Replica of human dentin treated with different desensitizing agents: a methodological SEM study in vitro

Abstract

This is a preliminary study to determine a methodological sequence in vitro which may allow the reproduction of dentin for SEM analysis, after the use of different desensitizing agents. Dentin discs obtained from extracted human third molars were etched with 6% citric acid, an artificial smear layer was created and the surface dentin discs were divided into four quadrants. Quadrants 2, 3 and 4 of each disc were conditioned with 6% citric acid. The desensitizing agents (Oxa-Gel ® , Gluma Desensitizer and an experimental agent) were applied to quadrants 3 and 4. To evaluate the acid resistance of the treatment, quadrant 4 was etched again with 6% citric acid. An impression was then taken with Aquasil ULV. After a setting period of 6 min, each disc was removed from the impression and stored in a moist-free environment for 24 h at 37 o C. After that time, a low-viscosity epoxy resin (Araltec GY 1109 BR) was poured into the impression and cured for 24 h. All specimens were metal-coated for SEM analysis. Comparison of the photomicrographs of dentin discs with their respective impressions and resin replicas showed that this technique can reproduce the characteristics of the dentin surface treated with desensitizing agents.

Evaluation of mineral content of dentin treated with desensitizing agents and neodymium yttrium-aluminium-garnet (Nd:YAG) laser

“…As it does not include chemicals to produce polymerization, the desensitizing effects of Admira Protect are thought to occur by precipitation of plasma proteins of dentinal fluid inside the tubules, thereby reducing fluid flow. Clinically, Admira Protect acts as a primer, creating multiple intratubular septa layers in the lumen of the dentinal tubules as a result of The dentin is partially obliterated due to solidification of melted dentin protein precipitation [32]. Systemp contains polyethylene glycol dimethacrylate and glutaraldehyde in an aqueous solution.…”

Dentin hypersensitivity after treatment with desensitizing agents: a randomized, double-blind, split-mouth clinical trial

“…This mechanism explains why desensitizing agents usually act in short-and longterm bases (11,12). Calcium chloride and potassium phosphate solutions have been tested in vitro (13,14) and in vivo (15), and may work similarly. Both approaches have shown very promising results in treating dentin hypersensitivity.…”

Comparative In Vitro Effect of TiF4 to NaF and Potassium Oxalate on Reduction of Dentin Hydraulic Conductance

“…2 According to this theory, any decrease in dentinal fluid movement (conductance) should result in reduced DH. 3 Many products have been developed to alleviate this clinical condition, such as potassium oxalate, fluoride, laser, and other agents with dentin occlusive effects. 4,5 The action of potassium oxalate in diminishing DH is well known.…”