Killing of Enterococcus faecalis by MTAD and Chlorhexidine Digluconate with or without Cetrimide in the Presence or Absence of Dentine Powder or BSA

Portenier, Isabelle; Waltimo, Tuomas; Ørstavik, Dag; Haapasalo, Markus

doi:10.1016/j.joen.2005.10.027

Cited by 98 publications

(109 citation statements)

References 18 publications

(13 reference statements)

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“…3,4,15,27 In the present study, all the irrigating solutions and associations evaluated promoted elimination of planktonic microorganisms. Abdullah 30 QMiX showed similar results were observed after 5 seconds of contact. 15 The results of the present study demonstrate that the irrigating solutions present lower activity against microorganisms in biofilm, confirming the greater resistance of bacteria in this form of bacteria in this form of organization.…”

Section: 2324supporting

confidence: 73%

“…15 The results of the present study demonstrate that the irrigating solutions present lower activity against microorganisms in biofilm, confirming the greater resistance of bacteria in this form of bacteria in this form of organization. 15,26,30 NaOCl demonstrated effectiveness in eliminating E. faecalis biofilm after short contact periods, while CHX was unable to eliminate microorganisms after 1 minute.…”

Section: 2324mentioning

confidence: 98%

“…Some dentin components may inactivate the action of endodontic antimicrobial agents. 30,[33][34][35] Therefore, CHX, CTR and QMiX may have displayed lower antibacterial action due to the presence of bovine dentin used to substrate. Biofilm elimination has been observed in other studies, where polystyrene 11 and hydroxyapatite 15 were used as substrates.…”

Section: Stojicic Et Al 15 Observed Greater Antibiofilm Activity Formentioning

confidence: 99%

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Antimicrobial Activity of Root Canal Irrigants associated with Cetrimide against Biofilm and Planktonic Enterococcus faecalis

Nascimento

Tanomaru‐Filho

Faria-Júnior

et al. 2014

The Journal of Contemporary Dental Practice

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Aim: To evaluate the antibacterial activity of sodium hypochlorite (NaOCl) and chlorhexidine (CHX) alone or associated with cetrimide (CTR), and QMiX against biofilm and planktonic Enterococcus faecalis (E. faecalis) [American type culture collection (ATCC) 29212]. Materials and methods:The solutions 2.5% NaOCl, 2.5% NaOCl + 0.2% CTR, 2% CHX, 2% CHX + 0.2% CTR, 0.2% CTR, and QMiX were evaluated. E. faecalis biofilms were induced for 14 days on bovine dentin blocks. The irrigants were evaluated after contact with E. faecalis suspension and biofilm for 1 and 3 minutes. After that, serial decimal dilutions were made and plated on tryptic soy agar (TSA) medium. Plates were incubated for 24 hours at 37°C and the colony-forming unit (CFU) 1 ml was determined. Data were subjected to ANOVA and Tukey's tests at 5% significance.Results: All microorganisms were eliminated by direct contact of the irrigants with planktonic cells. Only NaOCl and NaOCl + CTR were able to completely eliminate the microorganisms by direct contact with E. faecalis biofilm. CHX presented effectiveness similar to CHX + CTR CTR, and QMiX after 1 minute of contact and similar to NaOCl and NaOCl + CTR after 3 minutes (p > 0.05), but was unable to completely eliminate the microorganisms. CTR and QMiX did not differ from each other.Conclusion: CTR addition to CHX and NaOCl solutions did not improve the antimicrobial activity against biofilm. All evaluated irrigants and associations presented activity against planktonic E. faecalis. Only NaOCl and NaOCl + CTR eliminated biofilm after 1 and 3 minutes of direct contact.Clinical relevance: Addition of CTR does not modify the antibiofilm effectiveness of CHX and NaOCl.

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Section: 2324supporting

confidence: 73%

Section: 2324mentioning

confidence: 98%

Section: Stojicic Et Al 15 Observed Greater Antibiofilm Activity Formentioning

confidence: 99%

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Antimicrobial Activity of Root Canal Irrigants associated with Cetrimide against Biofilm and Planktonic Enterococcus faecalis

Nascimento

Tanomaru‐Filho

Faria-Júnior

et al. 2014

The Journal of Contemporary Dental Practice

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“…Cetrimide (CTR) is a cationic surfactant that reduces the surface tension of solutions, thereby improving their contact with surfaces of difficult access 12) . It was reported that incorporation of CTR into CHX solution improves the antibacterial effects of CHX 13,14) . Octenisept ® is a new antiseptic used in the treatment of traumatic, acute, chronic, surgical or burn wounds, and for mucose membrane disinfection and mouth rinses consisting of octenidine hydrochloride (OCT) and phenoxyethanol.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial effect of chlorhexidine-cetrimide combination, <i>Salvia officinalis</i> plant extract and octenidine in comparison with conventional endodontic irrigants

Güneşer

Akbulut

Eldeniz

2016

Dental Materials Journal

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“…Torabinejad et al have shown that MTAD is able to safely remove the smear layer and is effective against Enterococcus faecalis, a microorganism resistant to the action of other antimicrobial medications such as calcium hydroxide [13][14][15][16][17][18][19] . Portenier et al demonstrated that the full concentration (100%) MTAD and 0.2% chlorhexidine were both effectively killed Enterococcus faecalis 20) . The smear layer removing ability of MTAD was also investigated by recent studies.…”

Section: Introductionmentioning

confidence: 99%

The effect of MTAD as a final root canal irrigants on the coronal bacterial leakage of obturated root canals

Kim

Chang

Park

2008

J Korean Acad Conserv Dent

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The effect of MTAD as a final root canal irrigants on the coronal bacterial leakage of obturated root canalsThe purpose of this study was to evaluate the effects of MTAD, EDTA and sodium hypochlorite(NaOCl) as final irrigants on coronal leakage resistance to Enterococcus faecalis. Forty extracted human maxillary molars were used in this experiment. The teeth were randomly divided into positive control group (Group 1; n = 5), negative control group (Group 2; n = 5) and three experimental groups (n = 30). In Group 3 (n = 10), the root canals were irrigated with sodium hypochlorite. In Group 4 (n = 10) and 5 (n = 10), the root canals were irrigated with sodium hypochlorite and rinsed with EDTA and MTAD, respectively. The teeth in each group were cleaned and shaped to #40 profile with .04 taper, and obturated with gutta-percha and AH-26 root canal sealer. The coronal portion of each tooth was placed in contact with inoculum of Enterococcus faecalis in Brain Heart Infusion (BHI) culture media. Each root tip was placed in a vial containing sterile culture media. The vials were placed in anaerobic chamber and observed everyday for turbidity for 180 days. Statistical analysis was performed using Fisher's Exact Test. After 180 days, Group 3, 4, and 5 showed 7, 4 and 5 leaking samples respectively. The differences in leakage resistance were not statistically significant among Group 3, 4 and 5. [J Kor Acad Cons Dent 33(4):397-404, 2008]

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Killing of Enterococcus faecalis by MTAD and Chlorhexidine Digluconate with or without Cetrimide in the Presence or Absence of Dentine Powder or BSA

Cited by 98 publications

References 18 publications

Antimicrobial Activity of Root Canal Irrigants associated with Cetrimide against Biofilm and Planktonic Enterococcus faecalis

Antimicrobial Activity of Root Canal Irrigants associated with Cetrimide against Biofilm and Planktonic Enterococcus faecalis

Antibacterial effect of chlorhexidine-cetrimide combination, <i>Salvia officinalis</i> plant extract and octenidine in comparison with conventional endodontic irrigants

The effect of MTAD as a final root canal irrigants on the coronal bacterial leakage of obturated root canals

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