Composition and antimicrobial activity of essential oils from Poiretia bahiana C. Müller (Papilionoideae-Leguminosae)

Abstract

A espécie Poiretia bahiana, popularmente conhecida como arrudinha, arruda da serra ou arruda brava, tem uma ocorrência restrita aos campos rupestres da Chapada Diamantina, BA. Suas partes aéreas apresentam um aroma pungente e é usada pelo povo do local no controle de infestações por pulgas e no tratamento de hemorróidas, dores nas articulações e sinusite. Neste trabalho, relatamos a composição dos óleos voláteis obtidos das partes aéreas de cinco espécimes e do óleo volátil obtido dos frutos de um espécime de P. bahiana, os quais foram coletados em diferentes regiões da Chapada Diamantina. Nos óleos voláteis que foram analisados por CG-FID e CG-EM foram identificados vinte e nove componentes sendo que os dois principais foram a umbelulona, em concentrações entre 55,0% e 75,3%, e sabineno, em concentrações entre 10,3% e 25,2%. O óleo das partes aéreas de um espécime e o óleo dos frutos de outro espécime foram avaliados quanto às suas propriedades antimicrobianas contra seis bactérias, duas leveduras e cinco fungos filamentosos pelos ensaios de difusão em ágar e microdiluição. Os óleos exibiram atividade contra Escherichia coli, Salmonella choleraesuis, Proteus mirabilis, Staphylococcus aureus, Staphylococcus aureus resistente à meticilina (MRSA), Candida albicans, Cryptococcus neoformans, Cladosporium herbarum, Microsporum canis, Trichophyton rubrum e foram inativos contra Pseudomonas aeruginosa, Aspergillus fumigatus e Penicillium notatum. A toxicidade do óleo das partes aéreas foi determinada contra Artemia salina apresentando uma LC 50 de 459,03 mg mL -1 .Poiretia bahiana known as "arrudinha", "arruda da serra" or "arruda brava", has its occurrence restricted to the rock fields (campo rupestre) areas in Chapada Diamantina, Bahia, northeastern region of Brazil. Its aerial parts present a pungent smell and have been used by local communities as fumigant for the control of infestation of fleas and in the treatment of hemorrhoids, articulations pain and sinusitis. This work reports the composition of the volatile oils from aerial parts of five specimens and of the essential oil from the fruits of one specimen of Poiretia bahiana that were harvested in four different places of Chapada Diamantina. Twenty nine components were identified from the oils analyzed by GC-FID and GC-MS. Umbellulone, in concentrations between 55.0% and 75.3% and sabinene, in concentrations between 10.3% and 25.2% are the major components. The oil from the aerial parts of one specimen and the oil of fruits of another specimen had their antimicrobial properties evaluated against six bacteria, two yeasts and five filamentous fungi by the agar diffusion method and microdilution assay. The in vitro results demonstrated that these essential oils exhibit antimicrobial activity against Escherichia coli, Salmonella choleraesuis, Proteus mirabilis, Staphylococcus aureus, methicilin resistant Staphylococcus aureus (MRSA), Candida albicans, Cryptococcus neoformans, Cladosporium herbarum, Microsporum canis, Trichophyton rubrum and are inactive against Pse...

In Vitro and In Vivo Anti-Candida spp. Activity of Plant-Derived Products

“…Exposure to the Cupressus sempervirens EO completely inhibited C. orthopsilosis (Figure 1F) and C. glabrata (Figure 1G) cells. The fungicidal action of this EO could be partly attributed to its major constituents such as sabinene, citral, and terpinen-4-ol, which have been reported to display antimicrobial effects [22,23,24]. Moreover, at 2 x MIC, this EO provided the same effect as 4 μg/mL AMB against C. orthopsilosis (Figure 1F), which is the best antifungal drug concentration with fungicidal action that has been described in in vivo studies [25].…”

Antibacterial activity of native California medicinal plant extracts isolated from Rhamnus californica and Umbellularia californica

“…Specifically, a structural analog of the anthraquinone emodin, found in the genus Rhamnus , and a novel class of synthetic antibacterial cationic anthraquinones have shown potent antibacterial effects against MRSA [ 26 , 27 ]. Likewise, structural analogs of flavonoids kaempferol, quercetin and eriodictyol [ 28 ], found in U. californica , and plant extracts containing terpenoids umbellulone, 1,8-cineole, α-terpineol and thymol among others [ 29 – 31 ] have shown anti-MRSA activity. Interestingly, even though 1,8-cineole exhibits little inherent antimicrobial activity, it has been shown to enhance the potency of other antimicrobials against MRSA [ 31 ].…”

Properties of constituents from Maytenus gonoclada against Entamoeba histolytica and two leukemia cell strains

“…This property facilitated the diffusion of compound 1 in the culture medium, generating good conditions to reach the highest activity. The polarity of the substances is important for its antimicrobial activity, due to the diffusion in biological medium (Araújo et al, 2009). The low polarity of triterpenes 2 and 3 suggested that the lack of activity against E. histolytica may be related to its insolubility in the culture medium.…”