalis strains: ATCC29212 and a wild strain collected from human necrotic teeth. 5% sodium hypochlorite (NaOCl) and sterile saline solution were used as positive and negative controls. 5 and 30-minute contact tests were conducted and each experimental group were replicated 10 times. After 24 hours of incubation, the Log CFU/ mL were calculated. The AgNP obtained showed spherical shapes and had 30-60nm size. 5% NaOCl was able to completely eliminate both E. faecalis strains in all groups, showing a significant statistical difference when compared to AgNP-CM dispersion and negative control groups. AgNP-CM dispersion showed a statistically significant decrease in Log CFU/mL averages (p=0,0006) when compared to the sterile saline solution for the ATCC29212 strain during the 30-minute time. Between the 5-minute and 30-minute groups, a significant bacterial count decrease was also observed (p= 0,0128). The antibacterial efficacy of the dispersion was greater for the ATCC29212 strain than the wild strain, were the effect diminished. AgNP-CM dispersion showed a significantly lower antibacterial efficacy against E. faecalis than the 5% NaOCl at the tested times. RESUMENEl propósito del presente estudio fue medir la eficacia antibacteriana de una dispersión de nanopartículas de plata (AgNP) en medio de citrato ante dos cepas de E. faecalis. Las AgNP fueron sintetizadas y se preparó una dispersión en medio de citrato (AgNP-CM) en una concentración de 100 μg/mL. La eficacia antibacteriana de la dispersión de AgNP-CM fue evaluada ante dos cepas de E. faecalis: la ATCC29212 pura y una cepa silvestre aislada de un tratamiento endodóntico fallido. Se utilizó hipoclorito de sodio al 5% de concentración (NaOCl) y solución salina estéril como controles positivos y negativos. Se realizaron pruebas de contacto durante 5 y 30 minutos y cada grupo experimental se replicó 10 veces. Después de incubar las muestras durante 24 horas, se calcularon las concentraciones Log CFU/mL. Las AgNP obtenidas mostraron formas esféricas y un tamaño entre los 30 y 60nm. El NaOCl 5% logró eliminar por completo las cepas bacterianas en todos los grupos, mostrando una diferencia estadísticamente significativa al compararse con las dispersiones de AgNP-CM y los controles negativos. La dispersión de AgNP-CM mostró una disminución estadísticamente significativa en los promedios Log CFU/mL al compararse con la solución salina estéril para la cepa ATCC29212 a los 30 minutos de contacto. Entre los grupos de 5 y 30 minutos de la dispersión, se observó también una disminución en el conteo bacteriano (p=0,0128). La eficacia antibacteriana de la dispersión fue mayor para la cepa ATCC29212 que para la c...

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Antimicrobial activity of different concentrations of NaOCl and chlorhexidine using a contact test

Cited by 41 publications

References 16 publications

Killing of Enterococcus faecalis by MTAD and Chlorhexidine Digluconate with or without Cetrimide in the Presence or Absence of Dentine Powder or BSA

Killing of Enterococcus faecalis by MTAD and Chlorhexidine Digluconate with or without Cetrimide in the Presence or Absence of Dentine Powder or BSA

Effects and Effectiveness of Cavity Disinfectants in Operative Dentistry: A Literature Review

Antibacterial Efficacy of a Dispersion of Silver Nanoparticles in Citrate Medium for the Treatment of E. faecalis: an In Vitro Study

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