Development of an amperometric enzyme electrode based on poly(o-phenylenediamine) for the determination of total cholesterol in serum

Abstract

Um eletrodo enzimático amperométrico baseado em polímero foi preparado para detecção amperométrica de colesterol livre. Inicialmente, o filme polimérico poli(o-fenilenodiamina) (PoPD) foi eletropolimerizado no meio acetonitrila-água contendo o monômero o-fenilenodiamina (oPD) e ácido canforsulfônico em eletrodo de Pt através da técnica de voltametria cíclica. A enzima oxidase colesterol foi imobilizada sobre a superfície do eletrodo Pt/PoPD. A determinação de colesterol foi realizada por maio do monitoramento da corrente de oxidação de H 2 O 2 produzido enzimaticamente a +0,7 V vs. Ag/AgCl. A concentração da solução tampão, o valor de pH e a temperatura de trabalho otimizados foram 0,05 mol L −1 , 7,5 and 40 °C, respectivamente. O intervalo de trabalho do eletrodo de enzima para colesterol foi 9,8 × 10 −3 -11 µmol L −1 e tempo de resposta 150 s. Os efeitos de possíveis interferências presentes em amostras de soro em resposta ao eletrodo de enzima foram examinados. A determinação do colesterol total em amostras de soro foi realizada usando o eletrodo de enzima Pt/PoPD/ChOx proposto e os resultados foram em bom acordo com os obtidos pelo método espectrofotométrico.A polymer-based amperometric enzyme electrode (Pt/PoPD/ChOx) was prepared for the amperometric detection of free cholesterol. Firstly, poly(o-phenylenediamine) (PoPD) polymer film was prepared in acetonitrile-water medium containing o-phenylenediamine (oPD) monomer and (±)-10-camphorsulfonic acid (HCSA) on Pt electrode by the use of cyclic voltammetry technique. Cholesterol oxidase (ChOx) enzyme was immobilized onto Pt/PoPD electrode surface. The determination of cholesterol was performed via monitoring of the oxidation current of enzymatically produced H 2 O 2 at +0.7 V vs. Ag/AgCl. Optimum buffer concentration, pH and working temperature were found as 0.05 mol L −1 , 7.5 and 40 °C, respectively. The working range of enzyme electrode to cholesterol was 9.8 × 10 −3 -11 µmol L −1 and response time 150 s. The effects of possible interferences present in serum samples on response of enzyme electrode were examined. The determination of total cholesterol in serum samples was performed by using proposed Pt/PoPD/ChOx enzyme electrode and results were in good agreement with those obtained by spectrophotometric method.

Electrochemical aptasensor for mucin 1 based on dual signal amplification of poly(o-phenylenediamine) carrier and functionalized carbon nanotubes tracing tag

“…In recent years, poly(o-phenylenediamine) (PoPD), as one of the most interesting polyanilines, has been proven to be used as a functional polymer in many studies due to its positively charged surface, homogeneity in electrochemical deposition and strong adherence to electrode surface (Turkmen et al, 2014;Wen et al, 2014;Duan et al, 2013;Khan and Ab Ghani, 2012). In addition, PoPD film could yield an interface with amino groups for the subsequent assembly of noble metal nanoparticles to enhance the conductivity (Turkmen et al, 2014;Kong et al, 2013;Zeybek et al, 2012;Zeybek et al, 2014). Among the noble metallic nanomaterials, gold nanoparticles (AuNPs) have become the most widely used due to its advantages of easy synthesis, low cost, good biocompatibility and ability to be readily modified by various chemical methods (Daniel and Astruc, 2004;Ghosh et al, 2008).…”

Metal-based non-enzymatic systems for cholesterol detection: mechanisms, features, and performance

“…In the redox mediator based enzymatic detection techniques, ferrocene, 7 methylene blue, 8 Prussian blue, 52 hydroquinone, 53 poly( o -phenylenediamine), 54 and (hydroxymethyl)ferrocene 55 were reported to be implemented in the system. Here, the regeneration of the reduced form of the redox mediators by electrical potential is utilized for the detection process.…”

Green synthesis of poly(o‐phenylenediamine) polymer film on stainless steel as a corrosion protection layer

“…In the field of anticorrosion, conductive polymers have broad application prospects in ferrous and nonferrous materials 4–9 . In other aspects have also been widely concerned like sensor and battery 10–12 . The conductive polymers are used as coatings or inhibitors to protect metal substrates in corrosive media.…”

“…[4][5][6][7][8][9] In other aspects have also been widely concerned like sensor and battery. [10][11][12] The conductive polymers are used as coatings or inhibitors to protect metal substrates in corrosive media. Researchers also believe that the structural properties of polymers have a certain relationship with corrosion resistance.…”