Activity of the Lupane isolated from Combretum leprosum against Leishmania amazonensis promastigotes

Teles, Carolina Bioni Garcia; Moreira, Leandro Soares; Facundo, Valdir Alves; Zuliani, Juliana Pavan; Stábeli, Rodrigo G.; Silva, Alexandre de Almeida e

doi:10.1590/s0103-50532011000500017

Cited by 29 publications

(26 citation statements)

References 30 publications

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“…The ethanolic extract was tested on a 25 μg/mL concentration and the lupane was tested on a 5 μg/ mL for citotoxicity. On these concentrations, neither the extract nor the lupane were toxic (TELES et al, 2011). The citotoxicity result of the ethanolic extract for Leishmania is also in agreement with our work.…”

Section: Resultssupporting

confidence: 92%

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In Vitro Antiplasmodial Activity of Flower Extracts From Combretum Leprosum Mart (Mofumbo)

Passarini

Medeiros

Meneguetti

et al. 2016

CeN

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Malaria is the cause of hundreds of thousands of deaths per year, besides putting billions of people at risk of developing disease. When it comes to its therapy, the drugs used currently are losing its efficacy due to increase in the frequency of resistant strains of the parasite, highlighting the importance for the search of new classes of molecules presenting antiplasmodial activity. In the present work, the antiplasmodial activities of five extracts (crude, hexane, chloroform, ethyl acetate and methanol) from the flowers of Combretum leprosum are assessed. The method employed for obtaining the extracts was silica gel column chromatography, and the techniques used for the analysis of antiplasmodial activity (IC 50 ) and citotoxicity (MLD 50 ) were ELISA and MTT respectively, where a selectivity index was calculated after the obtaining of these two values. The extract presenting the highest antiplasmodial activity was the chloroform extract (IC 50 = 3.97 µg/mL), however, this extract also presented the higher cytotoxicity (MLD 50 = 18.12 µg/mL), and therefore the extract presenting the best overall activity was the methanolic extract (IS = 18.97 IntrodutionOver 3 billion people are estimated to be at risk of being infected with malaria and therefore developing disease, and according to latest estimates, hundreds of thousands of deaths caused by it occurred in each year of this millennium (WHO, 2014). The world region that suffers the heaviest burden is Africa, where the great majority of cases of malaria occur, mainly in children under the age of five (WHO, 2014). The disease is caused by parasitic protozoa of the genus Plasmodium, of which five species are known to infect humans: P. falciparum, P. vivax, P. malariae, P. ovale, and P. knowlesi (COX, 2010). The parasite life cycle begins when a female mosquito of Anopheles genus bites a human and releases the sporozoites from its salivary glands to the dermis, where they reach the avascular tissue and then a percentage of them migrates and enters the blood vessels, making their way to the liver, where they undergo cellular modifications and perform rounds of asexual replication (ALY; VAUGHAN; KAPPE, 2009).After leaving the liver, the parasite begins other cycles of asexual replication inside red blood cells, using molecules and cellular structures of the host cells to survive, grow and reproduce, generating thousands of merozoites and causing the characteristic symptoms of the disease (TILLEY; DIXON; KIRK, 2011). When it comes to its chemotherapy, most of the cases of uncomplicated malaria are treated currently by the use of ACT's drugs, although is already known that these drugs are losing its efficacy due to the increase in frequency of resistant strains (VISSER; VUGT; GROBUSCH, 2014), which highlights the importance for the search of new molecules bearing antiplasmodial activity.Plants have been used during millennia by humanity, and pharmacological studies of medicinal plants yielded useful molecules in the treatment of various diseases, includ...

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Section: Resultssupporting

confidence: 92%

“…Triterpenes that were isolated from the genus Combretum also showed activities against another microorganisms, like fungi (BISOLI et al, 2008), bacteria (ANGEH et al, 2007a;ANGEH et al, 2007b;SONGCA, RAMURAFHI;OLUWAFEMI, 2013), viruses (ASRES;BUCAR, 2005) and other species of parasites (TELES et al, 2011;HAAVIKO et al, 2014).…”

Section: Resultsmentioning

confidence: 99%

In Vitro Antiplasmodial Activity of Flower Extracts From Combretum Leprosum Mart (Mofumbo)

Passarini

Medeiros

Meneguetti

et al. 2016

CeN

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“…The inhibiting activity of triterpenoids isolated is described for promastigotes [28,29] and intracellular amastigotes of Leishmania amazonensis [28]. amazonensis was inhibited (IC 50 = 67 µM) [31]. The anti-leishmanial activity of hexane fractions from M. linifera may be related to sequiterpenes and other terpenes ( Table 2 and Table 3).…”

Section: Discussionmentioning

confidence: 96%

Anti-promastigote Activity of the Amazon Plants

Silva-Silva¹,

Monteiro²,

Brígido³

et al. 2017

JPP

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This study evaluated in vitro activity of ethanol extract, fractions, and isolated substance from Amazon species against promastigotes of L. amazonensis. The ethanol extracts were concentrated and fractionation. The anti-promastigote activity was evaluated through the cell viability assessment method (MTT). The ethanol extract, fractions, and isolated substance from Himatanthus articulatus and Parahancornia fasciculata were inactive in promastigote of L. amazonensis, as the ethanol extract of Physalis angulata. The hexane fractions from different parts of Montrichardia linifera showed anti-promastigote activity probably due to the presence of steroids and terpenes. All species in studies were inactive, except of M. linifera. The few polar constituents can be responsible for the activity. Therefore, the isolation and purification of the active on L. amazonensis promastigotes are urgently required.

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“…Torres-Santos et al (26) and Teles et al (29) investigated the effects of isolated triterpenes on promastigotes of L. amazonensis and found that the presence of the carboxylic acid grouping was associated with antiprotozoal activity. Pentacyclic triterpenes, with more hydroxyl groups, had greater effects on these promastigotes and this chemical characteristic is also present in tingenone B, which is a pentacyclic triterpene with a quinonamethide skeleton.…”

Section: Discussionmentioning

confidence: 99%

Screening of the in vitro antileishmanial activities of compounds and secondary metabolites isolated from Maytenus guianensis Klotzsch ex Reissek (Celastraceae) chichuá Amazon

Meneguetti

Lima

Hurtado

et al. 2016

Rev. Soc. Bras. Med. Trop.

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Introduction:Maytenus guianensis is a member of the Celastraceae family that is used in traditional medicine, particularly for its anti-parasitic and anti-cancer effects. To explore the ethnopharmacological potential of this plant, the present study was designed to screen the in vitro antileishmanial activities of extracts and compounds isolated from M. guianensis. Methods: Maytenus guianensis stems and leaves were extracted in acetone, followed by the preparation of eluates and isolation of secondary metabolites using chromatography on a glass column with silica gel as the fixed phase. The chemical components were identified using spectroscopic methods, including one-and two-dimensional nuclear magnetic resonance of hydrogen-1 and carbon-13, mass spectroscopy, and infrared spectroscopy. The anti-Leishmania amazonensis activities of these eluates and compounds were evaluated by direct promastigote counting and viability assays. Results: It was found that the hexane bark eluate produced the strongest anti-L. amazonensis effect, with 90-100% inhibition of the promastigote form. The isolated metabolite that produced the best result was tingenone B, followed by a compound formed by the union of tingenone and tingenone B (80-90% inhibition). Conclusions: Maytenus guianensis shows anti-parasite activity that warrants further investigation to determine the mechanisms underlying this antileishmanial effect and to evaluate the pharmacological potential of these eluates and isolated secondary metabolites, while minimizing any adverse effects.

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Activity of the Lupane isolated from Combretum leprosum against Leishmania amazonensis promastigotes

Cited by 29 publications

References 30 publications

In Vitro Antiplasmodial Activity of Flower Extracts From Combretum Leprosum Mart (Mofumbo)

In Vitro Antiplasmodial Activity of Flower Extracts From Combretum Leprosum Mart (Mofumbo)

Anti-promastigote Activity of the Amazon Plants

Screening of the in vitro antileishmanial activities of compounds and secondary metabolites isolated from Maytenus guianensis Klotzsch ex Reissek (Celastraceae) chichuá Amazon

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